Matches in SemOpenAlex for { <https://semopenalex.org/work/W2112999567> ?p ?o ?g. }
- W2112999567 endingPage "2626" @default.
- W2112999567 startingPage "2620" @default.
- W2112999567 abstract "Thermophilic WF146 protease matures efficiently at 60 degrees C, but quite slowly at low temperatures. In this report, seven amino acid residues involved in interactions between the mature domain and the propeptide of the enzyme were substituted by corresponding residues of psychrophilic subtilisin S41 to generate mutant Mut7 (S105G/G107D/Y117E/S136N/V143G/K144E/D145S). Mut3 (S105G/G107D/Y117E) and Mut4 (S136N/V143G/K144E/D145S) were also constructed. Transferring structural features from S41 endowed Mut7 with a remarkably increased maturation rate, as well as an improved caseinolytic activity at 25 degrees C. Moreover, Mut3 and Mut4 each obtained one of the above endowments. Further studies suggest that low-temperature activity and maturation rate are not necessarily linked, and uncoupling structural elements modulating the two properties may be advantageous to cold adaptation." @default.
- W2112999567 created "2016-06-24" @default.
- W2112999567 creator A5008555321 @default.
- W2112999567 creator A5011995502 @default.
- W2112999567 creator A5018553233 @default.
- W2112999567 creator A5022484942 @default.
- W2112999567 creator A5050300826 @default.
- W2112999567 creator A5052893976 @default.
- W2112999567 creator A5057059703 @default.
- W2112999567 creator A5072750696 @default.
- W2112999567 date "2008-06-27" @default.
- W2112999567 modified "2023-10-17" @default.
- W2112999567 title "Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41" @default.
- W2112999567 cites W1969168425 @default.
- W2112999567 cites W1980435816 @default.
- W2112999567 cites W1982493879 @default.
- W2112999567 cites W1987878848 @default.
- W2112999567 cites W1990775158 @default.
- W2112999567 cites W1990878089 @default.
- W2112999567 cites W1996307860 @default.
- W2112999567 cites W1997354735 @default.
- W2112999567 cites W2008913755 @default.
- W2112999567 cites W2009680668 @default.
- W2112999567 cites W2013270869 @default.
- W2112999567 cites W2027734642 @default.
- W2112999567 cites W2028994826 @default.
- W2112999567 cites W2031409259 @default.
- W2112999567 cites W2034756360 @default.
- W2112999567 cites W2035246206 @default.
- W2112999567 cites W2037526639 @default.
- W2112999567 cites W2042308321 @default.
- W2112999567 cites W2048846412 @default.
- W2112999567 cites W2051455069 @default.
- W2112999567 cites W2052860476 @default.
- W2112999567 cites W2059278221 @default.
- W2112999567 cites W2060809301 @default.
- W2112999567 cites W2070603799 @default.
- W2112999567 cites W2083699890 @default.
- W2112999567 cites W2084911696 @default.
- W2112999567 cites W2097856428 @default.
- W2112999567 cites W2118321462 @default.
- W2112999567 cites W2140184260 @default.
- W2112999567 cites W2141948660 @default.
- W2112999567 cites W2149395069 @default.
- W2112999567 cites W2161281999 @default.
- W2112999567 cites W2168854517 @default.
- W2112999567 cites W2171130060 @default.
- W2112999567 cites W2226425375 @default.
- W2112999567 cites W4293247451 @default.
- W2112999567 doi "https://doi.org/10.1016/j.febslet.2008.06.041" @default.
- W2112999567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18586033" @default.
- W2112999567 hasPublicationYear "2008" @default.
- W2112999567 type Work @default.
- W2112999567 sameAs 2112999567 @default.
- W2112999567 citedByCount "12" @default.
- W2112999567 countsByYear W21129995672013 @default.
- W2112999567 countsByYear W21129995672015 @default.
- W2112999567 countsByYear W21129995672016 @default.
- W2112999567 countsByYear W21129995672017 @default.
- W2112999567 countsByYear W21129995672018 @default.
- W2112999567 countsByYear W21129995672021 @default.
- W2112999567 crossrefType "journal-article" @default.
- W2112999567 hasAuthorship W2112999567A5008555321 @default.
- W2112999567 hasAuthorship W2112999567A5011995502 @default.
- W2112999567 hasAuthorship W2112999567A5018553233 @default.
- W2112999567 hasAuthorship W2112999567A5022484942 @default.
- W2112999567 hasAuthorship W2112999567A5050300826 @default.
- W2112999567 hasAuthorship W2112999567A5052893976 @default.
- W2112999567 hasAuthorship W2112999567A5057059703 @default.
- W2112999567 hasAuthorship W2112999567A5072750696 @default.
- W2112999567 hasBestOaLocation W21129995671 @default.
- W2112999567 hasConcept C104317684 @default.
- W2112999567 hasConcept C143065580 @default.
- W2112999567 hasConcept C167625842 @default.
- W2112999567 hasConcept C181199279 @default.
- W2112999567 hasConcept C185592680 @default.
- W2112999567 hasConcept C22802001 @default.
- W2112999567 hasConcept C2776714187 @default.
- W2112999567 hasConcept C2779811187 @default.
- W2112999567 hasConcept C27911776 @default.
- W2112999567 hasConcept C2994376932 @default.
- W2112999567 hasConcept C515207424 @default.
- W2112999567 hasConcept C55493867 @default.
- W2112999567 hasConcept C7860815 @default.
- W2112999567 hasConcept C86803240 @default.
- W2112999567 hasConceptScore W2112999567C104317684 @default.
- W2112999567 hasConceptScore W2112999567C143065580 @default.
- W2112999567 hasConceptScore W2112999567C167625842 @default.
- W2112999567 hasConceptScore W2112999567C181199279 @default.
- W2112999567 hasConceptScore W2112999567C185592680 @default.
- W2112999567 hasConceptScore W2112999567C22802001 @default.
- W2112999567 hasConceptScore W2112999567C2776714187 @default.
- W2112999567 hasConceptScore W2112999567C2779811187 @default.
- W2112999567 hasConceptScore W2112999567C27911776 @default.
- W2112999567 hasConceptScore W2112999567C2994376932 @default.
- W2112999567 hasConceptScore W2112999567C515207424 @default.
- W2112999567 hasConceptScore W2112999567C55493867 @default.
- W2112999567 hasConceptScore W2112999567C7860815 @default.