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- W2113209979 abstract "Guanine quadruplex structures (GQSs) play important roles in the regulation of gene expression and cellular processes. Recent studies provide strong evidence for the formation and function of DNA and RNA GQSs in human cells. However, whether GQSs form and are functional in plants remains essentially unexplored. On the basis of circular dichroism (CD)-detected titration, UV-detected melting, in-line probing (ILP) and reporter gene assay studies, we report the first example of a plant RNA GQS that inhibits translation. This GQS is located within the 5′-UTR of the ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED (ATR) mRNA of Arabidopsis thaliana (mouse-ear cress). We show that this GQS is highly stable and is thermodynamically favoured over a competing hairpin structure in the 5′-UTR at physiological K+ and Mg2+ concentrations. Results from ILP reveal the secondary structure of the RNA and support formation of the GQS in vitro in the context of the complete 5′-UTR. Transient reporter gene assays performed in living plants reveal that the GQS inhibits translation but not transcription, implicating this GQS as a translational repressor in vivo. Our results provide the first complete demonstration of the formation and function of a regulatory RNA GQS in plants and open new avenues to explore potential functional roles of GQS in the plant kingdom." @default.
- W2113209979 created "2016-06-24" @default.
- W2113209979 creator A5017879021 @default.
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- W2113209979 creator A5054599523 @default.
- W2113209979 creator A5086117468 @default.
- W2113209979 date "2015-03-20" @default.
- W2113209979 modified "2023-09-27" @default.
- W2113209979 title "A stable RNA G-quadruplex within the 5′-UTR of <i>Arabidopsis thaliana ATR</i> mRNA inhibits translation" @default.
- W2113209979 cites W1488789106 @default.
- W2113209979 cites W1498439983 @default.
- W2113209979 cites W1512126598 @default.
- W2113209979 cites W1539503448 @default.
- W2113209979 cites W1964335041 @default.
- W2113209979 cites W1964578544 @default.
- W2113209979 cites W1965163574 @default.
- W2113209979 cites W1967630574 @default.
- W2113209979 cites W1975062656 @default.
- W2113209979 cites W1975998502 @default.
- W2113209979 cites W1985836645 @default.
- W2113209979 cites W1987919977 @default.
- W2113209979 cites W1988900456 @default.
- W2113209979 cites W1990635146 @default.
- W2113209979 cites W1993726540 @default.
- W2113209979 cites W1993752650 @default.
- W2113209979 cites W1993958212 @default.
- W2113209979 cites W1998846172 @default.
- W2113209979 cites W1998940503 @default.
- W2113209979 cites W2008090633 @default.
- W2113209979 cites W2013646237 @default.
- W2113209979 cites W2015508886 @default.
- W2113209979 cites W2020679328 @default.
- W2113209979 cites W2021191052 @default.
- W2113209979 cites W2022092530 @default.
- W2113209979 cites W2032698727 @default.
- W2113209979 cites W2036412199 @default.
- W2113209979 cites W2038304204 @default.
- W2113209979 cites W2039241171 @default.
- W2113209979 cites W2044132362 @default.
- W2113209979 cites W2045868610 @default.
- W2113209979 cites W2046567163 @default.
- W2113209979 cites W2047038417 @default.
- W2113209979 cites W2047210598 @default.
- W2113209979 cites W2049058499 @default.
- W2113209979 cites W2051558182 @default.
- W2113209979 cites W2056201880 @default.
- W2113209979 cites W2058920315 @default.
- W2113209979 cites W2065219073 @default.
- W2113209979 cites W2066018734 @default.
- W2113209979 cites W2066055007 @default.
- W2113209979 cites W2067022597 @default.
- W2113209979 cites W2071060330 @default.
- W2113209979 cites W2074613554 @default.
- W2113209979 cites W2076843089 @default.
- W2113209979 cites W2079768304 @default.
- W2113209979 cites W2088267546 @default.
- W2113209979 cites W2088710419 @default.
- W2113209979 cites W2091254099 @default.
- W2113209979 cites W2091876286 @default.
- W2113209979 cites W2104989176 @default.
- W2113209979 cites W2107875716 @default.
- W2113209979 cites W2108763751 @default.
- W2113209979 cites W2115053545 @default.
- W2113209979 cites W2119707666 @default.
- W2113209979 cites W2119879172 @default.
- W2113209979 cites W2122141896 @default.
- W2113209979 cites W2122846880 @default.
- W2113209979 cites W2124166350 @default.
- W2113209979 cites W2128345861 @default.
- W2113209979 cites W2129013431 @default.
- W2113209979 cites W2130652515 @default.
- W2113209979 cites W2131498592 @default.
- W2113209979 cites W2141157874 @default.
- W2113209979 cites W2141292707 @default.
- W2113209979 cites W2147863419 @default.
- W2113209979 cites W2153091158 @default.
- W2113209979 cites W2155773930 @default.
- W2113209979 cites W2157534860 @default.
- W2113209979 cites W2158777452 @default.
- W2113209979 cites W2162059428 @default.
- W2113209979 cites W2163533539 @default.
- W2113209979 cites W2165961683 @default.
- W2113209979 cites W2168605632 @default.
- W2113209979 cites W2169230340 @default.
- W2113209979 cites W2169745877 @default.
- W2113209979 cites W2169915963 @default.
- W2113209979 cites W2172154992 @default.
- W2113209979 cites W2315475605 @default.
- W2113209979 cites W2315967566 @default.
- W2113209979 cites W2331349367 @default.
- W2113209979 cites W2331917893 @default.
- W2113209979 cites W2334054197 @default.
- W2113209979 cites W2398906547 @default.
- W2113209979 cites W2502626196 @default.
- W2113209979 doi "https://doi.org/10.1042/bj20141063" @default.
- W2113209979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25793418" @default.
- W2113209979 hasPublicationYear "2015" @default.