FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113343524> ?p ?o ?g. }

• W2113343524 abstract "Background: Neisseria meningitidis Serogroup A, is a major cause of bacterial meningitidis outbreaks in Africa and the Middle East. While polysaccharide vaccines have been available for many years, these vaccines have many disadvantages including the induction of T-cell independent responses which do not induce memory responses. Objectives: Thus to overcome this problem, in this research outer membrane vesicle (OMV) containing PorA was extracted and evaluated by biological and immunological methods. Materials and Methods: OMVs were extracted with deoxycholate and EDTA, and purification was performed by sequential ultracentrifugation. Physicochemical properties of extracted OMVs were analyzed by electron microscopy and SDS-PAGE. The toxicity of LPS content in its was assayed by LAL test. The Presence of PorA as a major component of OMV was confirmed by western blot. To study antibodies synthesis after immunization with OMV, ELISA method was used. Also serum bactericidal assay (SBA) was performed to determine the serum bactericidal activity against N. meningitidis serogroup A. Results: The results revealed that the content of protein extracted was 0.1 mg/mL. The electron microscopy showed that intactness of the vesicle in these preparation ranged more than 70%. The SDS-PAGE showed that PorA as a major immunological part of outer membrane vesicle was located in 35-40kDa. LAL test showed that the endotoxin activity was around 126EU/mL which is safe for using. The ELISA test revealed that the IgG total titer was elevated after the first injection. SBA indicates that bactericidal antibodies rise after the second dose of booster. Conclusions: The results showed that the extracted OMVs were conformationally stable, and there were no pyrogenic determinants in OMV. Also the results showed that the OMV elicited high level of specific antibodies against N. meningitidis serogroup A. These results indicate that the OMV obtained here, can be used as a meningococcal vaccine after further investigation." @default.
• W2113343524 created "2016-06-24" @default.
• W2113343524 creator A5003202834 @default.
• W2113343524 creator A5007310288 @default.
• W2113343524 creator A5014092450 @default.
• W2113343524 creator A5022402107 @default.
• W2113343524 creator A5038927647 @default.
• W2113343524 creator A5050345200 @default.
• W2113343524 creator A5057491166 @default.
• W2113343524 creator A5060321921 @default.
• W2113343524 creator A5069837203 @default.
• W2113343524 creator A5072374394 @default.
• W2113343524 creator A5075102846 @default.
• W2113343524 creator A5084050163 @default.
• W2113343524 date "2013-07-01" @default.
• W2113343524 modified "2023-09-26" @default.
• W2113343524 title "Biological and Immunological Evaluation of <italic>Neisseria meningitidis</italic> Serogroup A Outer Membrane Vesicle as Vaccine Candidates" @default.
• W2113343524 cites W1509321259 @default.
• W2113343524 cites W1996141674 @default.
• W2113343524 cites W2040481026 @default.
• W2113343524 cites W2040626353 @default.
• W2113343524 cites W2044087450 @default.
• W2113343524 cites W2051513982 @default.
• W2113343524 cites W2066398297 @default.
• W2113343524 cites W2104963250 @default.
• W2113343524 cites W2129054495 @default.
• W2113343524 cites W2146415650 @default.
• W2113343524 cites W2150102406 @default.
• W2113343524 cites W2159656449 @default.
• W2113343524 cites W2165839096 @default.
• W2113343524 cites W1995191396 @default.
• W2113343524 doi "https://doi.org/10.5812/jjm.5007" @default.
• W2113343524 hasPublicationYear "2013" @default.
• W2113343524 type Work @default.
• W2113343524 sameAs 2113343524 @default.
• W2113343524 citedByCount "4" @default.
• W2113343524 countsByYear W21133435242018 @default.
• W2113343524 countsByYear W21133435242020 @default.
• W2113343524 crossrefType "journal-article" @default.
• W2113343524 hasAuthorship W2113343524A5003202834 @default.
• W2113343524 hasAuthorship W2113343524A5007310288 @default.
• W2113343524 hasAuthorship W2113343524A5014092450 @default.
• W2113343524 hasAuthorship W2113343524A5022402107 @default.
• W2113343524 hasAuthorship W2113343524A5038927647 @default.
• W2113343524 hasAuthorship W2113343524A5050345200 @default.
• W2113343524 hasAuthorship W2113343524A5057491166 @default.
• W2113343524 hasAuthorship W2113343524A5060321921 @default.
• W2113343524 hasAuthorship W2113343524A5069837203 @default.
• W2113343524 hasAuthorship W2113343524A5072374394 @default.
• W2113343524 hasAuthorship W2113343524A5075102846 @default.
• W2113343524 hasAuthorship W2113343524A5084050163 @default.
• W2113343524 hasBestOaLocation W21133435241 @default.
• W2113343524 hasConcept C104317684 @default.
• W2113343524 hasConcept C130316041 @default.
• W2113343524 hasConcept C146587185 @default.
• W2113343524 hasConcept C159047783 @default.
• W2113343524 hasConcept C159654299 @default.
• W2113343524 hasConcept C185592680 @default.
• W2113343524 hasConcept C203014093 @default.
• W2113343524 hasConcept C2776415932 @default.
• W2113343524 hasConcept C2778458888 @default.
• W2113343524 hasConcept C32611913 @default.
• W2113343524 hasConcept C41625074 @default.
• W2113343524 hasConcept C523546767 @default.
• W2113343524 hasConcept C54355233 @default.
• W2113343524 hasConcept C547475151 @default.
• W2113343524 hasConcept C55493867 @default.
• W2113343524 hasConcept C86803240 @default.
• W2113343524 hasConcept C89423630 @default.
• W2113343524 hasConceptScore W2113343524C104317684 @default.
• W2113343524 hasConceptScore W2113343524C130316041 @default.
• W2113343524 hasConceptScore W2113343524C146587185 @default.
• W2113343524 hasConceptScore W2113343524C159047783 @default.
• W2113343524 hasConceptScore W2113343524C159654299 @default.
• W2113343524 hasConceptScore W2113343524C185592680 @default.
• W2113343524 hasConceptScore W2113343524C203014093 @default.
• W2113343524 hasConceptScore W2113343524C2776415932 @default.
• W2113343524 hasConceptScore W2113343524C2778458888 @default.
• W2113343524 hasConceptScore W2113343524C32611913 @default.
• W2113343524 hasConceptScore W2113343524C41625074 @default.
• W2113343524 hasConceptScore W2113343524C523546767 @default.
• W2113343524 hasConceptScore W2113343524C54355233 @default.
• W2113343524 hasConceptScore W2113343524C547475151 @default.
• W2113343524 hasConceptScore W2113343524C55493867 @default.
• W2113343524 hasConceptScore W2113343524C86803240 @default.
• W2113343524 hasConceptScore W2113343524C89423630 @default.
• W2113343524 hasIssue "4" @default.
• W2113343524 hasLocation W21133435241 @default.
• W2113343524 hasOpenAccess W2113343524 @default.
• W2113343524 hasPrimaryLocation W21133435241 @default.
• W2113343524 hasRelatedWork W1491850971 @default.
• W2113343524 hasRelatedWork W1984647379 @default.
• W2113343524 hasRelatedWork W1987735630 @default.
• W2113343524 hasRelatedWork W2042977685 @default.
• W2113343524 hasRelatedWork W2053749166 @default.
• W2113343524 hasRelatedWork W2102044733 @default.
• W2113343524 hasRelatedWork W2108897235 @default.
• W2113343524 hasRelatedWork W2415986942 @default.
• W2113343524 hasRelatedWork W2784353907 @default.
• W2113343524 hasRelatedWork W2958384199 @default.

• next