Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112020232> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2112020232 endingPage "531a" @default.
- W2112020232 startingPage "531a" @default.
- W2112020232 abstract "Introduction: A decrease in mitochondrial pH (pHm) inhibits mitochondrial permeability transition pore (mPTP) and has been associated with cardioprotection. The volatile anesthetic isoflurane decreases mitochondrial membrane potential (ΔΨm) and inhibits respiratory complex I. However, the effect of isoflurane on pHm is unknown. We hypothesized that exposure to isoflurane lowers pHm in cardiomyocytes and isolated mitochondria. Methods: The direct effect of 0.5 mM isoflurane (1 MAC) was tested in cardiomyocytes and mitochondria isolated from adult male Wistar rats. We used fluorescence dyes SNARF-1 and BCECF to measure pHm in myocytes with a confocal microscope and in mitochondria with a spectrofluorometer, respectively. In myocytes, after baseline recording, cells were superfused with isoflurane for 5 min. In mitochondria, isoflurane was added to mitochondria in the presence of pyruvate/malate (5 mM) and ADP (250 μM ADP). Respiration was recorded using a Clark-type electrode with pyruvate/malat as substrate. Results: Exposure of myocytes to isoflurane decreased pHm 0.09± 0.03 pH units (P<0.05, n=5). In mitochondria, isoflurane induced decrease in pHm was 8 ± 2% (P<0.05, n=5) of maximal acidification induced by mitochondrial uncoupler FCCP (4 μM). This effect was mimicked when mitochondria were exposed to rotenone (5 μM). Isoflurane inhibited pyruvat/malat-dependent oxygen consumption in the presence and absence of ADP. Conclusion: These results suggest that isoflurane decreases pHm through inhibition of complex I of the electron transport chain. Isoflurane-induced acidification may contribute to the immediate protective effect provided by volatile anesthetics when applied at the onset of cardiac reperfusion after an ischemic event." @default.
- W2112020232 created "2016-06-24" @default.
- W2112020232 creator A5024234611 @default.
- W2112020232 creator A5035697355 @default.
- W2112020232 creator A5067283559 @default.
- W2112020232 creator A5070298094 @default.
- W2112020232 date "2009-02-01" @default.
- W2112020232 modified "2023-10-05" @default.
- W2112020232 title "Direct Effect of Isoflurane on Mitochondrial pH" @default.
- W2112020232 doi "https://doi.org/10.1016/j.bpj.2008.12.2739" @default.
- W2112020232 hasPublicationYear "2009" @default.
- W2112020232 type Work @default.
- W2112020232 sameAs 2112020232 @default.
- W2112020232 citedByCount "0" @default.
- W2112020232 crossrefType "journal-article" @default.
- W2112020232 hasAuthorship W2112020232A5024234611 @default.
- W2112020232 hasAuthorship W2112020232A5035697355 @default.
- W2112020232 hasAuthorship W2112020232A5067283559 @default.
- W2112020232 hasAuthorship W2112020232A5070298094 @default.
- W2112020232 hasBestOaLocation W21120202321 @default.
- W2112020232 hasConcept C12554922 @default.
- W2112020232 hasConcept C126322002 @default.
- W2112020232 hasConcept C134018914 @default.
- W2112020232 hasConcept C13591479 @default.
- W2112020232 hasConcept C178790620 @default.
- W2112020232 hasConcept C181911157 @default.
- W2112020232 hasConcept C185592680 @default.
- W2112020232 hasConcept C190283241 @default.
- W2112020232 hasConcept C207200792 @default.
- W2112020232 hasConcept C2776359302 @default.
- W2112020232 hasConcept C2778162923 @default.
- W2112020232 hasConcept C2778389096 @default.
- W2112020232 hasConcept C2779676291 @default.
- W2112020232 hasConcept C28859421 @default.
- W2112020232 hasConcept C31573885 @default.
- W2112020232 hasConcept C42219234 @default.
- W2112020232 hasConcept C541997718 @default.
- W2112020232 hasConcept C55493867 @default.
- W2112020232 hasConcept C71924100 @default.
- W2112020232 hasConcept C86803240 @default.
- W2112020232 hasConcept C98274493 @default.
- W2112020232 hasConceptScore W2112020232C12554922 @default.
- W2112020232 hasConceptScore W2112020232C126322002 @default.
- W2112020232 hasConceptScore W2112020232C134018914 @default.
- W2112020232 hasConceptScore W2112020232C13591479 @default.
- W2112020232 hasConceptScore W2112020232C178790620 @default.
- W2112020232 hasConceptScore W2112020232C181911157 @default.
- W2112020232 hasConceptScore W2112020232C185592680 @default.
- W2112020232 hasConceptScore W2112020232C190283241 @default.
- W2112020232 hasConceptScore W2112020232C207200792 @default.
- W2112020232 hasConceptScore W2112020232C2776359302 @default.
- W2112020232 hasConceptScore W2112020232C2778162923 @default.
- W2112020232 hasConceptScore W2112020232C2778389096 @default.
- W2112020232 hasConceptScore W2112020232C2779676291 @default.
- W2112020232 hasConceptScore W2112020232C28859421 @default.
- W2112020232 hasConceptScore W2112020232C31573885 @default.
- W2112020232 hasConceptScore W2112020232C42219234 @default.
- W2112020232 hasConceptScore W2112020232C541997718 @default.
- W2112020232 hasConceptScore W2112020232C55493867 @default.
- W2112020232 hasConceptScore W2112020232C71924100 @default.
- W2112020232 hasConceptScore W2112020232C86803240 @default.
- W2112020232 hasConceptScore W2112020232C98274493 @default.
- W2112020232 hasIssue "3" @default.
- W2112020232 hasLocation W21120202321 @default.
- W2112020232 hasOpenAccess W2112020232 @default.
- W2112020232 hasPrimaryLocation W21120202321 @default.
- W2112020232 hasRelatedWork W1966102603 @default.
- W2112020232 hasRelatedWork W2014941665 @default.
- W2112020232 hasRelatedWork W2022789045 @default.
- W2112020232 hasRelatedWork W2027355106 @default.
- W2112020232 hasRelatedWork W2029294373 @default.
- W2112020232 hasRelatedWork W2071925317 @default.
- W2112020232 hasRelatedWork W2112020232 @default.
- W2112020232 hasRelatedWork W2126475226 @default.
- W2112020232 hasRelatedWork W2181957617 @default.
- W2112020232 hasRelatedWork W2921580252 @default.
- W2112020232 hasVolume "96" @default.
- W2112020232 isParatext "false" @default.
- W2112020232 isRetracted "false" @default.
- W2112020232 magId "2112020232" @default.
- W2112020232 workType "article" @default.