FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113189847> ?p ?o ?g. }

• W2113189847 endingPage "1775" @default.
• W2113189847 startingPage "1761" @default.
• W2113189847 abstract "We have evaluated the utility of the hepatoma-derived hybrid cell line, WIF-B, for in vitro studies of polarized hepatocyte functions. The majority (&gt; 70%) of cells in confluent culture formed closed spaces with adjacent cells. These bile canalicular-like spaces (BC) accumulated fluorescein, a property of bile canaliculi in vivo. By indirect immunofluorescence, six plasma membrane (PM) proteins showed polarized distributions similar to rat hepatocytes in situ. Four apical PM proteins were concentrated in the BC membrane of WIF-B cells. Microtubules radiated from the BC (apical) membrane, and actin and foci of gamma-tubulin were concentrated in this region. The tight junction-associated protein ZO-1 was present in belts marking the boundary between apical and basolateral PM domains. We explored the functional properties of this boundary in living cells using fluorescent membrane lipid analogs and soluble tracers. When cells were incubated at 4 degrees C with a fluorescent analog of sphingomyelin, only the basolateral PM was labeled. In contrast, when both PM domains were labeled by de novo synthesis of fluorescent sphingomyelin from ceramide, fluorescent lipid could only be removed from the basolateral domain. These data demonstrate the presence of a barrier to the lateral diffusion of lipids between the PM domains. However, small soluble FITC-dextrans (4,400 mol wt) were able to diffuse into BC, while larger FITC-dextrans were restricted to various degrees depending on their size and incubation temperature. At 4 degrees C, the surface labeling reagent sNHS-LC-biotin (557 mol wt) had access to the entire PM, but streptavidin (60,000 mol wt), which binds to biotinylated molecules, was restricted to only the basolateral domain. Such differential accessibility of well-characterized probes can be used to mark each membrane domain separately. These results show that WIF-B cells are a suitable model to study membrane trafficking and targeting in hepatocytes in vitro." @default.
• W2113189847 created "2016-06-24" @default.
• W2113189847 creator A5010864874 @default.
• W2113189847 creator A5025773863 @default.
• W2113189847 creator A5029424185 @default.
• W2113189847 creator A5059359203 @default.
• W2113189847 creator A5066037259 @default.
• W2113189847 creator A5070770210 @default.
• W2113189847 creator A5077359342 @default.
• W2113189847 creator A5087094437 @default.
• W2113189847 creator A5090108427 @default.
• W2113189847 date "1993-12-15" @default.
• W2113189847 modified "2023-10-10" @default.
• W2113189847 title "WIF-B cells: an in vitro model for studies of hepatocyte polarity." @default.
• W2113189847 cites W1487490391 @default.
• W2113189847 cites W1492692834 @default.
• W2113189847 cites W1538065055 @default.
• W2113189847 cites W1602063632 @default.
• W2113189847 cites W1620145154 @default.
• W2113189847 cites W1646404713 @default.
• W2113189847 cites W1844745998 @default.
• W2113189847 cites W1888874925 @default.
• W2113189847 cites W1899335257 @default.
• W2113189847 cites W1970389055 @default.
• W2113189847 cites W1974114737 @default.
• W2113189847 cites W1978462371 @default.
• W2113189847 cites W1979991124 @default.
• W2113189847 cites W1980181948 @default.
• W2113189847 cites W1981305491 @default.
• W2113189847 cites W1981345384 @default.
• W2113189847 cites W1997986612 @default.
• W2113189847 cites W2006698894 @default.
• W2113189847 cites W2012051994 @default.
• W2113189847 cites W2012386903 @default.
• W2113189847 cites W2017145860 @default.
• W2113189847 cites W2018371100 @default.
• W2113189847 cites W2026549297 @default.
• W2113189847 cites W2027899810 @default.
• W2113189847 cites W2032920533 @default.
• W2113189847 cites W2033306271 @default.
• W2113189847 cites W2041433461 @default.
• W2113189847 cites W2041640512 @default.
• W2113189847 cites W2045851431 @default.
• W2113189847 cites W2046560218 @default.
• W2113189847 cites W2050421082 @default.
• W2113189847 cites W2063391451 @default.
• W2113189847 cites W2066649831 @default.
• W2113189847 cites W2076889165 @default.
• W2113189847 cites W2085740119 @default.
• W2113189847 cites W2090461880 @default.
• W2113189847 cites W2090467384 @default.
• W2113189847 cites W2094091694 @default.
• W2113189847 cites W2098151972 @default.
• W2113189847 cites W2102967325 @default.
• W2113189847 cites W2105762153 @default.
• W2113189847 cites W2106508078 @default.
• W2113189847 cites W2111930154 @default.
• W2113189847 cites W2112551160 @default.
• W2113189847 cites W2125029933 @default.
• W2113189847 cites W2126979211 @default.
• W2113189847 cites W2128474008 @default.
• W2113189847 cites W2131649752 @default.
• W2113189847 cites W2134748481 @default.
• W2113189847 cites W2145621252 @default.
• W2113189847 cites W2148004059 @default.
• W2113189847 cites W2149202604 @default.
• W2113189847 cites W2155579605 @default.
• W2113189847 cites W2156766814 @default.
• W2113189847 cites W2165819323 @default.
• W2113189847 cites W2176544002 @default.
• W2113189847 cites W2186768487 @default.
• W2113189847 cites W224961340 @default.
• W2113189847 cites W2284013506 @default.
• W2113189847 cites W2300917877 @default.
• W2113189847 cites W2324949367 @default.
• W2113189847 cites W2410258515 @default.
• W2113189847 cites W2411398080 @default.
• W2113189847 cites W2415126165 @default.
• W2113189847 cites W2415174172 @default.
• W2113189847 cites W2417325128 @default.
• W2113189847 cites W2424341642 @default.
• W2113189847 cites W2425162369 @default.
• W2113189847 cites W2428265599 @default.
• W2113189847 cites W2440753934 @default.
• W2113189847 cites W2469261093 @default.
• W2113189847 cites W2469543426 @default.
• W2113189847 cites W287067515 @default.
• W2113189847 doi "https://doi.org/10.1083/jcb.123.6.1761" @default.
• W2113189847 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2290861" @default.
• W2113189847 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7506266" @default.
• W2113189847 hasPublicationYear "1993" @default.
• W2113189847 type Work @default.
• W2113189847 sameAs 2113189847 @default.
• W2113189847 citedByCount "198" @default.
• W2113189847 countsByYear W21131898472012 @default.
• W2113189847 countsByYear W21131898472013 @default.
• W2113189847 countsByYear W21131898472014 @default.
• W2113189847 countsByYear W21131898472015 @default.

• next