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- W2113162422 abstract "An essential element of the reclamation strategy proposed by the oil sands mining industry in northern Alberta, Canada, includes the creation of wetlands for the bioremediation of mining waste materials. The mining process used to extract oil from these deposits results in the production of large volumes of process-affected water (OSPW) and sediments (OSPS), which must be incorporated into wetlands as a component of the reclaimed landscapes. Wood frogs (Rana sylvatica) are an abundant native species that might be expected to inhabit these reclaimed wetlands. The objective of this study was to determine potential detrimental effects of OSPW and OSPS on the growth and development of wood frogs. Several morphological (weight, length, condition factor) and biochemical (whole body tadpole thyroid hormone and triglyceride concentrations and metamorph hepatic glycogen concentration) endpoints were assessed in conjunction with hatchability and survivability of wood frog eggs and tadpoles exposed to process-affected materials (OSPM) under field and laboratory conditions. As part of this study, assay techniques were optimized to enable simultaneous measurement of whole body 3,5,3’-triiodothyronine (T3), thyroxine (T4) and triglyceride (TG) concentrations in wood frog tadpoles. These assays were used to monitor changes in T3, T4 and TG in wood frog tadpoles during development from hatching to metamorphosis (Gosner stages 19-46), to establish baseline levels for subsequent application of the assays to evaluate contaminant effects. The results indicated peak T3 and T4 concentrations occurred during metamorphic climax (Gosner stages 40-46) and prometamorphosis (Gosner stages 31-40), respectively. Maximal TG concentrations were also observed during prometamorphosis. These assays were further employed to assess body condition and" @default.
- W2113162422 created "2016-06-24" @default.
- W2113162422 creator A5057244862 @default.
- W2113162422 date "2009-01-01" @default.
- W2113162422 modified "2023-09-26" @default.
- W2113162422 title "Effects of Oil Sands Process-Affected Water and Substrates on Wood Frog (Rana sylvatica) Eggs and Tadpoles" @default.
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