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• W2113231870 abstract "Abstract The family of conserved colicin DNases E2, E7, E8, and E9 are microbial toxins that kill bacteria through random degradation of the chromosomal DNA. In the present work, we compare side by side the conformational stabilities of these four highly homologous colicin DNases. Our results indicate that the apo‐forms of these colicins are at room temperature and neutral pH in a dynamic conformational equilibrium between at least two quite distinct conformers. We show that the thermal stabilities of the apo‐proteins differ by up to 20°C. The observed differences correlate with the observed conformational behavior, that is, the tendency of the protein to form either an open, less stable or closed, more stable conformation in solution, as deduced by both tryptophan accessibility studies and electrospray ionization mass spectrometry. Given these surprising structural differences, we next probed the catalytic activity of the four DNases and also observed a significant variation in relative activities. However, no unequivocal link between the activity of the protein and its thermal and structural stability could easily be made. The observed differences in conformational and functional properties of the four colicin DNases are surprising given that they are a closely related (≥65% identity) family of enzymes containing a highly conserved (ββα‐Me) active site motif. The different behavior of the apo‐enzymes must therefore most likely depend on more subtle changes in amino acid sequences, most likely in the exosite region (residues 72–98) that is required for specific high‐affinity binding of the cognate immunity protein." @default.
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• W2113231870 date "2004-05-01" @default.
• W2113231870 modified "2023-09-29" @default.
• W2113231870 title "Distinct conformational stability and functional activity of four highly homologous endonuclease colicins" @default.
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• W2113231870 doi "https://doi.org/10.1110/ps.03508204" @default.
• W2113231870 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2286750" @default.
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• W2113231870 hasPublicationYear "2004" @default.
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