FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112769897> ?p ?o ?g. }

• W2112769897 endingPage "1474" @default.
• W2112769897 startingPage "1463" @default.
• W2112769897 abstract "The dbl oncogene encodes a prototype member of the Rho GTPase guanine nucleotide exchange factor (GEF) family. Oncogenic activation of proto-Dbl occurs through truncation of the N-terminal 497 residues. The C-terminal half of proto-Dbl includes residues 498 to 680 and 710 to 815, which fold into the Dbl homology (DH) domain and the pleckstrin homology (PH) domain, respectively, both of which are essential for cell transformation via the Rho GEF activity or cytoskeletal targeting function. Here we have investigated the mechanism of the apparent negative regulation of proto-Dbl imposed by the N-terminal sequences. Deletion of the N-terminal 285 or C-terminal 100 residues of proto-Dbl did not significantly affect either its transforming activity or GEF activity, while removal of the N-terminal 348 amino acids resulted in a significant increase in both transformation and GEF potential. Proto-Dbl displayed a mostly perinuclear distribution pattern, similar to a polypeptide derived from its N-terminal sequences, whereas onco-Dbl colocalized with actin stress fibers, like the PH domain. Coexpression of the N-terminal 482 residues with onco-Dbl resulted in disruption of its cytoskeletal localization and led to inhibition of onco-Dbl transforming activity. The apparent interference with the DH and PH functions by the N-terminal sequences can be rationalized by the observation that the N-terminal 482 residues or a fragment containing residues 286 to 482 binds specifically to the PH domain, limiting the access of Rho GTPases to the catalytic DH domain and masking the intracellular targeting function of the PH domain. Taken together, our findings unveiled an autoinhibitory mode of regulation of proto-Dbl that is mediated by the intramolecular interaction between its N-terminal sequences and PH domain, directly impacting both the GEF function and intracellular distribution." @default.
• W2112769897 created "2016-06-24" @default.
• W2112769897 creator A5020584779 @default.
• W2112769897 creator A5031793815 @default.
• W2112769897 creator A5044410528 @default.
• W2112769897 creator A5059915339 @default.
• W2112769897 creator A5067643729 @default.
• W2112769897 creator A5068117583 @default.
• W2112769897 date "2001-03-01" @default.
• W2112769897 modified "2023-10-16" @default.
• W2112769897 title "Autoinhibition Mechanism of Proto-Dbl" @default.
• W2112769897 cites W1484050792 @default.
• W2112769897 cites W1583486328 @default.
• W2112769897 cites W1824220821 @default.
• W2112769897 cites W1911465857 @default.
• W2112769897 cites W1966732663 @default.
• W2112769897 cites W1967691264 @default.
• W2112769897 cites W1968279859 @default.
• W2112769897 cites W1982186905 @default.
• W2112769897 cites W1982731850 @default.
• W2112769897 cites W1983657784 @default.
• W2112769897 cites W1989143807 @default.
• W2112769897 cites W19904141 @default.
• W2112769897 cites W1995531673 @default.
• W2112769897 cites W1996374693 @default.
• W2112769897 cites W1997293429 @default.
• W2112769897 cites W1999538252 @default.
• W2112769897 cites W2001234795 @default.
• W2112769897 cites W2004373826 @default.
• W2112769897 cites W2007727729 @default.
• W2112769897 cites W2013592169 @default.
• W2112769897 cites W2014070531 @default.
• W2112769897 cites W2015493603 @default.
• W2112769897 cites W2016708856 @default.
• W2112769897 cites W2019258251 @default.
• W2112769897 cites W2024619839 @default.
• W2112769897 cites W2030616863 @default.
• W2112769897 cites W2036887265 @default.
• W2112769897 cites W2037670359 @default.
• W2112769897 cites W2047994341 @default.
• W2112769897 cites W2059161447 @default.
• W2112769897 cites W2061126410 @default.
• W2112769897 cites W2061913305 @default.
• W2112769897 cites W2065033398 @default.
• W2112769897 cites W2066622533 @default.
• W2112769897 cites W2077792958 @default.
• W2112769897 cites W2081012321 @default.
• W2112769897 cites W2090778735 @default.
• W2112769897 cites W2096791952 @default.
• W2112769897 cites W2113144462 @default.
• W2112769897 cites W2116463110 @default.
• W2112769897 cites W2118833233 @default.
• W2112769897 cites W2133486542 @default.
• W2112769897 cites W2140882934 @default.
• W2112769897 cites W2143624084 @default.
• W2112769897 cites W2144554951 @default.
• W2112769897 cites W2144604382 @default.
• W2112769897 cites W2146055738 @default.
• W2112769897 cites W2155947794 @default.
• W2112769897 cites W2161340336 @default.
• W2112769897 cites W2162521796 @default.
• W2112769897 cites W2171432125 @default.
• W2112769897 cites W2314880902 @default.
• W2112769897 cites W2338254664 @default.
• W2112769897 cites W2339112266 @default.
• W2112769897 cites W41287429 @default.
• W2112769897 cites W6484253 @default.
• W2112769897 doi "https://doi.org/10.1128/mcb.21.5.1463-1474.2001" @default.
• W2112769897 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/86692" @default.
• W2112769897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11238883" @default.
• W2112769897 hasPublicationYear "2001" @default.
• W2112769897 type Work @default.
• W2112769897 sameAs 2112769897 @default.
• W2112769897 citedByCount "75" @default.
• W2112769897 countsByYear W21127698972012 @default.
• W2112769897 countsByYear W21127698972013 @default.
• W2112769897 countsByYear W21127698972014 @default.
• W2112769897 countsByYear W21127698972018 @default.
• W2112769897 countsByYear W21127698972020 @default.
• W2112769897 countsByYear W21127698972022 @default.
• W2112769897 countsByYear W21127698972023 @default.
• W2112769897 crossrefType "journal-article" @default.
• W2112769897 hasAuthorship W2112769897A5020584779 @default.
• W2112769897 hasAuthorship W2112769897A5031793815 @default.
• W2112769897 hasAuthorship W2112769897A5044410528 @default.
• W2112769897 hasAuthorship W2112769897A5059915339 @default.
• W2112769897 hasAuthorship W2112769897A5067643729 @default.
• W2112769897 hasAuthorship W2112769897A5068117583 @default.
• W2112769897 hasBestOaLocation W21127698972 @default.
• W2112769897 hasConcept C104317684 @default.
• W2112769897 hasConcept C126619667 @default.
• W2112769897 hasConcept C14053116 @default.
• W2112769897 hasConcept C142669718 @default.
• W2112769897 hasConcept C1491633281 @default.
• W2112769897 hasConcept C167625842 @default.
• W2112769897 hasConcept C207332259 @default.
• W2112769897 hasConcept C49658373 @default.
• W2112769897 hasConcept C515207424 @default.

• next