FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113361833> ?p ?o ?g. }

• W2113361833 endingPage "1856" @default.
• W2113361833 startingPage "1856" @default.
• W2113361833 abstract "A hemodynamic Lab-on-a-chip system was developed in this study. This system has two unique features: (1) it consists of a microfluidic network with an array of endothelial cell seeding sites for testing them under multiple conditions, and (2) the flow rate and the frequency of the culture medium in the microchannel are controlled by a pulsation free pump to mimic the flow profile of the blood in the blood vessel under different physiological conditions. The investigated physiological conditions were: (1) the resting condition in a normal shear stress of 15 dyne cm−2 with a normal heart rate of 70 bpm, (2) an exhaustive exercise condition with a high shear stress of 30 dyne cm−2 and a fast heart rate of 140 bpm, and (3) a constant high shear stress of 30 dyne cm−2. Two chemical conditions were investigated (10 mM and 20 mM glucose) to mimic hyperglycemic conditions in diabetes patients. The effects of various shear stresses either alone or in combination with different glucose concentrations on endothelial cells were examined using the developed hemodynamic Lab-on-a-chip system by assessing two parameters. One is the intracellular level of reactive oxygen species (ROS) determined by a fluorescent probe, H2DCFDA. Another is the mitochondrial morphology revealed with a fluorescent dye, MitoTracker Green FM. The results showed that ROS level was elevated nearly 4-fold after 60 min of exhaustive exercise. We found that the pulsatile nature of the fluid was the determination factor for causing ROS generation in the cells as almost no increase of ROS was detected in the constant shear stress condition. Similarly, much higher level of ROS was detected when 10 mM glucose was applied to the cells under normal or high pulsatile shear stresses compared with under a static condition. These results suggest that it is necessary to use pulsatile shear stress to represent the physiological conditions of the blood flow, and demonstrate the advantage of utilizing this newly developed hemodynamic Lab-on-a-chip system over the conventional non-pulsatile system in the future shear stress related studies." @default.
• W2113361833 created "2016-06-24" @default.
• W2113361833 creator A5016348619 @default.
• W2113361833 creator A5016947185 @default.
• W2113361833 creator A5047931885 @default.
• W2113361833 creator A5058709537 @default.
• W2113361833 creator A5081288356 @default.
• W2113361833 creator A5087161143 @default.
• W2113361833 date "2011-01-01" @default.
• W2113361833 modified "2023-10-18" @default.
• W2113361833 title "Production of reactive oxygen species in endothelial cells under different pulsatile shear stresses and glucose concentrations" @default.
• W2113361833 cites W143835407 @default.
• W2113361833 cites W1516429022 @default.
• W2113361833 cites W1591456752 @default.
• W2113361833 cites W1936770188 @default.
• W2113361833 cites W1970024800 @default.
• W2113361833 cites W1973407863 @default.
• W2113361833 cites W1986568749 @default.
• W2113361833 cites W2002499212 @default.
• W2113361833 cites W2006385054 @default.
• W2113361833 cites W2011988006 @default.
• W2113361833 cites W2012487082 @default.
• W2113361833 cites W2021457420 @default.
• W2113361833 cites W2030817463 @default.
• W2113361833 cites W2033117797 @default.
• W2113361833 cites W2033800732 @default.
• W2113361833 cites W2035894729 @default.
• W2113361833 cites W2037305458 @default.
• W2113361833 cites W2076852139 @default.
• W2113361833 cites W2101861123 @default.
• W2113361833 cites W2109168147 @default.
• W2113361833 cites W2113472065 @default.
• W2113361833 cites W2131666074 @default.
• W2113361833 cites W2134256354 @default.
• W2113361833 cites W2134688511 @default.
• W2113361833 cites W2139130558 @default.
• W2113361833 cites W2221286231 @default.
• W2113361833 doi "https://doi.org/10.1039/c0lc00651c" @default.
• W2113361833 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21373653" @default.
• W2113361833 hasPublicationYear "2011" @default.
• W2113361833 type Work @default.
• W2113361833 sameAs 2113361833 @default.
• W2113361833 citedByCount "78" @default.
• W2113361833 countsByYear W21133618332012 @default.
• W2113361833 countsByYear W21133618332013 @default.
• W2113361833 countsByYear W21133618332014 @default.
• W2113361833 countsByYear W21133618332015 @default.
• W2113361833 countsByYear W21133618332016 @default.
• W2113361833 countsByYear W21133618332017 @default.
• W2113361833 countsByYear W21133618332018 @default.
• W2113361833 countsByYear W21133618332019 @default.
• W2113361833 countsByYear W21133618332020 @default.
• W2113361833 countsByYear W21133618332021 @default.
• W2113361833 countsByYear W21133618332022 @default.
• W2113361833 countsByYear W21133618332023 @default.
• W2113361833 crossrefType "journal-article" @default.
• W2113361833 hasAuthorship W2113361833A5016348619 @default.
• W2113361833 hasAuthorship W2113361833A5016947185 @default.
• W2113361833 hasAuthorship W2113361833A5047931885 @default.
• W2113361833 hasAuthorship W2113361833A5058709537 @default.
• W2113361833 hasAuthorship W2113361833A5081288356 @default.
• W2113361833 hasAuthorship W2113361833A5087161143 @default.
• W2113361833 hasConcept C12554922 @default.
• W2113361833 hasConcept C126322002 @default.
• W2113361833 hasConcept C136229726 @default.
• W2113361833 hasConcept C159985019 @default.
• W2113361833 hasConcept C165682214 @default.
• W2113361833 hasConcept C171250308 @default.
• W2113361833 hasConcept C178853913 @default.
• W2113361833 hasConcept C185592680 @default.
• W2113361833 hasConcept C192562407 @default.
• W2113361833 hasConcept C200990466 @default.
• W2113361833 hasConcept C21141959 @default.
• W2113361833 hasConcept C48349386 @default.
• W2113361833 hasConcept C55493867 @default.
• W2113361833 hasConcept C63662833 @default.
• W2113361833 hasConcept C71924100 @default.
• W2113361833 hasConcept C79191276 @default.
• W2113361833 hasConcept C8673954 @default.
• W2113361833 hasConcept C86803240 @default.
• W2113361833 hasConceptScore W2113361833C12554922 @default.
• W2113361833 hasConceptScore W2113361833C126322002 @default.
• W2113361833 hasConceptScore W2113361833C136229726 @default.
• W2113361833 hasConceptScore W2113361833C159985019 @default.
• W2113361833 hasConceptScore W2113361833C165682214 @default.
• W2113361833 hasConceptScore W2113361833C171250308 @default.
• W2113361833 hasConceptScore W2113361833C178853913 @default.
• W2113361833 hasConceptScore W2113361833C185592680 @default.
• W2113361833 hasConceptScore W2113361833C192562407 @default.
• W2113361833 hasConceptScore W2113361833C200990466 @default.
• W2113361833 hasConceptScore W2113361833C21141959 @default.
• W2113361833 hasConceptScore W2113361833C48349386 @default.
• W2113361833 hasConceptScore W2113361833C55493867 @default.
• W2113361833 hasConceptScore W2113361833C63662833 @default.
• W2113361833 hasConceptScore W2113361833C71924100 @default.
• W2113361833 hasConceptScore W2113361833C79191276 @default.
• W2113361833 hasConceptScore W2113361833C8673954 @default.
• W2113361833 hasConceptScore W2113361833C86803240 @default.

• next