FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113265452> ?p ?o ?g. }

• W2113265452 endingPage "178" @default.
• W2113265452 startingPage "167" @default.
• W2113265452 abstract "A central issue in the application of probiotics as food additives is their fastidious production and their sensitivity to many environmental stresses. The importance of inducible cell-protective mechanisms triggered by application of sublethal stresses for survival under stress conditions has been demonstrated. Continuous cultures could be a suitable and more efficient method to test stress factors on one culture instead of several repeated batch cultures. In this study, the application of a two-stage continuous culture of Bifidobacterium longum NCC2705 was investigated. The first reactor was operated under fixed conditions at 37 °C and pH 6.0 and used to produce cells with controlled physiology, mimicking cells in the late exponential growth phase. Stress pretreatment combinations of pH (6.0, 5.0 and 4.0), temperature (37, 45 and 47 °C) and NaCl (0, 5 and 10%) were tested in the second reactor. Of all tested combinations, only those of pH 4.0 significantly decreased cell viability in the second reactor compared to control conditions (37 °C, pH 6.0, 0% NaCl) and, therefore, could not be considered as sublethal stresses. Pretreatments with 5 or 10% NaCl had a negative effect on cell viability after gastric lethal stress. A significant improvement in cell resistance to heat lethal stress (56 °C, 5 min) was observed for cells pretreated at 47 °C. In contrast, heat pretreatment negatively affected cell viability after freeze drying and osmotic lethal stresses. The two-stage continuous culture allowed for efficient screening of several stress pretreatments during the same experiment with up to four different conditions tested per day. Optimal sublethal stress conditions can also be applied for producing cells with traditional batch cultures." @default.
• W2113265452 created "2016-06-24" @default.
• W2113265452 creator A5007856392 @default.
• W2113265452 creator A5035906926 @default.
• W2113265452 creator A5044104887 @default.
• W2113265452 creator A5060356270 @default.
• W2113265452 creator A5072786950 @default.
• W2113265452 creator A5073823934 @default.
• W2113265452 date "2013-06-01" @default.
• W2113265452 modified "2023-09-27" @default.
• W2113265452 title "Fast screening of <i>Bifidobacterium longum</i> sublethal stress conditions in a novel two-stage continuous culture strategy" @default.
• W2113265452 cites W1820335481 @default.
• W2113265452 cites W2000018646 @default.
• W2113265452 cites W2011452694 @default.
• W2113265452 cites W2016260109 @default.
• W2113265452 cites W2022691623 @default.
• W2113265452 cites W2022990342 @default.
• W2113265452 cites W2025628074 @default.
• W2113265452 cites W2036088435 @default.
• W2113265452 cites W2044858769 @default.
• W2113265452 cites W2052028140 @default.
• W2113265452 cites W2055323481 @default.
• W2113265452 cites W2068904931 @default.
• W2113265452 cites W2076128170 @default.
• W2113265452 cites W2079966251 @default.
• W2113265452 cites W2084354778 @default.
• W2113265452 cites W2119999468 @default.
• W2113265452 cites W2158800024 @default.
• W2113265452 cites W4296587746 @default.
• W2113265452 doi "https://doi.org/10.3920/bm2012.0026" @default.
• W2113265452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23443949" @default.
• W2113265452 hasPublicationYear "2013" @default.
• W2113265452 type Work @default.
• W2113265452 sameAs 2113265452 @default.
• W2113265452 citedByCount "12" @default.
• W2113265452 countsByYear W21132654522013 @default.
• W2113265452 countsByYear W21132654522014 @default.
• W2113265452 countsByYear W21132654522015 @default.
• W2113265452 countsByYear W21132654522016 @default.
• W2113265452 countsByYear W21132654522019 @default.
• W2113265452 countsByYear W21132654522020 @default.
• W2113265452 countsByYear W21132654522021 @default.
• W2113265452 countsByYear W21132654522022 @default.
• W2113265452 crossrefType "journal-article" @default.
• W2113265452 hasAuthorship W2113265452A5007856392 @default.
• W2113265452 hasAuthorship W2113265452A5035906926 @default.
• W2113265452 hasAuthorship W2113265452A5044104887 @default.
• W2113265452 hasAuthorship W2113265452A5060356270 @default.
• W2113265452 hasAuthorship W2113265452A5072786950 @default.
• W2113265452 hasAuthorship W2113265452A5073823934 @default.
• W2113265452 hasConcept C100544194 @default.
• W2113265452 hasConcept C104317684 @default.
• W2113265452 hasConcept C1491633281 @default.
• W2113265452 hasConcept C16685009 @default.
• W2113265452 hasConcept C168529131 @default.
• W2113265452 hasConcept C185592680 @default.
• W2113265452 hasConcept C2776996202 @default.
• W2113265452 hasConcept C2780206646 @default.
• W2113265452 hasConcept C2780305828 @default.
• W2113265452 hasConcept C31903555 @default.
• W2113265452 hasConcept C43617362 @default.
• W2113265452 hasConcept C53227056 @default.
• W2113265452 hasConcept C54355233 @default.
• W2113265452 hasConcept C55493867 @default.
• W2113265452 hasConcept C71924100 @default.
• W2113265452 hasConcept C81885089 @default.
• W2113265452 hasConcept C86803240 @default.
• W2113265452 hasConcept C89423630 @default.
• W2113265452 hasConceptScore W2113265452C100544194 @default.
• W2113265452 hasConceptScore W2113265452C104317684 @default.
• W2113265452 hasConceptScore W2113265452C1491633281 @default.
• W2113265452 hasConceptScore W2113265452C16685009 @default.
• W2113265452 hasConceptScore W2113265452C168529131 @default.
• W2113265452 hasConceptScore W2113265452C185592680 @default.
• W2113265452 hasConceptScore W2113265452C2776996202 @default.
• W2113265452 hasConceptScore W2113265452C2780206646 @default.
• W2113265452 hasConceptScore W2113265452C2780305828 @default.
• W2113265452 hasConceptScore W2113265452C31903555 @default.
• W2113265452 hasConceptScore W2113265452C43617362 @default.
• W2113265452 hasConceptScore W2113265452C53227056 @default.
• W2113265452 hasConceptScore W2113265452C54355233 @default.
• W2113265452 hasConceptScore W2113265452C55493867 @default.
• W2113265452 hasConceptScore W2113265452C71924100 @default.
• W2113265452 hasConceptScore W2113265452C81885089 @default.
• W2113265452 hasConceptScore W2113265452C86803240 @default.
• W2113265452 hasConceptScore W2113265452C89423630 @default.
• W2113265452 hasIssue "2" @default.
• W2113265452 hasLocation W21132654521 @default.
• W2113265452 hasLocation W21132654522 @default.
• W2113265452 hasOpenAccess W2113265452 @default.
• W2113265452 hasPrimaryLocation W21132654521 @default.
• W2113265452 hasRelatedWork W2006260804 @default.
• W2113265452 hasRelatedWork W2074988338 @default.
• W2113265452 hasRelatedWork W2113265452 @default.
• W2113265452 hasRelatedWork W2115132266 @default.
• W2113265452 hasRelatedWork W2373629594 @default.
• W2113265452 hasRelatedWork W3158094404 @default.
• W2113265452 hasRelatedWork W3179583415 @default.

• next