FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112089723> ?p ?o ?g. }

• W2112089723 endingPage "464" @default.
• W2112089723 startingPage "457" @default.
• W2112089723 abstract "The ability of lysozyme to aggregate and lyse the gram-negative capnophilic periodontal microorganism Capnocytophaga gingivalis 2010 was monitored optically at 540 nm. Both hen egg white and chromatographically purified human lysozymes had significant but similar aggregation potentials for both logarithmic- and stationary-phase bacteria. In general, an increase in enzyme concentration resulted in a graded increase in both the initial and maximum changes in turbidity which occurred during the reaction period. The greatest change in turbidity occurred within the initial minutes of interaction of lysozyme and the cells, and the extent of aggregation paralleled a rapid depletion of lysozyme by the suspensions during the first minute of its incubation with the bacteria. Interestingly, the muramidase inhibitors N-acetyl-D-glucosamine and histamine did not block aggregation, whereas maleylation of lysozyme completely inhibited its aggregating ability. Demaleylation, however, restored aggregation activity comparable to the native enzyme, indicating that maleylated lysozyme retained its integrity and that aggregation was primarily dependent on charge. The addition of up to physiological concentrations of NaHCO3 and NaCl to cell aggregates resulted in varying degrees of deaggregation and lysis. Surprisingly, ultrastructural analysis of lysozyme-treated cells revealed morphological changes with or without the addition of salt. Damage appeared to occur at the blunted polar end of the cells where there was a large spherical outpouching bordered by a damaged cell envelope. Damaged cells uniformly contained dense granular cytoplasmic debris. In effect, the cationic enzyme lysed C. gingivalis 2010, which was not apparent in the spectrophotometric assay. The paradoxical finding that during bacterial aggregation there was lysis may be of significance to the further elucidation of lysozyme's antibacterial role in the gingival sulcus." @default.
• W2112089723 created "2016-06-24" @default.
• W2112089723 creator A5013091632 @default.
• W2112089723 creator A5014555023 @default.
• W2112089723 creator A5034936181 @default.
• W2112089723 creator A5042440777 @default.
• W2112089723 creator A5063969692 @default.
• W2112089723 creator A5072072813 @default.
• W2112089723 date "1985-02-01" @default.
• W2112089723 modified "2023-10-14" @default.
• W2112089723 title "Lysozyme-mediated aggregation and lysis of the periodontal microorganism Capnocytophaga gingivalis 2010" @default.
• W2112089723 cites W1496461131 @default.
• W2112089723 cites W1506041395 @default.
• W2112089723 cites W1559583668 @default.
• W2112089723 cites W1580496446 @default.
• W2112089723 cites W1593609072 @default.
• W2112089723 cites W1661860066 @default.
• W2112089723 cites W1774235504 @default.
• W2112089723 cites W1845949782 @default.
• W2112089723 cites W1889381613 @default.
• W2112089723 cites W1893417737 @default.
• W2112089723 cites W1929053701 @default.
• W2112089723 cites W1936043448 @default.
• W2112089723 cites W1946761825 @default.
• W2112089723 cites W1952753155 @default.
• W2112089723 cites W1957749435 @default.
• W2112089723 cites W1977802642 @default.
• W2112089723 cites W1984033049 @default.
• W2112089723 cites W1986728490 @default.
• W2112089723 cites W1996533910 @default.
• W2112089723 cites W1999537188 @default.
• W2112089723 cites W2004842045 @default.
• W2112089723 cites W2005477377 @default.
• W2112089723 cites W200577346 @default.
• W2112089723 cites W2015245271 @default.
• W2112089723 cites W2019084198 @default.
• W2112089723 cites W2026589019 @default.
• W2112089723 cites W2026721162 @default.
• W2112089723 cites W2028945742 @default.
• W2112089723 cites W2035756994 @default.
• W2112089723 cites W2038513609 @default.
• W2112089723 cites W2057121756 @default.
• W2112089723 cites W2062101403 @default.
• W2112089723 cites W2063427484 @default.
• W2112089723 cites W2066408540 @default.
• W2112089723 cites W2066806541 @default.
• W2112089723 cites W2077191163 @default.
• W2112089723 cites W2079790969 @default.
• W2112089723 cites W2086204490 @default.
• W2112089723 cites W2096931949 @default.
• W2112089723 cites W2118035901 @default.
• W2112089723 cites W2121670067 @default.
• W2112089723 cites W2130964140 @default.
• W2112089723 cites W2141479958 @default.
• W2112089723 cites W2142304645 @default.
• W2112089723 cites W2146456204 @default.
• W2112089723 cites W2147673321 @default.
• W2112089723 cites W2150683262 @default.
• W2112089723 cites W2168705616 @default.
• W2112089723 cites W2177492968 @default.
• W2112089723 cites W2318441237 @default.
• W2112089723 cites W2319495509 @default.
• W2112089723 cites W2479454318 @default.
• W2112089723 cites W2496820439 @default.
• W2112089723 cites W28819135 @default.
• W2112089723 doi "https://doi.org/10.1128/iai.47.2.457-464.1985" @default.
• W2112089723 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/263192" @default.
• W2112089723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3967924" @default.
• W2112089723 hasPublicationYear "1985" @default.
• W2112089723 type Work @default.
• W2112089723 sameAs 2112089723 @default.
• W2112089723 citedByCount "29" @default.
• W2112089723 countsByYear W21120897232015 @default.
• W2112089723 countsByYear W21120897232016 @default.
• W2112089723 countsByYear W21120897232017 @default.
• W2112089723 countsByYear W21120897232018 @default.
• W2112089723 countsByYear W21120897232019 @default.
• W2112089723 countsByYear W21120897232020 @default.
• W2112089723 countsByYear W21120897232021 @default.
• W2112089723 crossrefType "journal-article" @default.
• W2112089723 hasAuthorship W2112089723A5013091632 @default.
• W2112089723 hasAuthorship W2112089723A5014555023 @default.
• W2112089723 hasAuthorship W2112089723A5034936181 @default.
• W2112089723 hasAuthorship W2112089723A5042440777 @default.
• W2112089723 hasAuthorship W2112089723A5063969692 @default.
• W2112089723 hasAuthorship W2112089723A5072072813 @default.
• W2112089723 hasBestOaLocation W21120897231 @default.
• W2112089723 hasConcept C12554922 @default.
• W2112089723 hasConcept C181199279 @default.
• W2112089723 hasConcept C185592680 @default.
• W2112089723 hasConcept C2776966407 @default.
• W2112089723 hasConcept C523546767 @default.
• W2112089723 hasConcept C54355233 @default.
• W2112089723 hasConcept C55493867 @default.
• W2112089723 hasConcept C57409179 @default.
• W2112089723 hasConcept C86803240 @default.
• W2112089723 hasConcept C89423630 @default.
• W2112089723 hasConceptScore W2112089723C12554922 @default.

• next