Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112480384> ?p ?o ?g. }
Showing items 1 to 48 of
48
with 100 items per page.
- W2112480384 abstract "Abstract : This project focuses on the product of the HER2/Neu oncogene, a receptor tyrosine kinase that is amplified in 25-30% of human primary breast tumors. The overall goal of the project is to characterize the substrate specificity 0 one HER2/Neu kinase. In previous years, we expressed and purified HER2/Neu using the SfO,baculovirus system. We then used peptide library technology to isolate novel peptide substrates for HER/Neu. One of them, AAEEIYAARRG, is the best synthetic peptide substrate reported to date for HER2/Neu. In the final year of the project, we produced several potential peptide-based inhibitors for HER2/Neu by replacing the tyrosine residue in the substrate peptide with the following amino acid analogs: (1) p-L-carboxy-Phe; (2) p-D-carboxy-Phe; (3) tetrafluoro-L-Phe; (4) tetrafluoro-D-Phe; (5) 3,5-diiodo-L-Phe. We produced an additional peptide inhibitor by introducing p-L-carboxy-Phe into another peptide isolated from the library (EDKVDYRMHRRG) The inhibitory potencies of these compounds against purified HER2/Neu were in the millimolar range; these values were too high for in vivo Inhibition of HER2/Neu. Instead, we used a microinjection technique to measure the ability of the parent peptide AAEEIYAARRG to block EGF-induced membrane ruffling in a fibroblast model system. The peptide showed significant inhibition of the EGF effect." @default.
- W2112480384 created "2016-06-24" @default.
- W2112480384 creator A5050315454 @default.
- W2112480384 date "2000-12-01" @default.
- W2112480384 modified "2023-09-25" @default.
- W2112480384 title "Peptide-Based Inhibitors of Neu Tyrosine Kinase" @default.
- W2112480384 doi "https://doi.org/10.21236/ada392289" @default.
- W2112480384 hasPublicationYear "2000" @default.
- W2112480384 type Work @default.
- W2112480384 sameAs 2112480384 @default.
- W2112480384 citedByCount "0" @default.
- W2112480384 crossrefType "report" @default.
- W2112480384 hasAuthorship W2112480384A5050315454 @default.
- W2112480384 hasConcept C101544691 @default.
- W2112480384 hasConcept C170493617 @default.
- W2112480384 hasConcept C184235292 @default.
- W2112480384 hasConcept C185592680 @default.
- W2112480384 hasConcept C2779281246 @default.
- W2112480384 hasConcept C42362537 @default.
- W2112480384 hasConcept C55493867 @default.
- W2112480384 hasConcept C70721500 @default.
- W2112480384 hasConcept C86803240 @default.
- W2112480384 hasConceptScore W2112480384C101544691 @default.
- W2112480384 hasConceptScore W2112480384C170493617 @default.
- W2112480384 hasConceptScore W2112480384C184235292 @default.
- W2112480384 hasConceptScore W2112480384C185592680 @default.
- W2112480384 hasConceptScore W2112480384C2779281246 @default.
- W2112480384 hasConceptScore W2112480384C42362537 @default.
- W2112480384 hasConceptScore W2112480384C55493867 @default.
- W2112480384 hasConceptScore W2112480384C70721500 @default.
- W2112480384 hasConceptScore W2112480384C86803240 @default.
- W2112480384 hasLocation W21124803841 @default.
- W2112480384 hasOpenAccess W2112480384 @default.
- W2112480384 hasPrimaryLocation W21124803841 @default.
- W2112480384 hasRelatedWork W1973052457 @default.
- W2112480384 hasRelatedWork W2014933830 @default.
- W2112480384 hasRelatedWork W2023568039 @default.
- W2112480384 hasRelatedWork W2068955281 @default.
- W2112480384 hasRelatedWork W2138173746 @default.
- W2112480384 hasRelatedWork W2162634493 @default.
- W2112480384 hasRelatedWork W2233144399 @default.
- W2112480384 hasRelatedWork W3049482917 @default.
- W2112480384 hasRelatedWork W3092529624 @default.
- W2112480384 hasRelatedWork W4288050680 @default.
- W2112480384 isParatext "false" @default.
- W2112480384 isRetracted "false" @default.
- W2112480384 magId "2112480384" @default.
- W2112480384 workType "report" @default.