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• W2113321565 endingPage "e61479" @default.
• W2113321565 startingPage "e61479" @default.
• W2113321565 abstract "Most genomic resources available for insects represent the Holometabola, which are insects that undergo complete metamorphosis like beetles and flies. In contrast, the Hemimetabola (direct developing insects), representing the basal branches of the insect tree, have very few genomic resources. We have therefore created a large and publicly available transcriptome for the hemimetabolous insect Gryllus bimaculatus (cricket), a well-developed laboratory model organism whose potential for functional genetic experiments is currently limited by the absence of genomic resources. cDNA was prepared using mRNA obtained from adult ovaries containing all stages of oogenesis, and from embryo samples on each day of embryogenesis. Using 454 Titanium pyrosequencing, we sequenced over four million raw reads, and assembled them into 21,512 isotigs (predicted transcripts) and 120,805 singletons with an average coverage per base pair of 51.3. We annotated the transcriptome manually for over 400 conserved genes involved in embryonic patterning, gametogenesis, and signaling pathways. BLAST comparison of the transcriptome against the NCBI non-redundant protein database (nr) identified significant similarity to nr sequences for 55.5% of transcriptome sequences, and suggested that the transcriptome may contain 19,874 unique transcripts. For predicted transcripts without significant similarity to known sequences, we assessed their similarity to other orthopteran sequences, and determined that these transcripts contain recognizable protein domains, largely of unknown function. We created a searchable, web-based database to allow public access to all raw, assembled and annotated data. This database is to our knowledge the largest de novo assembled and annotated transcriptome resource available for any hemimetabolous insect. We therefore anticipate that these data will contribute significantly to more effective and higher-throughput deployment of molecular analysis tools in Gryllus." @default.
• W2113321565 created "2016-06-24" @default.
• W2113321565 creator A5004023722 @default.
• W2113321565 creator A5016952505 @default.
• W2113321565 creator A5018215654 @default.
• W2113321565 creator A5020968428 @default.
• W2113321565 creator A5058519142 @default.
• W2113321565 creator A5081182542 @default.
• W2113321565 date "2013-05-06" @default.
• W2113321565 modified "2023-09-26" @default.
• W2113321565 title "Developmental Gene Discovery in a Hemimetabolous Insect: De Novo Assembly and Annotation of a Transcriptome for the Cricket Gryllus bimaculatus" @default.
• W2113321565 cites W1528131571 @default.
• W2113321565 cites W1590581394 @default.
• W2113321565 cites W1635391495 @default.
• W2113321565 cites W1768276073 @default.
• W2113321565 cites W1829422201 @default.
• W2113321565 cites W1846350123 @default.
• W2113321565 cites W1923089477 @default.
• W2113321565 cites W1965186869 @default.
• W2113321565 cites W1973811920 @default.
• W2113321565 cites W1975584335 @default.
• W2113321565 cites W1977830554 @default.
• W2113321565 cites W1985458708 @default.
• W2113321565 cites W1993679477 @default.
• W2113321565 cites W1996058782 @default.
• W2113321565 cites W1997696441 @default.
• W2113321565 cites W1997801717 @default.
• W2113321565 cites W1999098952 @default.
• W2113321565 cites W2002117801 @default.
• W2113321565 cites W2014719716 @default.
• W2113321565 cites W2015377991 @default.
• W2113321565 cites W2018543421 @default.
• W2113321565 cites W2018888972 @default.
• W2113321565 cites W2022366078 @default.
• W2113321565 cites W2029626836 @default.
• W2113321565 cites W2036791863 @default.
• W2113321565 cites W2040083508 @default.
• W2113321565 cites W2043865414 @default.
• W2113321565 cites W2044310691 @default.
• W2113321565 cites W2052688687 @default.
• W2113321565 cites W2053676247 @default.
• W2113321565 cites W2054378933 @default.
• W2113321565 cites W2056300001 @default.
• W2113321565 cites W2058816652 @default.
• W2113321565 cites W2062795065 @default.
• W2113321565 cites W2063512918 @default.
• W2113321565 cites W2064546612 @default.
• W2113321565 cites W2065684379 @default.
• W2113321565 cites W2076395090 @default.
• W2113321565 cites W2077697996 @default.
• W2113321565 cites W2080759256 @default.
• W2113321565 cites W2083147249 @default.
• W2113321565 cites W2084118665 @default.
• W2113321565 cites W2091448229 @default.
• W2113321565 cites W2093327506 @default.
• W2113321565 cites W2096008949 @default.
• W2113321565 cites W2096845591 @default.
• W2113321565 cites W2097073373 @default.
• W2113321565 cites W2099946731 @default.
• W2113321565 cites W2100703845 @default.
• W2113321565 cites W2102638056 @default.
• W2113321565 cites W2103288430 @default.
• W2113321565 cites W2108977919 @default.
• W2113321565 cites W2111373249 @default.
• W2113321565 cites W2111946775 @default.
• W2113321565 cites W2112250822 @default.
• W2113321565 cites W2114899166 @default.
• W2113321565 cites W2116363471 @default.
• W2113321565 cites W2117936709 @default.
• W2113321565 cites W2118059471 @default.
• W2113321565 cites W2119923823 @default.
• W2113321565 cites W2121016876 @default.
• W2113321565 cites W2125365332 @default.
• W2113321565 cites W2126975650 @default.
• W2113321565 cites W2128174440 @default.
• W2113321565 cites W2129710424 @default.
• W2113321565 cites W2133818514 @default.
• W2113321565 cites W2136774840 @default.
• W2113321565 cites W2141652419 @default.
• W2113321565 cites W2143335572 @default.
• W2113321565 cites W2152073175 @default.
• W2113321565 cites W2153569313 @default.
• W2113321565 cites W2155249372 @default.
• W2113321565 cites W2158310129 @default.
• W2113321565 cites W2158605937 @default.
• W2113321565 cites W2160522042 @default.
• W2113321565 cites W2161241566 @default.
• W2113321565 cites W2161746138 @default.
• W2113321565 cites W2163105490 @default.
• W2113321565 cites W2163181687 @default.
• W2113321565 cites W2167197805 @default.
• W2113321565 cites W2168223461 @default.
• W2113321565 cites W2171311206 @default.
• W2113321565 cites W2171437346 @default.
• W2113321565 cites W2180831823 @default.
• W2113321565 cites W2486234036 @default.
• W2113321565 cites W2505296973 @default.
• W2113321565 cites W4210400672 @default.

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