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• W2112742336 startingPage "89" @default.
• W2112742336 abstract "Intact wild-type tomato (Lycopersicon esculentum cv. Moneymaker) seeds do not complete germination to the same percentage or at the same speed as intact ABA-deficient sitiens (sitw) mutant seeds when seeds of both genotypes are imbibed on polyethylene glycol (PEG) solutions of −0.3 to −1.5 MPa osmotic potential. However, if the thicker testas of wild-type seeds are removed (stripped) from the micropyle without damaging the endosperm, both the percentage and speed of germination at low external water potential are similar to that of sitw mutant seeds. Removing the micropylar end of the testa from sitw seeds did not enhance either the speed or percentage of germination on PEG solution. Despite similar germination percentage and speed between stripped wild-type seeds and either stripped or intact sitw seeds under osmotic stress, some differences in seed metabolism are evident between genotypes. The activity of endo-β-mannanase was greater in the endosperm of sitw mutant seeds compared to the endosperm of wild-type seeds when seeds were exposed to osmotic stress. Although α-galactosidase activities were similar between the two genotypes, monosaccharide, sucrose and raffinose contents were greater in the sitw mutant embryos than the wild-type embryos when tomato seeds were exposed to −0.3 MPa external water potentials. Neutral sugar contents actually increased above the amounts of these sugars in 1 h imbibed seeds during germination on −0.3 MPa PEG solution. However, neither greater endo-β-mannanase activity nor greater amounts of sugar in the sitw seeds result in significant differences in germination parameters when stripped wild-type and stripped or intact sitw seeds were germinated on PEG solutions of −0.3 MPa. The ability of intact, ABA-deficient sitw mutant tomato seeds to complete germination at much lower external osmotic conditions relative to the intact wild-type Moneymaker cultivar was solely dependent on a structural alteration in the mutant testa, making it more delicate and lesssening its resistance to penetration by the radicle." @default.
• W2112742336 created "2016-06-24" @default.
• W2112742336 creator A5049717755 @default.
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• W2112742336 date "1996-01-01" @default.
• W2112742336 modified "2023-09-27" @default.
• W2112742336 title "Primary dormancy in tomato (<i>Lycopersicon esculentum</i>cv. Moneymaker): studies with the<i>sitiens</i>mutant" @default.
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• W2112742336 doi "https://doi.org/10.1093/jxb/47.1.89" @default.
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