Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113316512> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2113316512 endingPage "840" @default.
- W2113316512 startingPage "837" @default.
- W2113316512 abstract "A simple and economical technique was developed to isolate and culture human arterial smooth muscle cells from chorionic plate vessels. Placentas from healthy women were collected at the time of term delivery. Chorionic plate arteries were identified, excised, and cut into small pieces. An explant technique was used to grow cultures of placental arterial smooth muscle (PASM) cells. Small pieces of vessel with lumens down were placed in 100-mm culture plates and grown in Dulbecco modified eagle medium and 10% fetal bovine serum. Cells appeared from explants within 1 week and grew to confluence in approximately 4 weeks. At confluence, PASM cell cultures had a uniform cell morphology that was characterized by elongated cells in parallel rows, typical of smooth muscle cells. Smooth muscle cell phenotype was evaluated by morphology and by immunoblotting and immunofluorescence of smooth muscle myofilament proteins. All PASM cell cultures expressed alpha-smooth muscle actin, beta-tropomyosin, and h-caldesmon. Expression was similar to that of human aortic smooth muscle cells, but not to endothelial cells or fibroblasts. PASM cells stained uniformly for alpha-smooth muscle actin and lacked staining for a fibroblast-specific antigen. PASM cells were evaluated for their response to inflammatory mediators, tumor necrosis factor-alpha, and interleukin-1beta by measurement of interleukin-8 production. Cells cultured for 18 hours showed a progressive increase in interleukin-8 production with time. Treatment with inflammatory mediators increased interleukin-8 production by 3-fold as compared with media control. This technique provides a simple method to obtain normal human arterial smooth muscle cells for in vitro studies of physiology and pathophysiology." @default.
- W2113316512 created "2016-06-24" @default.
- W2113316512 creator A5005794014 @default.
- W2113316512 creator A5045654579 @default.
- W2113316512 creator A5066765738 @default.
- W2113316512 creator A5071794846 @default.
- W2113316512 date "2004-04-01" @default.
- W2113316512 modified "2023-10-16" @default.
- W2113316512 title "Isolation and Culture of Arterial Smooth Muscle Cells From Human Placenta" @default.
- W2113316512 cites W1970677658 @default.
- W2113316512 cites W1987264256 @default.
- W2113316512 cites W2030604861 @default.
- W2113316512 cites W2120567214 @default.
- W2113316512 cites W2134610115 @default.
- W2113316512 cites W2157603347 @default.
- W2113316512 cites W2165581465 @default.
- W2113316512 cites W2274042922 @default.
- W2113316512 cites W2314944830 @default.
- W2113316512 doi "https://doi.org/10.1161/01.hyp.0000119191.33112.9c" @default.
- W2113316512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14967841" @default.
- W2113316512 hasPublicationYear "2004" @default.
- W2113316512 type Work @default.
- W2113316512 sameAs 2113316512 @default.
- W2113316512 citedByCount "50" @default.
- W2113316512 countsByYear W21133165122012 @default.
- W2113316512 countsByYear W21133165122013 @default.
- W2113316512 countsByYear W21133165122014 @default.
- W2113316512 countsByYear W21133165122015 @default.
- W2113316512 countsByYear W21133165122017 @default.
- W2113316512 countsByYear W21133165122018 @default.
- W2113316512 countsByYear W21133165122019 @default.
- W2113316512 countsByYear W21133165122021 @default.
- W2113316512 countsByYear W21133165122022 @default.
- W2113316512 countsByYear W21133165122023 @default.
- W2113316512 crossrefType "journal-article" @default.
- W2113316512 hasAuthorship W2113316512A5005794014 @default.
- W2113316512 hasAuthorship W2113316512A5045654579 @default.
- W2113316512 hasAuthorship W2113316512A5066765738 @default.
- W2113316512 hasAuthorship W2113316512A5071794846 @default.
- W2113316512 hasBestOaLocation W21133165121 @default.
- W2113316512 hasConcept C125705527 @default.
- W2113316512 hasConcept C134018914 @default.
- W2113316512 hasConcept C16685009 @default.
- W2113316512 hasConcept C207200792 @default.
- W2113316512 hasConcept C2779395532 @default.
- W2113316512 hasConcept C2779404806 @default.
- W2113316512 hasConcept C2992686903 @default.
- W2113316512 hasConcept C54355233 @default.
- W2113316512 hasConcept C71924100 @default.
- W2113316512 hasConcept C81885089 @default.
- W2113316512 hasConcept C86803240 @default.
- W2113316512 hasConcept C95444343 @default.
- W2113316512 hasConceptScore W2113316512C125705527 @default.
- W2113316512 hasConceptScore W2113316512C134018914 @default.
- W2113316512 hasConceptScore W2113316512C16685009 @default.
- W2113316512 hasConceptScore W2113316512C207200792 @default.
- W2113316512 hasConceptScore W2113316512C2779395532 @default.
- W2113316512 hasConceptScore W2113316512C2779404806 @default.
- W2113316512 hasConceptScore W2113316512C2992686903 @default.
- W2113316512 hasConceptScore W2113316512C54355233 @default.
- W2113316512 hasConceptScore W2113316512C71924100 @default.
- W2113316512 hasConceptScore W2113316512C81885089 @default.
- W2113316512 hasConceptScore W2113316512C86803240 @default.
- W2113316512 hasConceptScore W2113316512C95444343 @default.
- W2113316512 hasIssue "4" @default.
- W2113316512 hasLocation W21133165121 @default.
- W2113316512 hasLocation W21133165122 @default.
- W2113316512 hasLocation W21133165123 @default.
- W2113316512 hasOpenAccess W2113316512 @default.
- W2113316512 hasPrimaryLocation W21133165121 @default.
- W2113316512 hasRelatedWork W2005895082 @default.
- W2113316512 hasRelatedWork W2010763916 @default.
- W2113316512 hasRelatedWork W2033746536 @default.
- W2113316512 hasRelatedWork W2042625723 @default.
- W2113316512 hasRelatedWork W2049775377 @default.
- W2113316512 hasRelatedWork W2089829718 @default.
- W2113316512 hasRelatedWork W2101702094 @default.
- W2113316512 hasRelatedWork W2113723259 @default.
- W2113316512 hasRelatedWork W2357378207 @default.
- W2113316512 hasRelatedWork W2611214966 @default.
- W2113316512 hasVolume "43" @default.
- W2113316512 isParatext "false" @default.
- W2113316512 isRetracted "false" @default.
- W2113316512 magId "2113316512" @default.
- W2113316512 workType "article" @default.