FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112562740> ?p ?o ?g. }

• W2112562740 endingPage "240" @default.
• W2112562740 startingPage "236" @default.
• W2112562740 abstract "ABSTRACT We developed a highly sensitive PCR method that enables the diagnosis and posttherapeutic follow-up of visceral leishmaniasis with patient blood. The PCR assay was thoroughly optimized by successive procedural refinements to increase its sensitivity and specificity. It was compared to in vitro cultivation as well as to direct examination of bone marrow and to serology. Two hundred thirty-seven patients presenting with clinical signs compatible with visceral leishmaniasis were included in the study. Thirty-six were diagnosed as having Mediterranean visceral leishmaniasis (MVL). Twenty-three of them, including 19 AIDS patients, were monitored during and after treatment over a period from 2 weeks to 3 years. Our PCR assay proved more sensitive than in vitro cultivation, direct examination, and serology for all patients. It is simple and can be adapted to routine hospital diagnostic procedures. For the primary diagnosis of MVL, the sensitivity of PCR versus that of cultivation was 97 versus 55% with peripheral blood and 100 versus 81% with bone marrow samples. Regarding posttherapeutic follow-up, overall, 48% of positive samples were detected by PCR only. Seven patients presented with a clinical relapse during the study; six relapses were detected at first by PCR only, sometimes a few weeks before the reappearance of signs or symptoms. We conclude that an optimized and well-mastered PCR assay with a peripheral blood sample is sufficient to provide a secure diagnosis for all immunocompromised patients and most immunocompetent patients. We also suggest systematic posttherapeutic monitoring by PCR with peripheral blood for immunocompromised patients." @default.
• W2112562740 created "2016-06-24" @default.
• W2112562740 creator A5002108176 @default.
• W2112562740 creator A5006318605 @default.
• W2112562740 creator A5019445691 @default.
• W2112562740 creator A5021991876 @default.
• W2112562740 creator A5027809731 @default.
• W2112562740 creator A5029233916 @default.
• W2112562740 creator A5070399152 @default.
• W2112562740 creator A5079454714 @default.
• W2112562740 date "2000-01-01" @default.
• W2112562740 modified "2023-09-27" @default.
• W2112562740 title "Optimized PCR Using Patient Blood Samples For Diagnosis and Follow-Up of Visceral Leishmaniasis, with Special Reference to AIDS Patients" @default.
• W2112562740 cites W1515006299 @default.
• W2112562740 cites W1640348682 @default.
• W2112562740 cites W1772540997 @default.
• W2112562740 cites W1963735570 @default.
• W2112562740 cites W1975302457 @default.
• W2112562740 cites W1976500359 @default.
• W2112562740 cites W1983911349 @default.
• W2112562740 cites W1986117268 @default.
• W2112562740 cites W1986592654 @default.
• W2112562740 cites W1996192449 @default.
• W2112562740 cites W1999566349 @default.
• W2112562740 cites W2013825802 @default.
• W2112562740 cites W2014476907 @default.
• W2112562740 cites W2042689434 @default.
• W2112562740 cites W2042880479 @default.
• W2112562740 cites W2050717506 @default.
• W2112562740 cites W2072133989 @default.
• W2112562740 cites W2092899792 @default.
• W2112562740 cites W2104456097 @default.
• W2112562740 cites W2110650231 @default.
• W2112562740 cites W2121984803 @default.
• W2112562740 cites W2125161803 @default.
• W2112562740 cites W2130330182 @default.
• W2112562740 cites W2134253010 @default.
• W2112562740 cites W2148589496 @default.
• W2112562740 cites W2168467494 @default.
• W2112562740 cites W2168946125 @default.
• W2112562740 doi "https://doi.org/10.1128/jcm.38.1.236-240.2000" @default.
• W2112562740 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/88701" @default.
• W2112562740 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10618093" @default.
• W2112562740 hasPublicationYear "2000" @default.
• W2112562740 type Work @default.
• W2112562740 sameAs 2112562740 @default.
• W2112562740 citedByCount "143" @default.
• W2112562740 countsByYear W21125627402012 @default.
• W2112562740 countsByYear W21125627402013 @default.
• W2112562740 countsByYear W21125627402014 @default.
• W2112562740 countsByYear W21125627402015 @default.
• W2112562740 countsByYear W21125627402016 @default.
• W2112562740 countsByYear W21125627402017 @default.
• W2112562740 countsByYear W21125627402018 @default.
• W2112562740 countsByYear W21125627402019 @default.
• W2112562740 countsByYear W21125627402020 @default.
• W2112562740 countsByYear W21125627402021 @default.
• W2112562740 crossrefType "journal-article" @default.
• W2112562740 hasAuthorship W2112562740A5002108176 @default.
• W2112562740 hasAuthorship W2112562740A5006318605 @default.
• W2112562740 hasAuthorship W2112562740A5019445691 @default.
• W2112562740 hasAuthorship W2112562740A5021991876 @default.
• W2112562740 hasAuthorship W2112562740A5027809731 @default.
• W2112562740 hasAuthorship W2112562740A5029233916 @default.
• W2112562740 hasAuthorship W2112562740A5070399152 @default.
• W2112562740 hasAuthorship W2112562740A5079454714 @default.
• W2112562740 hasBestOaLocation W21125627402 @default.
• W2112562740 hasConcept C126322002 @default.
• W2112562740 hasConcept C141071460 @default.
• W2112562740 hasConcept C159654299 @default.
• W2112562740 hasConcept C203014093 @default.
• W2112562740 hasConcept C2776555147 @default.
• W2112562740 hasConcept C2778689377 @default.
• W2112562740 hasConcept C2780007613 @default.
• W2112562740 hasConcept C3017910909 @default.
• W2112562740 hasConcept C45189115 @default.
• W2112562740 hasConcept C71924100 @default.
• W2112562740 hasConcept C90924648 @default.
• W2112562740 hasConceptScore W2112562740C126322002 @default.
• W2112562740 hasConceptScore W2112562740C141071460 @default.
• W2112562740 hasConceptScore W2112562740C159654299 @default.
• W2112562740 hasConceptScore W2112562740C203014093 @default.
• W2112562740 hasConceptScore W2112562740C2776555147 @default.
• W2112562740 hasConceptScore W2112562740C2778689377 @default.
• W2112562740 hasConceptScore W2112562740C2780007613 @default.
• W2112562740 hasConceptScore W2112562740C3017910909 @default.
• W2112562740 hasConceptScore W2112562740C45189115 @default.
• W2112562740 hasConceptScore W2112562740C71924100 @default.
• W2112562740 hasConceptScore W2112562740C90924648 @default.
• W2112562740 hasIssue "1" @default.
• W2112562740 hasLocation W21125627401 @default.
• W2112562740 hasLocation W21125627402 @default.
• W2112562740 hasLocation W21125627403 @default.
• W2112562740 hasOpenAccess W2112562740 @default.
• W2112562740 hasPrimaryLocation W21125627401 @default.
• W2112562740 hasRelatedWork W1772540997 @default.
• W2112562740 hasRelatedWork W1915544468 @default.
• W2112562740 hasRelatedWork W1934485756 @default.

• next