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• W2113013257 abstract "S-Adenosyl-L-methionine (SAM) is the preferred cofactor for biological methyl group transfers to various substrates such as nucleic acids, proteins, and lipids. Here we present stereospecific (>95% of the desired enantiomer) and high-yield preparation of four fluorescent and biologically active SAM analogs and demonstrate their usefulness in binding studies. Using a fluorescence titration experiment, we obtained a K(d) of 0.38 microM for the S-2,6-diaminopurinylmethionine-SAM-III riboswitch complex." @default.
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• W2113013257 date "2010-01-01" @default.
• W2113013257 modified "2023-10-18" @default.
• W2113013257 title "Enzymatic stereospecific preparation of fluorescent S-adenosyl-l-methionine analogs" @default.
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• W2113013257 doi "https://doi.org/10.1016/j.ab.2009.09.013" @default.
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