FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113192692> ?p ?o ?g. }

• W2113192692 endingPage "667" @default.
• W2113192692 startingPage "661" @default.
• W2113192692 abstract "The heterogeneity in bacterial surface macromolecules was probed by examining individual macromolecules on the surface of Pseudomonas putida KT2442 via single-molecule force spectroscopy (SMFS). Using an atomic force microscope (AFM), the silicon nitride tip was brought into contact with biopolymer molecules on bacterial cells and these macromolecules were stretched. Force-extension measurements on different bacterial cells showed a range of adhesion affinities and polymer lengths. However, substantial heterogeneity was also observed in the force-extension curves on a single bacterium. A given bacterium has biopolymers that range in size from tens to hundreds of nanometers, with adhesion affinities for the AFM tip from nearly zero to greater than 1 nN. A distribution of polymer sizes was confirmed by size-exclusion chromatography. The freely jointed chain (FJC) model for polymer elasticity was applied to individual force-extension curves in order to estimate the contour lengths and segment lengths of the polymer chains. A range of segment lengths was obtained using the FJC model, from 0.154-0.45 nm in water, 0.154-0.32 nm in 0.01 M KCl, and 0.154-0.65 nm in 0.1 M KCl. The modeling confirms that the heterogeneity in biopolymers is more than a matter of differences in molecular weights, since a range of stiffnesses (segment lengths) was also observed. The effect of salt concentration on biopolymer conformation and adhesion was also explored. While the biopolymers were flexible in all solvents, they were slightly more extended in water than in either of the salt solutions (0.01 and 0.1 M KCl). The adhesion of polysaccharides with the AFM tip was not dependent on salt concentration, because the polymers were not highly charged and heterogeneity overwhelmed any trends that could be observed in adhesion with respect to solution ionic strength. These experiments indicate that heterogeneity in biopolymer properties on an individual bacterium and within a population of bacterial cells may be much greater than previously believed and should be incorporated into models of bacterial adhesion." @default.
• W2113192692 created "2016-06-24" @default.
• W2113192692 creator A5015488458 @default.
• W2113192692 creator A5068498787 @default.
• W2113192692 date "2002-06-05" @default.
• W2113192692 modified "2023-10-01" @default.
• W2113192692 title "Heterogeneity in Bacterial Surface Polysaccharides, Probed on a Single-Molecule Basis" @default.
• W2113192692 cites W1516194903 @default.
• W2113192692 cites W1551658018 @default.
• W2113192692 cites W1569994690 @default.
• W2113192692 cites W1586966961 @default.
• W2113192692 cites W1659791802 @default.
• W2113192692 cites W1669551441 @default.
• W2113192692 cites W1966544491 @default.
• W2113192692 cites W1986661446 @default.
• W2113192692 cites W1989474839 @default.
• W2113192692 cites W1993412930 @default.
• W2113192692 cites W1998276013 @default.
• W2113192692 cites W2000610417 @default.
• W2113192692 cites W2001907809 @default.
• W2113192692 cites W2010423863 @default.
• W2113192692 cites W2015667791 @default.
• W2113192692 cites W2030732731 @default.
• W2113192692 cites W2033404326 @default.
• W2113192692 cites W2038411578 @default.
• W2113192692 cites W2047282728 @default.
• W2113192692 cites W2047397572 @default.
• W2113192692 cites W2047496476 @default.
• W2113192692 cites W2051107366 @default.
• W2113192692 cites W2053333865 @default.
• W2113192692 cites W2053787721 @default.
• W2113192692 cites W2054427689 @default.
• W2113192692 cites W2057979629 @default.
• W2113192692 cites W2058912578 @default.
• W2113192692 cites W2072706326 @default.
• W2113192692 cites W2072903858 @default.
• W2113192692 cites W2076501176 @default.
• W2113192692 cites W2081407528 @default.
• W2113192692 cites W2090777935 @default.
• W2113192692 cites W2118386239 @default.
• W2113192692 cites W2125955445 @default.
• W2113192692 cites W2137277059 @default.
• W2113192692 cites W2138774303 @default.
• W2113192692 cites W2144082864 @default.
• W2113192692 cites W2147521802 @default.
• W2113192692 cites W2153802117 @default.
• W2113192692 cites W2188199220 @default.
• W2113192692 doi "https://doi.org/10.1021/bm015648y" @default.
• W2113192692 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12099808" @default.
• W2113192692 hasPublicationYear "2002" @default.
• W2113192692 type Work @default.
• W2113192692 sameAs 2113192692 @default.
• W2113192692 citedByCount "119" @default.
• W2113192692 countsByYear W21131926922012 @default.
• W2113192692 countsByYear W21131926922013 @default.
• W2113192692 countsByYear W21131926922014 @default.
• W2113192692 countsByYear W21131926922015 @default.
• W2113192692 countsByYear W21131926922016 @default.
• W2113192692 countsByYear W21131926922017 @default.
• W2113192692 countsByYear W21131926922018 @default.
• W2113192692 countsByYear W21131926922019 @default.
• W2113192692 countsByYear W21131926922020 @default.
• W2113192692 countsByYear W21131926922021 @default.
• W2113192692 countsByYear W21131926922022 @default.
• W2113192692 countsByYear W21131926922023 @default.
• W2113192692 crossrefType "journal-article" @default.
• W2113192692 hasAuthorship W2113192692A5015488458 @default.
• W2113192692 hasAuthorship W2113192692A5068498787 @default.
• W2113192692 hasConcept C119473381 @default.
• W2113192692 hasConcept C121332964 @default.
• W2113192692 hasConcept C151827568 @default.
• W2113192692 hasConcept C171250308 @default.
• W2113192692 hasConcept C178790620 @default.
• W2113192692 hasConcept C185592680 @default.
• W2113192692 hasConcept C192562407 @default.
• W2113192692 hasConcept C204389451 @default.
• W2113192692 hasConcept C2778636629 @default.
• W2113192692 hasConcept C32909587 @default.
• W2113192692 hasConcept C521977710 @default.
• W2113192692 hasConcept C55493867 @default.
• W2113192692 hasConcept C57879066 @default.
• W2113192692 hasConcept C8010536 @default.
• W2113192692 hasConcept C84416704 @default.
• W2113192692 hasConcept C99895341 @default.
• W2113192692 hasConceptScore W2113192692C119473381 @default.
• W2113192692 hasConceptScore W2113192692C121332964 @default.
• W2113192692 hasConceptScore W2113192692C151827568 @default.
• W2113192692 hasConceptScore W2113192692C171250308 @default.
• W2113192692 hasConceptScore W2113192692C178790620 @default.
• W2113192692 hasConceptScore W2113192692C185592680 @default.
• W2113192692 hasConceptScore W2113192692C192562407 @default.
• W2113192692 hasConceptScore W2113192692C204389451 @default.
• W2113192692 hasConceptScore W2113192692C2778636629 @default.
• W2113192692 hasConceptScore W2113192692C32909587 @default.
• W2113192692 hasConceptScore W2113192692C521977710 @default.
• W2113192692 hasConceptScore W2113192692C55493867 @default.
• W2113192692 hasConceptScore W2113192692C57879066 @default.
• W2113192692 hasConceptScore W2113192692C8010536 @default.

• next