Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113068862> ?p ?o ?g. }
- W2113068862 endingPage "4068" @default.
- W2113068862 startingPage "4060" @default.
- W2113068862 abstract "The human immunodeficiency virus type 1 (HIV-1) Pr55gag precursors were previously shown to assemble and bud efficiently as noninfectious virus-like particles (VLPs) when expressed in baculovirus-infected insect cells. In this study, we examined the abilities of foreign antigens to be incorporated on the outer surface of HIV-1 Gag particles. We have used a dual recombinant baculovirus, expressing the HIV-1 Gag gene and gD gene under the control of the P10 and polyhedrin promoters, respectively, to obtain hybrid VLPs. Transmission electron microscopy of insect cells infected with the dual recombinant revealed very large aggregates of particles budding from the cell membrane. The release of VLPs into the culture medium was clearly different for a recombinant baculovirus producing solely HIV-1 Gag, for which particles were uniformly distributed all around the cell surface. Biochemical analysis of hybrid particles indicated that glycoprotein gD was packaged into HIV-1 Gag VLPs. Moreover, the carboxy-terminal p6 region of Gag polyprotein and the glycoprotein gD intracytoplasmic domain were not required for gD incorporation. The experiments described here clearly demonstrate that glycoprotein gD can be packaged with HIV-1 Gag particles and released from insect cells." @default.
- W2113068862 created "2016-06-24" @default.
- W2113068862 creator A5037546466 @default.
- W2113068862 creator A5048904478 @default.
- W2113068862 creator A5050710816 @default.
- W2113068862 creator A5068913173 @default.
- W2113068862 creator A5072532313 @default.
- W2113068862 creator A5075559517 @default.
- W2113068862 creator A5081753613 @default.
- W2113068862 creator A5091224621 @default.
- W2113068862 date "1995-07-01" @default.
- W2113068862 modified "2023-09-23" @default.
- W2113068862 title "Incorporation of pseudorabies virus gD into human immunodeficiency virus type 1 Gag particles produced in baculovirus-infected cells" @default.
- W2113068862 cites W1508323061 @default.
- W2113068862 cites W1542639585 @default.
- W2113068862 cites W1557860493 @default.
- W2113068862 cites W1567879434 @default.
- W2113068862 cites W158060014 @default.
- W2113068862 cites W1597733532 @default.
- W2113068862 cites W1598935837 @default.
- W2113068862 cites W1623896444 @default.
- W2113068862 cites W1643517094 @default.
- W2113068862 cites W1766661391 @default.
- W2113068862 cites W1773718890 @default.
- W2113068862 cites W1774354581 @default.
- W2113068862 cites W1806998329 @default.
- W2113068862 cites W1824456479 @default.
- W2113068862 cites W1829655256 @default.
- W2113068862 cites W1908085580 @default.
- W2113068862 cites W1959432277 @default.
- W2113068862 cites W1969383215 @default.
- W2113068862 cites W1970630169 @default.
- W2113068862 cites W1977453669 @default.
- W2113068862 cites W1982218313 @default.
- W2113068862 cites W1985255412 @default.
- W2113068862 cites W1996103477 @default.
- W2113068862 cites W2008457983 @default.
- W2113068862 cites W2009482334 @default.
- W2113068862 cites W2009704023 @default.
- W2113068862 cites W2021080732 @default.
- W2113068862 cites W2023290813 @default.
- W2113068862 cites W2024764884 @default.
- W2113068862 cites W2025265412 @default.
- W2113068862 cites W2044312096 @default.
- W2113068862 cites W2047197893 @default.
- W2113068862 cites W2067369810 @default.
- W2113068862 cites W2072622802 @default.
- W2113068862 cites W2077748471 @default.
- W2113068862 cites W2089353735 @default.
- W2113068862 cites W2100837269 @default.
- W2113068862 cites W2109555964 @default.
- W2113068862 cites W2140613873 @default.
- W2113068862 cites W2147842371 @default.
- W2113068862 cites W2149403311 @default.
- W2113068862 cites W2152478016 @default.
- W2113068862 cites W2163409698 @default.
- W2113068862 cites W2172962204 @default.
- W2113068862 cites W2467781397 @default.
- W2113068862 doi "https://doi.org/10.1128/jvi.69.7.4060-4068.1995" @default.
- W2113068862 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/189140" @default.
- W2113068862 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7769663" @default.
- W2113068862 hasPublicationYear "1995" @default.
- W2113068862 type Work @default.
- W2113068862 sameAs 2113068862 @default.
- W2113068862 citedByCount "27" @default.
- W2113068862 countsByYear W21130688622013 @default.
- W2113068862 countsByYear W21130688622015 @default.
- W2113068862 countsByYear W21130688622021 @default.
- W2113068862 countsByYear W21130688622022 @default.
- W2113068862 countsByYear W21130688622023 @default.
- W2113068862 crossrefType "journal-article" @default.
- W2113068862 hasAuthorship W2113068862A5037546466 @default.
- W2113068862 hasAuthorship W2113068862A5048904478 @default.
- W2113068862 hasAuthorship W2113068862A5050710816 @default.
- W2113068862 hasAuthorship W2113068862A5068913173 @default.
- W2113068862 hasAuthorship W2113068862A5072532313 @default.
- W2113068862 hasAuthorship W2113068862A5075559517 @default.
- W2113068862 hasAuthorship W2113068862A5081753613 @default.
- W2113068862 hasAuthorship W2113068862A5091224621 @default.
- W2113068862 hasBestOaLocation W21130688621 @default.
- W2113068862 hasConcept C103878061 @default.
- W2113068862 hasConcept C104317684 @default.
- W2113068862 hasConcept C108625454 @default.
- W2113068862 hasConcept C153911025 @default.
- W2113068862 hasConcept C159047783 @default.
- W2113068862 hasConcept C2522874641 @default.
- W2113068862 hasConcept C2776387124 @default.
- W2113068862 hasConcept C2777212814 @default.
- W2113068862 hasConcept C2777773057 @default.
- W2113068862 hasConcept C2781140520 @default.
- W2113068862 hasConcept C39060996 @default.
- W2113068862 hasConcept C40767141 @default.
- W2113068862 hasConcept C54355233 @default.
- W2113068862 hasConcept C55493867 @default.
- W2113068862 hasConcept C6881719 @default.
- W2113068862 hasConcept C81885089 @default.
- W2113068862 hasConcept C86803240 @default.
- W2113068862 hasConceptScore W2113068862C103878061 @default.