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• W2112012022 abstract "Abstract The application of microfluidic devices in diagnostic systems is well-established in contemporary research. Large specific surface area of microspheres, on the other hand, has secured an important position for their use in bioanalytical assays. Herein, we report a combination of microspheres and microfluidic disk in a unique hybrid platform for highly sensitive and selective detection of dengue virus. Surface engineered polymethacrylate microspheres with carefully designed functional groups facilitate biorecognition in a multitude manner. In order to maximize the utility of the microspheres’ specific surface area in biomolecular interaction, the microfluidic disk was equipped with a micromixing system. The mixing mechanism (microballoon mixing) enhances the number of molecular encounters between spheres and target analyte by accessing the entire sample volume more effectively, which subsequently results in signal amplification. Significant reduction of incubation time along with considerable lower detection limits were the prime motivations for the integration of microspheres inside the microfluidic disk. Lengthy incubations of routine analytical assays were reduced from 2 hours to 5 minutes while developed system successfully detected a few units of dengue virus. Obtained results make this hybrid microsphere-microfluidic approach to dengue detection a promising avenue for early detection of this fatal illness." @default.
• W2112012022 created "2016-06-24" @default.
• W2112012022 creator A5009610104 @default.
• W2112012022 creator A5010681619 @default.
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• W2112012022 creator A5071652002 @default.
• W2112012022 creator A5073716145 @default.
• W2112012022 creator A5073733053 @default.
• W2112012022 date "2015-11-09" @default.
• W2112012022 modified "2023-10-16" @default.
• W2112012022 title "Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection" @default.
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• W2112012022 doi "https://doi.org/10.1038/srep16485" @default.
• W2112012022 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4637926" @default.
• W2112012022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26548806" @default.
• W2112012022 hasPublicationYear "2015" @default.
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