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- W2113195377 abstract "Abstract The Fc effector functions of immunoglobulin G (IgG) antibodies are in part determined by structural features of carbohydrates linked to each of the paired gamma heavy chains in the antibody constant domain (C H 2). One glycoform that has been shown to be advantageous is G2, where both arms of complex bi‐antennary N‐glycans terminate in galactose. In vitro treatment with glycosyltransferases can remodel heterogeneous IgG glycoforms, enabling preparation of IgG molecules with homogeneous glycan chains. Here we describe optimization of conditions for use of a soluble recombinant galactosyltransferase in vitro to remodel glycans of human serum IgG, and we demonstrate a scaled‐up reaction in which >98% of neutral glycans attached to 1 kg IgG are converted to the G2 glycoform. Removal of glycosylation reagents from the product is achieved in one step by affinity chromatography on immobilized Protein A. © 2005 Wiley Periodicals, Inc." @default.
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- W2113195377 date "2005-09-26" @default.
- W2113195377 modified "2023-10-15" @default.
- W2113195377 title "In vitro galactosylation of human IgG at 1 kg scale using recombinant galactosyltransferase" @default.
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- W2113195377 doi "https://doi.org/10.1002/bit.20658" @default.
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