FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112587413> ?p ?o ?g. }

• W2112587413 endingPage "4799" @default.
• W2112587413 startingPage "4791" @default.
• W2112587413 abstract "ABSTRACT RstBA, a two-component regulatory system of Escherichia coli with an unidentified regulatory function, is under the control of a Mg 2+ -sensing PhoQP two-component system. In order to identify the network of transcription regulation downstream of RstBA, we isolated a set of RstA-binding sequences from the E. coli genome by using the genomic SELEX system. A gel mobility shift assay indicated the binding of RstA to two SELEX DNA fragments, one including the promoter region of asr (acid shock RNA) and another including the promoter for csgD (a regulator of the curli operon). Using a DNase I footprinting assay, we determined the RstA-binding sites (RstA boxes) with the consensus sequence TACATNTNGTTACA. Transcription of the asr gene was induced 10- to 60-fold either in low-pH (pH 4.5) LB medium or in low-phosphate minimal medium as detected by promoter assay. The acid-induced in vivo transcription of asr was reduced after the deletion of rstA . In vivo transcription of the asr promoter was observed only in the presence of RstA. In agreement with the PhoQP-RstBA network, the addition of Mg 2+ led to a severe reduction of the asr promoter activity, and the disruption of phoP also reduced the asr promoter activity, albeit to a lesser extent. These observations altogether indicate that RstA is an activator of asr transcription. In contrast, transcription of csgD was repressed by overexpression of RstA, indicating that RstA is a repressor for csgD . With these data taken together, we conclude that the expression of both asr and csgD is under the direct control of the PhoQP-RstBA signal relay cascade." @default.
• W2112587413 created "2016-06-24" @default.
• W2112587413 creator A5010271995 @default.
• W2112587413 creator A5036345766 @default.
• W2112587413 creator A5053367975 @default.
• W2112587413 creator A5061595177 @default.
• W2112587413 creator A5081836698 @default.
• W2112587413 creator A5083146431 @default.
• W2112587413 date "2007-07-01" @default.
• W2112587413 modified "2023-09-24" @default.
• W2112587413 title "Genomic SELEX Search for Target Promoters under the Control of the PhoQP-RstBA Signal Relay Cascade" @default.
• W2112587413 cites W1491682838 @default.
• W2112587413 cites W1544093580 @default.
• W2112587413 cites W1579416801 @default.
• W2112587413 cites W170850678 @default.
• W2112587413 cites W1712380815 @default.
• W2112587413 cites W1880811828 @default.
• W2112587413 cites W1958241309 @default.
• W2112587413 cites W1961858939 @default.
• W2112587413 cites W1973660142 @default.
• W2112587413 cites W1981044688 @default.
• W2112587413 cites W1982830713 @default.
• W2112587413 cites W2022126733 @default.
• W2112587413 cites W2051196796 @default.
• W2112587413 cites W2051449352 @default.
• W2112587413 cites W2063822718 @default.
• W2112587413 cites W2066933443 @default.
• W2112587413 cites W2071854990 @default.
• W2112587413 cites W2081929574 @default.
• W2112587413 cites W2084381419 @default.
• W2112587413 cites W2093262908 @default.
• W2112587413 cites W2093286425 @default.
• W2112587413 cites W2100122962 @default.
• W2112587413 cites W2105143878 @default.
• W2112587413 cites W2106952187 @default.
• W2112587413 cites W2108559568 @default.
• W2112587413 cites W2130342394 @default.
• W2112587413 cites W2138371117 @default.
• W2112587413 cites W2142193549 @default.
• W2112587413 cites W2147776446 @default.
• W2112587413 cites W2151387894 @default.
• W2112587413 cites W2155310856 @default.
• W2112587413 cites W2155955109 @default.
• W2112587413 cites W2158279787 @default.
• W2112587413 cites W2158345344 @default.
• W2112587413 cites W2159177223 @default.
• W2112587413 cites W2160084442 @default.
• W2112587413 cites W2160170449 @default.
• W2112587413 cites W2164806933 @default.
• W2112587413 cites W2167708455 @default.
• W2112587413 cites W2170158203 @default.
• W2112587413 doi "https://doi.org/10.1128/jb.00319-07" @default.
• W2112587413 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1913430" @default.
• W2112587413 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17468243" @default.
• W2112587413 hasPublicationYear "2007" @default.
• W2112587413 type Work @default.
• W2112587413 sameAs 2112587413 @default.
• W2112587413 citedByCount "119" @default.
• W2112587413 countsByYear W21125874132012 @default.
• W2112587413 countsByYear W21125874132013 @default.
• W2112587413 countsByYear W21125874132014 @default.
• W2112587413 countsByYear W21125874132015 @default.
• W2112587413 countsByYear W21125874132016 @default.
• W2112587413 countsByYear W21125874132017 @default.
• W2112587413 countsByYear W21125874132018 @default.
• W2112587413 countsByYear W21125874132019 @default.
• W2112587413 countsByYear W21125874132020 @default.
• W2112587413 countsByYear W21125874132021 @default.
• W2112587413 countsByYear W21125874132022 @default.
• W2112587413 countsByYear W21125874132023 @default.
• W2112587413 crossrefType "journal-article" @default.
• W2112587413 hasAuthorship W2112587413A5010271995 @default.
• W2112587413 hasAuthorship W2112587413A5036345766 @default.
• W2112587413 hasAuthorship W2112587413A5053367975 @default.
• W2112587413 hasAuthorship W2112587413A5061595177 @default.
• W2112587413 hasAuthorship W2112587413A5081836698 @default.
• W2112587413 hasAuthorship W2112587413A5083146431 @default.
• W2112587413 hasBestOaLocation W21125874132 @default.
• W2112587413 hasConcept C101762097 @default.
• W2112587413 hasConcept C104317684 @default.
• W2112587413 hasConcept C122682173 @default.
• W2112587413 hasConcept C138885662 @default.
• W2112587413 hasConcept C150194340 @default.
• W2112587413 hasConcept C153911025 @default.
• W2112587413 hasConcept C158448853 @default.
• W2112587413 hasConcept C179926584 @default.
• W2112587413 hasConcept C203075996 @default.
• W2112587413 hasConcept C22427896 @default.
• W2112587413 hasConcept C2778021871 @default.
• W2112587413 hasConcept C41895202 @default.
• W2112587413 hasConcept C54355233 @default.
• W2112587413 hasConcept C547475151 @default.
• W2112587413 hasConcept C67705224 @default.
• W2112587413 hasConcept C86339819 @default.
• W2112587413 hasConcept C86803240 @default.
• W2112587413 hasConcept C88045685 @default.
• W2112587413 hasConceptScore W2112587413C101762097 @default.
• W2112587413 hasConceptScore W2112587413C104317684 @default.

• next