FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113120772> ?p ?o ?g. }

• W2113120772 endingPage "6037" @default.
• W2113120772 startingPage "6033" @default.
• W2113120772 abstract "The reduction of specific uridines to dihydrouridine is one of the most common modifications in tRNA. Increased levels of the dihydrouridine modification are associated with cancer. Dihydrouridine synthases (Dus) from different subfamilies selectively reduce distinct uridines, located at spatially unique positions of folded tRNA, into dihydrouridine. Because the catalytic center of all Dus enzymes is conserved, it is unclear how the same protein fold can be reprogrammed to ensure that nucleotides exposed at spatially distinct faces of tRNA can be accommodated in the same active site. We show that the Escherichia coli DusC is specific toward U16 of tRNA. Unexpectedly, crystal structures of DusC complexes with tRNA(Phe) and tRNA(Trp) show that Dus subfamilies that selectively modify U16 or U20 in tRNA adopt identical folds but bind their respective tRNA substrates in an almost reverse orientation that differs by a 160° rotation. The tRNA docking orientation appears to be guided by subfamily-specific clusters of amino acids (binding signatures) together with differences in the shape of the positively charged tRNA-binding surfaces. tRNA orientations are further constrained by positional differences between the C-terminal recognition domains. The exquisite substrate specificity of Dus enzymes is therefore controlled by a relatively simple mechanism involving major reorientation of the whole tRNA molecule. Such reprogramming of the enzymatic specificity appears to be a unique evolutionary solution for altering tRNA recognition by the same protein fold." @default.
• W2113120772 created "2016-06-24" @default.
• W2113120772 creator A5024108011 @default.
• W2113120772 creator A5028476493 @default.
• W2113120772 creator A5035240489 @default.
• W2113120772 creator A5043774543 @default.
• W2113120772 creator A5049108590 @default.
• W2113120772 creator A5052892484 @default.
• W2113120772 creator A5067940445 @default.
• W2113120772 creator A5070036056 @default.
• W2113120772 creator A5071820529 @default.
• W2113120772 creator A5085005519 @default.
• W2113120772 creator A5089924949 @default.
• W2113120772 date "2015-04-22" @default.
• W2113120772 modified "2023-10-17" @default.
• W2113120772 title "Major reorientation of tRNA substrates defines specificity of dihydrouridine synthases" @default.
• W2113120772 cites W1802135738 @default.
• W2113120772 cites W1923195462 @default.
• W2113120772 cites W1964634242 @default.
• W2113120772 cites W1968321573 @default.
• W2113120772 cites W1973290333 @default.
• W2113120772 cites W1977361720 @default.
• W2113120772 cites W1978077199 @default.
• W2113120772 cites W2009110345 @default.
• W2113120772 cites W2019832209 @default.
• W2113120772 cites W2022290835 @default.
• W2113120772 cites W2032407233 @default.
• W2113120772 cites W2038840577 @default.
• W2113120772 cites W2044064939 @default.
• W2113120772 cites W2047689300 @default.
• W2113120772 cites W2064367668 @default.
• W2113120772 cites W2072734582 @default.
• W2113120772 cites W2074858412 @default.
• W2113120772 cites W2079796553 @default.
• W2113120772 cites W2080928191 @default.
• W2113120772 cites W2082125720 @default.
• W2113120772 cites W2087158203 @default.
• W2113120772 cites W2094729320 @default.
• W2113120772 cites W2098257366 @default.
• W2113120772 cites W2100353926 @default.
• W2113120772 cites W2108376871 @default.
• W2113120772 cites W2109108777 @default.
• W2113120772 cites W2111482169 @default.
• W2113120772 cites W2112643823 @default.
• W2113120772 cites W2114478647 @default.
• W2113120772 cites W2124026197 @default.
• W2113120772 cites W2131350133 @default.
• W2113120772 cites W2143815085 @default.
• W2113120772 cites W2154208264 @default.
• W2113120772 cites W2157548679 @default.
• W2113120772 cites W2163341755 @default.
• W2113120772 cites W2163482302 @default.
• W2113120772 doi "https://doi.org/10.1073/pnas.1500161112" @default.
• W2113120772 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4434734" @default.
• W2113120772 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25902496" @default.
• W2113120772 hasPublicationYear "2015" @default.
• W2113120772 type Work @default.
• W2113120772 sameAs 2113120772 @default.
• W2113120772 citedByCount "34" @default.
• W2113120772 countsByYear W21131207722015 @default.
• W2113120772 countsByYear W21131207722016 @default.
• W2113120772 countsByYear W21131207722017 @default.
• W2113120772 countsByYear W21131207722018 @default.
• W2113120772 countsByYear W21131207722019 @default.
• W2113120772 countsByYear W21131207722020 @default.
• W2113120772 countsByYear W21131207722021 @default.
• W2113120772 countsByYear W21131207722022 @default.
• W2113120772 countsByYear W21131207722023 @default.
• W2113120772 crossrefType "journal-article" @default.
• W2113120772 hasAuthorship W2113120772A5024108011 @default.
• W2113120772 hasAuthorship W2113120772A5028476493 @default.
• W2113120772 hasAuthorship W2113120772A5035240489 @default.
• W2113120772 hasAuthorship W2113120772A5043774543 @default.
• W2113120772 hasAuthorship W2113120772A5049108590 @default.
• W2113120772 hasAuthorship W2113120772A5052892484 @default.
• W2113120772 hasAuthorship W2113120772A5067940445 @default.
• W2113120772 hasAuthorship W2113120772A5070036056 @default.
• W2113120772 hasAuthorship W2113120772A5071820529 @default.
• W2113120772 hasAuthorship W2113120772A5085005519 @default.
• W2113120772 hasAuthorship W2113120772A5089924949 @default.
• W2113120772 hasBestOaLocation W21131207721 @default.
• W2113120772 hasConcept C104317684 @default.
• W2113120772 hasConcept C153957851 @default.
• W2113120772 hasConcept C181199279 @default.
• W2113120772 hasConcept C185592680 @default.
• W2113120772 hasConcept C50929876 @default.
• W2113120772 hasConcept C512185932 @default.
• W2113120772 hasConcept C55493867 @default.
• W2113120772 hasConcept C67705224 @default.
• W2113120772 hasConcept C71240020 @default.
• W2113120772 hasConcept C86803240 @default.
• W2113120772 hasConceptScore W2113120772C104317684 @default.
• W2113120772 hasConceptScore W2113120772C153957851 @default.
• W2113120772 hasConceptScore W2113120772C181199279 @default.
• W2113120772 hasConceptScore W2113120772C185592680 @default.
• W2113120772 hasConceptScore W2113120772C50929876 @default.
• W2113120772 hasConceptScore W2113120772C512185932 @default.
• W2113120772 hasConceptScore W2113120772C55493867 @default.

• next