FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112866601> ?p ?o ?g. }

• W2112866601 endingPage "3539" @default.
• W2112866601 startingPage "3531" @default.
• W2112866601 abstract "The Arabidopsis FAE1 beta-ketoacyl-CoA synthase (FAE1 KCS) catalyzes the condensation of malonyl-CoA with long-chain acyl-CoAs. Sequence analysis of FAE1 KCS predicted that this condensing enzyme is anchored to a membrane by two adjacent N-terminal membrane-spanning domains. In order to characterize the FAE1 KCS and analyze its mechanism, FAE1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharomyces cerevisiae. The membrane-bound enzyme was then solubilized and purified to near homogeneity on a metal affinity column. Wild-type recombinant FAE1 KCS was active with several acyl-CoA substrates, with highest activity towards saturated and monounsaturated C16 and C18. In the absence of an acyl-CoA substrate, FAE1 KCS was unable to carry out decarboxylation of [3-(14)C]malonyl-CoA, indicating that it requires binding of the acyl-CoA for decarboxylation activity. Site-directed mutagenesis was carried out on the FAE1 KCS to assess if this condensing enzyme was mechanistically related to the well characterized soluble condensing enzymes of fatty acid and flavonoid syntheses. A C223A mutant enzyme lacking the acylation site was unable to carry out decarboxylation of malonyl-CoA even when 18:1-CoA was present. Mutational analyses of the conserved Asn424 and His391 residues indicated the importance of these residues for FAE1-KCS activity. The results presented here provide the initial analysis of the reaction mechanism for a membrane-bound condensing enzyme from any source and provide evidence for a mechanism similar to the soluble condensing enzymes." @default.
• W2112866601 created "2016-06-24" @default.
• W2112866601 creator A5038738198 @default.
• W2112866601 creator A5091647050 @default.
• W2112866601 date "2002-07-01" @default.
• W2112866601 modified "2023-10-16" @default.
• W2112866601 title "Engineering and mechanistic studies of the<i>Arabidopsis</i>FAE1 β-ketoacyl-CoA synthase, FAE1 KCS" @default.
• W2112866601 cites W1493623656 @default.
• W2112866601 cites W1580726364 @default.
• W2112866601 cites W1699689362 @default.
• W2112866601 cites W1848896918 @default.
• W2112866601 cites W1982257304 @default.
• W2112866601 cites W1993367005 @default.
• W2112866601 cites W2000225365 @default.
• W2112866601 cites W2003481405 @default.
• W2112866601 cites W2008287304 @default.
• W2112866601 cites W2010410445 @default.
• W2112866601 cites W2014984178 @default.
• W2112866601 cites W2021266593 @default.
• W2112866601 cites W2031258463 @default.
• W2112866601 cites W2031490243 @default.
• W2112866601 cites W2040078492 @default.
• W2112866601 cites W2041164194 @default.
• W2112866601 cites W2048162605 @default.
• W2112866601 cites W2052124872 @default.
• W2112866601 cites W2052577954 @default.
• W2112866601 cites W2060913542 @default.
• W2112866601 cites W2063231890 @default.
• W2112866601 cites W2087449923 @default.
• W2112866601 cites W2089111178 @default.
• W2112866601 cites W2100837269 @default.
• W2112866601 cites W2120701931 @default.
• W2112866601 cites W2125995189 @default.
• W2112866601 cites W2127297714 @default.
• W2112866601 cites W2135565608 @default.
• W2112866601 cites W2139047057 @default.
• W2112866601 cites W2140014285 @default.
• W2112866601 cites W2146118211 @default.
• W2112866601 cites W2149258924 @default.
• W2112866601 cites W2152458080 @default.
• W2112866601 cites W2222962673 @default.
• W2112866601 cites W4293247451 @default.
• W2112866601 cites W5985742 @default.
• W2112866601 cites W99433170 @default.
• W2112866601 doi "https://doi.org/10.1046/j.1432-1033.2002.03039.x" @default.
• W2112866601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12135493" @default.
• W2112866601 hasPublicationYear "2002" @default.
• W2112866601 type Work @default.
• W2112866601 sameAs 2112866601 @default.
• W2112866601 citedByCount "66" @default.
• W2112866601 countsByYear W21128666012012 @default.
• W2112866601 countsByYear W21128666012013 @default.
• W2112866601 countsByYear W21128666012014 @default.
• W2112866601 countsByYear W21128666012015 @default.
• W2112866601 countsByYear W21128666012016 @default.
• W2112866601 countsByYear W21128666012017 @default.
• W2112866601 countsByYear W21128666012018 @default.
• W2112866601 countsByYear W21128666012019 @default.
• W2112866601 countsByYear W21128666012020 @default.
• W2112866601 countsByYear W21128666012021 @default.
• W2112866601 countsByYear W21128666012022 @default.
• W2112866601 crossrefType "journal-article" @default.
• W2112866601 hasAuthorship W2112866601A5038738198 @default.
• W2112866601 hasAuthorship W2112866601A5091647050 @default.
• W2112866601 hasConcept C104317684 @default.
• W2112866601 hasConcept C143065580 @default.
• W2112866601 hasConcept C161790260 @default.
• W2112866601 hasConcept C181199279 @default.
• W2112866601 hasConcept C185592680 @default.
• W2112866601 hasConcept C2777576037 @default.
• W2112866601 hasConcept C2779222958 @default.
• W2112866601 hasConcept C2779491563 @default.
• W2112866601 hasConcept C2779685201 @default.
• W2112866601 hasConcept C2780595602 @default.
• W2112866601 hasConcept C2910079589 @default.
• W2112866601 hasConcept C553450214 @default.
• W2112866601 hasConcept C55493867 @default.
• W2112866601 hasConceptScore W2112866601C104317684 @default.
• W2112866601 hasConceptScore W2112866601C143065580 @default.
• W2112866601 hasConceptScore W2112866601C161790260 @default.
• W2112866601 hasConceptScore W2112866601C181199279 @default.
• W2112866601 hasConceptScore W2112866601C185592680 @default.
• W2112866601 hasConceptScore W2112866601C2777576037 @default.
• W2112866601 hasConceptScore W2112866601C2779222958 @default.
• W2112866601 hasConceptScore W2112866601C2779491563 @default.
• W2112866601 hasConceptScore W2112866601C2779685201 @default.
• W2112866601 hasConceptScore W2112866601C2780595602 @default.
• W2112866601 hasConceptScore W2112866601C2910079589 @default.
• W2112866601 hasConceptScore W2112866601C553450214 @default.
• W2112866601 hasConceptScore W2112866601C55493867 @default.
• W2112866601 hasIssue "14" @default.
• W2112866601 hasLocation W21128666011 @default.
• W2112866601 hasLocation W21128666012 @default.
• W2112866601 hasOpenAccess W2112866601 @default.
• W2112866601 hasPrimaryLocation W21128666011 @default.
• W2112866601 hasRelatedWork W1494396563 @default.
• W2112866601 hasRelatedWork W1553943686 @default.
• W2112866601 hasRelatedWork W1649546940 @default.

• next