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• W2113307910 abstract "BackgroundMost new tuberculosis vaccines will be administered as a booster to subjects primed with bacille Calmette-Guérin (BCG) during childhood MethodsWe investigated in vivo and in vitro immune responses to mycobacteria in human immunodeficiency virus (HIV)–positive subjects in Tanzania primed with BCG during childhood and entering a tuberculosis booster vaccine trial. Tests included intradermal skin testing for Mycobacterium tuberculosis purified protein derivative (PPD) and Mycobacterium avium sensitin (MAS); lymphocyte proliferation assays and interferon (IFN)–γ levels after stimulation with Mycobacterium vaccae sonicate (MVS), M. tuberculosis early secreted antigen (ESAT)–6, M. tuberculosis antigen 85 (Ag85), or M. tuberculosis whole-cell lysate (WCL); and determination of serum antibody to lipoarabinomannin (LAM) ResultsA total of 888 subjects with CD4 cell counts ⩾200 cells/mm3 were enrolled. PPD and MAS test results were positive in 34% and 30% of the subjects, respectively. Proliferative responses were detected as follows: MVS, 6%; Ag85, 24%; ESAT-6, 21%; and WCL, 59%. IFN-γ responses were 2%, 6%, 12%, and 38%, respectively. LAM antibody was detected in 28% of the subjects. Subjects were more likely to have detectable proliferative and IFN-γ responses if they had positive PPD test results or CD4 cell counts ⩾500 cells/mm3. Overall, 94% of the subjects had evidence of primed mycobacterial immune responses ConclusionOf HIV-positive BCG-immunized adults with CD4 cell counts ⩾200 cells/mm3 in Tanzania, 94% are primed for booster mycobacterial immunization" @default.
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• W2113307910 date "2007-01-01" @default.
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• W2113307910 title "Baseline Mycobacterial Immune Responses in HIV‐Infected Adults Primed with bacille Calmette‐Guérin during Childhood and Entering a Tuberculosis Booster Vaccine Trial" @default.
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• W2113307910 doi "https://doi.org/10.1086/509896" @default.
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