FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113231874> ?p ?o ?g. }

• W2113231874 endingPage "20652" @default.
• W2113231874 startingPage "20647" @default.
• W2113231874 abstract "Nanopores hold great promise as single-molecule analytical devices and biophysical model systems because the ionic current blockades they produce contain information about the identity, concentration, structure, and dynamics of target molecules. The porin MspA of Mycobacterium smegmatis has remarkable stability against environmental stresses and can be rationally modified based on its crystal structure. Further, MspA has a short and narrow channel constriction that is promising for DNA sequencing because it may enable improved characterization of short segments of a ssDNA molecule that is threaded through the pore. By eliminating the negative charge in the channel constriction, we designed and constructed an MspA mutant capable of electronically detecting and characterizing single molecules of ssDNA as they are electrophoretically driven through the pore. A second mutant with additional exchanges of negatively-charged residues for positively-charged residues in the vestibule region exhibited a factor of ≈20 higher interaction rates, required only half as much voltage to observe interaction, and allowed ssDNA to reside in the vestibule ≈100 times longer than the first mutant. Our results introduce MspA as a nanopore for nucleic acid analysis and highlight its potential as an engineerable platform for single-molecule detection and characterization applications." @default.
• W2113231874 created "2016-06-24" @default.
• W2113231874 creator A5018497969 @default.
• W2113231874 creator A5019834279 @default.
• W2113231874 creator A5036527909 @default.
• W2113231874 creator A5062657766 @default.
• W2113231874 creator A5085987918 @default.
• W2113231874 date "2008-12-30" @default.
• W2113231874 modified "2023-10-10" @default.
• W2113231874 title "Single-molecule DNA detection with an engineered MspA protein nanopore" @default.
• W2113231874 cites W1481296411 @default.
• W2113231874 cites W1585073473 @default.
• W2113231874 cites W1971212710 @default.
• W2113231874 cites W1978941223 @default.
• W2113231874 cites W1986553396 @default.
• W2113231874 cites W1994660369 @default.
• W2113231874 cites W1997740557 @default.
• W2113231874 cites W2005253245 @default.
• W2113231874 cites W2020002901 @default.
• W2113231874 cites W2020989750 @default.
• W2113231874 cites W2030683115 @default.
• W2113231874 cites W2032654772 @default.
• W2113231874 cites W2056386145 @default.
• W2113231874 cites W2057448845 @default.
• W2113231874 cites W2061446852 @default.
• W2113231874 cites W2073975746 @default.
• W2113231874 cites W2075777353 @default.
• W2113231874 cites W2095467724 @default.
• W2113231874 cites W2095898308 @default.
• W2113231874 cites W2098309035 @default.
• W2113231874 cites W2099446866 @default.
• W2113231874 cites W2122279724 @default.
• W2113231874 cites W2123328412 @default.
• W2113231874 cites W2125021618 @default.
• W2113231874 cites W2126619109 @default.
• W2113231874 cites W2127407003 @default.
• W2113231874 cites W2128944252 @default.
• W2113231874 cites W2142929893 @default.
• W2113231874 cites W2158483456 @default.
• W2113231874 cites W2158797055 @default.
• W2113231874 cites W2167441750 @default.
• W2113231874 cites W2171861123 @default.
• W2113231874 cites W3104096998 @default.
• W2113231874 cites W4244913051 @default.
• W2113231874 doi "https://doi.org/10.1073/pnas.0807514106" @default.
• W2113231874 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2634888" @default.
• W2113231874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19098105" @default.
• W2113231874 hasPublicationYear "2008" @default.
• W2113231874 type Work @default.
• W2113231874 sameAs 2113231874 @default.
• W2113231874 citedByCount "404" @default.
• W2113231874 countsByYear W21132318742012 @default.
• W2113231874 countsByYear W21132318742013 @default.
• W2113231874 countsByYear W21132318742014 @default.
• W2113231874 countsByYear W21132318742015 @default.
• W2113231874 countsByYear W21132318742016 @default.
• W2113231874 countsByYear W21132318742017 @default.
• W2113231874 countsByYear W21132318742018 @default.
• W2113231874 countsByYear W21132318742019 @default.
• W2113231874 countsByYear W21132318742020 @default.
• W2113231874 countsByYear W21132318742021 @default.
• W2113231874 countsByYear W21132318742022 @default.
• W2113231874 countsByYear W21132318742023 @default.
• W2113231874 crossrefType "journal-article" @default.
• W2113231874 hasAuthorship W2113231874A5018497969 @default.
• W2113231874 hasAuthorship W2113231874A5019834279 @default.
• W2113231874 hasAuthorship W2113231874A5036527909 @default.
• W2113231874 hasAuthorship W2113231874A5062657766 @default.
• W2113231874 hasAuthorship W2113231874A5085987918 @default.
• W2113231874 hasBestOaLocation W21132318742 @default.
• W2113231874 hasConcept C104317684 @default.
• W2113231874 hasConcept C12554922 @default.
• W2113231874 hasConcept C126513998 @default.
• W2113231874 hasConcept C141795571 @default.
• W2113231874 hasConcept C142724271 @default.
• W2113231874 hasConcept C143065580 @default.
• W2113231874 hasConcept C146587185 @default.
• W2113231874 hasConcept C171250308 @default.
• W2113231874 hasConcept C178790620 @default.
• W2113231874 hasConcept C185592680 @default.
• W2113231874 hasConcept C192562407 @default.
• W2113231874 hasConcept C2777204207 @default.
• W2113231874 hasConcept C2777317804 @default.
• W2113231874 hasConcept C2777975735 @default.
• W2113231874 hasConcept C2781069245 @default.
• W2113231874 hasConcept C32909587 @default.
• W2113231874 hasConcept C51679486 @default.
• W2113231874 hasConcept C547475151 @default.
• W2113231874 hasConcept C552990157 @default.
• W2113231874 hasConcept C55493867 @default.
• W2113231874 hasConcept C71924100 @default.
• W2113231874 hasConcept C86803240 @default.
• W2113231874 hasConceptScore W2113231874C104317684 @default.
• W2113231874 hasConceptScore W2113231874C12554922 @default.
• W2113231874 hasConceptScore W2113231874C126513998 @default.
• W2113231874 hasConceptScore W2113231874C141795571 @default.
• W2113231874 hasConceptScore W2113231874C142724271 @default.
• W2113231874 hasConceptScore W2113231874C143065580 @default.

• next