FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112432133> ?p ?o ?g. }

• W2112432133 endingPage "197" @default.
• W2112432133 startingPage "185" @default.
• W2112432133 abstract "Plasmid DNA pRc/CMV HBS encoding the S (small) region of hepatitis B surface antigen (HBsAg) was incorporated by the dehydration-rehydration method into Lipodine liposomes composed of 16 micro moles phosphatidylcholine (PC) or distearoyl phosphatidylcholine (DSPC), 8 micro moles of (dioleoyl phosphatidylethanolamine (DOPE) or cholesterol and 4 micro moles of the cationic lipid 1,2-dioleoyl-3-(trimethylammonium propane (DOTAP) (molar ratios 1 : 0.5 : 0.25). Incorporation efficiency was high (89-93% of the amount of DNA used) in all four formulations tested and incorporated DNA was shown to be resistant to displacement in the presence of the competing anionic sodium dodecyl sulphate molecules. This is consistent with the notion that most of the DNA is incorporated within the multilamellar vesicles structure rather than being vesicle surface-complexed. Stability studies performed in simulated intestinal media also demonstrated that dehydration-rehydration vesicles (DRV) incorporating DNA (DRV(DNA)) were able to retain significantly more of their DNA content compared to DNA complexed with preformed small unilamellar vesicles (SUV-DNA) of the same composition. Moreover, after 4h incubation in the media, DNA loss for DSPC DRV(DNA) was only minimal, suggesting this to be the most stable formulation. Oral (intragastric) liposome-mediated DNA immunisation studies employing a variety of DRV(DNA) formulations as well as naked DNA revealed that secreted IgA responses against the encoded HBsAg were (as early as three weeks after the first dose) substantially higher after dosing with 100 micro g liposome-entrapped DNA compared to naked DNA. Throughout the fourteen week investigation, IgA responses in mice were consistently higher with the DSPC DRV(DNA) liposomes compared to naked DNA and correlated well with their improved DNA retention when exposed to model intestinal fluids. To investigate gene expression after oral (intragastric) administration, mice were given 100 micro g of naked or DSPC DRV liposome-entrapped plasmid DNA expressing the enhanced green fluorescent protein (pCMV.EGFP). Expression of the gene, in terms of fluorescence intensity in the draining mesenteric lymph nodes, was much greater in mice dosed with liposomal DNA than in animals dosed with the naked DNA. These results suggest that DSPC DRV liposomes containing DNA (Lipodine) may be a useful system for the oral delivery of DNA vaccines." @default.
• W2112432133 created "2016-06-24" @default.
• W2112432133 creator A5010654465 @default.
• W2112432133 creator A5011580238 @default.
• W2112432133 creator A5016971703 @default.
• W2112432133 creator A5059203004 @default.
• W2112432133 date "2002-01-01" @default.
• W2112432133 modified "2023-09-23" @default.
• W2112432133 title "LIPOSOME (LIPODINE™)-MEDIATED DNA VACCINATION BY THE ORAL ROUTE" @default.
• W2112432133 cites W1504398954 @default.
• W2112432133 cites W1505248622 @default.
• W2112432133 cites W1511118087 @default.
• W2112432133 cites W1563698861 @default.
• W2112432133 cites W1963895695 @default.
• W2112432133 cites W1970502718 @default.
• W2112432133 cites W1988925907 @default.
• W2112432133 cites W1990635325 @default.
• W2112432133 cites W2063784258 @default.
• W2112432133 cites W2068953057 @default.
• W2112432133 cites W2072090144 @default.
• W2112432133 cites W2098073954 @default.
• W2112432133 cites W2153156984 @default.
• W2112432133 cites W2161317598 @default.
• W2112432133 cites W4299608669 @default.
• W2112432133 doi "https://doi.org/10.1081/lpr-120004792" @default.
• W2112432133 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12604053" @default.
• W2112432133 hasPublicationYear "2002" @default.
• W2112432133 type Work @default.
• W2112432133 sameAs 2112432133 @default.
• W2112432133 citedByCount "56" @default.
• W2112432133 countsByYear W21124321332012 @default.
• W2112432133 countsByYear W21124321332013 @default.
• W2112432133 countsByYear W21124321332014 @default.
• W2112432133 countsByYear W21124321332016 @default.
• W2112432133 countsByYear W21124321332017 @default.
• W2112432133 countsByYear W21124321332018 @default.
• W2112432133 countsByYear W21124321332019 @default.
• W2112432133 countsByYear W21124321332021 @default.
• W2112432133 countsByYear W21124321332022 @default.
• W2112432133 countsByYear W21124321332023 @default.
• W2112432133 crossrefType "journal-article" @default.
• W2112432133 hasAuthorship W2112432133A5010654465 @default.
• W2112432133 hasAuthorship W2112432133A5011580238 @default.
• W2112432133 hasAuthorship W2112432133A5016971703 @default.
• W2112432133 hasAuthorship W2112432133A5059203004 @default.
• W2112432133 hasConcept C12554922 @default.
• W2112432133 hasConcept C130316041 @default.
• W2112432133 hasConcept C153911025 @default.
• W2112432133 hasConcept C167508917 @default.
• W2112432133 hasConcept C185154212 @default.
• W2112432133 hasConcept C185592680 @default.
• W2112432133 hasConcept C195856716 @default.
• W2112432133 hasConcept C22744801 @default.
• W2112432133 hasConcept C2776330855 @default.
• W2112432133 hasConcept C2778918659 @default.
• W2112432133 hasConcept C2779564620 @default.
• W2112432133 hasConcept C2781170229 @default.
• W2112432133 hasConcept C41625074 @default.
• W2112432133 hasConcept C43617362 @default.
• W2112432133 hasConcept C552990157 @default.
• W2112432133 hasConcept C55493867 @default.
• W2112432133 hasConcept C86803240 @default.
• W2112432133 hasConceptScore W2112432133C12554922 @default.
• W2112432133 hasConceptScore W2112432133C130316041 @default.
• W2112432133 hasConceptScore W2112432133C153911025 @default.
• W2112432133 hasConceptScore W2112432133C167508917 @default.
• W2112432133 hasConceptScore W2112432133C185154212 @default.
• W2112432133 hasConceptScore W2112432133C185592680 @default.
• W2112432133 hasConceptScore W2112432133C195856716 @default.
• W2112432133 hasConceptScore W2112432133C22744801 @default.
• W2112432133 hasConceptScore W2112432133C2776330855 @default.
• W2112432133 hasConceptScore W2112432133C2778918659 @default.
• W2112432133 hasConceptScore W2112432133C2779564620 @default.
• W2112432133 hasConceptScore W2112432133C2781170229 @default.
• W2112432133 hasConceptScore W2112432133C41625074 @default.
• W2112432133 hasConceptScore W2112432133C43617362 @default.
• W2112432133 hasConceptScore W2112432133C552990157 @default.
• W2112432133 hasConceptScore W2112432133C55493867 @default.
• W2112432133 hasConceptScore W2112432133C86803240 @default.
• W2112432133 hasIssue "1-2" @default.
• W2112432133 hasLocation W21124321331 @default.
• W2112432133 hasLocation W21124321332 @default.
• W2112432133 hasOpenAccess W2112432133 @default.
• W2112432133 hasPrimaryLocation W21124321331 @default.
• W2112432133 hasRelatedWork W2072843686 @default.
• W2112432133 hasRelatedWork W2073396018 @default.
• W2112432133 hasRelatedWork W2090148718 @default.
• W2112432133 hasRelatedWork W2125148981 @default.
• W2112432133 hasRelatedWork W2244418172 @default.
• W2112432133 hasRelatedWork W2410967278 @default.
• W2112432133 hasRelatedWork W2413467403 @default.
• W2112432133 hasRelatedWork W2508104445 @default.
• W2112432133 hasRelatedWork W31346175 @default.
• W2112432133 hasRelatedWork W2166063086 @default.
• W2112432133 hasVolume "12" @default.
• W2112432133 isParatext "false" @default.
• W2112432133 isRetracted "false" @default.
• W2112432133 magId "2112432133" @default.

• next