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• W2112567583 endingPage "1810" @default.
• W2112567583 startingPage "1801" @default.
• W2112567583 abstract "A G----T mutation at the start-point of transcription of the phage P22 sar promoter (sar + 1T) causes a novel defect in promoter clearance by Escherichia coli RNA polymerase (RNAP) in vitro. Under standard transcription conditions, in the presence of high concentrations of all four NTPs, the predominant products from this promoter are poly(U) chains of varying length. Because the mutation creates a run of four T: A base-pairs from - 1 to +3 (TGTT----TTTT), we propose that synthesis of poly(U) is pseudo-templated by the A4 stretch on the template strand. G----A and G----C mutations at position +1 do not cause pseudo-templated transcription. Several molecules of poly(U) are produced and released per sar+1T promoter-polymerase complex without dissociation of RNAP from the template DNA. The exponential relationship between yield and size of individual poly(U) species indicates that there is a constant probability that another U residue will be added to the nascent chain. Presumably, pseudo-templated transcription occurs by a slippage (stuttering) mechanism like that proposed to explain certain kinds of RNA editing in eukaryotic viral mRNAs." @default.
• W2112567583 created "2016-06-24" @default.
• W2112567583 creator A5078179170 @default.
• W2112567583 creator A5007087508 @default.
• W2112567583 date "1990-10-01" @default.
• W2112567583 modified "2023-10-16" @default.
• W2112567583 title "Pseudo-templated transcription by Escherichia coli RNA polymerase at a mutant promoter." @default.
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• W2112567583 doi "https://doi.org/10.1101/gad.4.10.1801" @default.
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