FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113405529> ?p ?o ?g. }

• W2113405529 endingPage "9414" @default.
• W2113405529 startingPage "9407" @default.
• W2113405529 abstract "ABSTRACT A duplication of the polypurine tract (PPT) at the center of the human immunodeficiency virus type 1 (HIV-1) genome (the cPPT) has been shown to prime a separate plus-strand initiation and to result in a plus-strand displacement (DNA flap) that plays a role in nuclear import of the viral preintegration complex. Feline immunodeficiency virus (FIV) is a lentivirus that infects nondividing cells, causes progressive CD4 + T-cell depletion, and has been used as a substrate for lentiviral vectors. However, the PPT sequence is not duplicated elsewhere in the FIV genome and a central plus-strand initiation or strand displacement has not been identified. Using Southern blotting of S1 nuclease-digested FIV preintegration complexes isolated from infected cells, we detected a single-strand discontinuity at the approximate center of the reverse-transcribed genome. Primer extension analyses assigned the gap to the plus strand, and mapped the 5′ terminus of the downstream (D+) segment to a guanine residue in a purine-rich tract in pol (AAAAGAAGAGGTA G GA). RACE experiments then mapped the 3′ terminus of the upstream plus (U+)-strand segment to a T nucleotide located 88 nucleotides downstream of the D+ strand 5′ terminus, thereby identifying the extent of D+ strand displacement and the central termination sequence of this virus. Unlike HIV, the FIV cPPT is significantly divergent in sequence from its 3′ counterpart (AAAAAAGAAAAAAGGGTGG) and contains one and in some cases two pyrimidines. An invariant thymidine located −2 to the D+ strand origin is neither required nor optimal for codon usage at this position. Although the mapped cPPTs of FIV and HIV-1 act in cis , they encode homologous amino acids in integrase." @default.
• W2113405529 created "2016-06-24" @default.
• W2113405529 creator A5013651117 @default.
• W2113405529 creator A5022571156 @default.
• W2113405529 creator A5073936119 @default.
• W2113405529 date "2001-10-01" @default.
• W2113405529 modified "2023-09-27" @default.
• W2113405529 title "Identification of a Central DNA Flap in Feline Immunodeficiency Virus" @default.
• W2113405529 cites W1488580265 @default.
• W2113405529 cites W1525987134 @default.
• W2113405529 cites W1555146480 @default.
• W2113405529 cites W1564941168 @default.
• W2113405529 cites W1566969252 @default.
• W2113405529 cites W1579646504 @default.
• W2113405529 cites W1587940136 @default.
• W2113405529 cites W1738945493 @default.
• W2113405529 cites W1918197787 @default.
• W2113405529 cites W1967446284 @default.
• W2113405529 cites W1981585109 @default.
• W2113405529 cites W2012290180 @default.
• W2113405529 cites W2014970708 @default.
• W2113405529 cites W2030017358 @default.
• W2113405529 cites W2030220702 @default.
• W2113405529 cites W2037835396 @default.
• W2113405529 cites W2047028501 @default.
• W2113405529 cites W2057554454 @default.
• W2113405529 cites W2057814775 @default.
• W2113405529 cites W2066753492 @default.
• W2113405529 cites W2070960340 @default.
• W2113405529 cites W2079939178 @default.
• W2113405529 cites W2080589169 @default.
• W2113405529 cites W2087809591 @default.
• W2113405529 cites W2089590026 @default.
• W2113405529 cites W2096996442 @default.
• W2113405529 cites W2100535431 @default.
• W2113405529 cites W2101765956 @default.
• W2113405529 cites W2103231142 @default.
• W2113405529 cites W2113786483 @default.
• W2113405529 cites W2117149353 @default.
• W2113405529 cites W2127238148 @default.
• W2113405529 cites W2128843867 @default.
• W2113405529 cites W2142753618 @default.
• W2113405529 cites W2156527368 @default.
• W2113405529 cites W2502788995 @default.
• W2113405529 doi "https://doi.org/10.1128/jvi.75.19.9407-9414.2001" @default.
• W2113405529 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/114508" @default.
• W2113405529 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11533203" @default.
• W2113405529 hasPublicationYear "2001" @default.
• W2113405529 type Work @default.
• W2113405529 sameAs 2113405529 @default.
• W2113405529 citedByCount "45" @default.
• W2113405529 countsByYear W21134055292012 @default.
• W2113405529 countsByYear W21134055292013 @default.
• W2113405529 countsByYear W21134055292014 @default.
• W2113405529 countsByYear W21134055292015 @default.
• W2113405529 countsByYear W21134055292017 @default.
• W2113405529 countsByYear W21134055292023 @default.
• W2113405529 crossrefType "journal-article" @default.
• W2113405529 hasAuthorship W2113405529A5013651117 @default.
• W2113405529 hasAuthorship W2113405529A5022571156 @default.
• W2113405529 hasAuthorship W2113405529A5073936119 @default.
• W2113405529 hasBestOaLocation W21134055292 @default.
• W2113405529 hasConcept C153911025 @default.
• W2113405529 hasConcept C159047783 @default.
• W2113405529 hasConcept C178790620 @default.
• W2113405529 hasConcept C185592680 @default.
• W2113405529 hasConcept C2522874641 @default.
• W2113405529 hasConcept C2777271071 @default.
• W2113405529 hasConcept C2777563447 @default.
• W2113405529 hasConcept C2777841672 @default.
• W2113405529 hasConcept C2779690655 @default.
• W2113405529 hasConcept C2780727368 @default.
• W2113405529 hasConcept C54355233 @default.
• W2113405529 hasConcept C552990157 @default.
• W2113405529 hasConcept C86803240 @default.
• W2113405529 hasConceptScore W2113405529C153911025 @default.
• W2113405529 hasConceptScore W2113405529C159047783 @default.
• W2113405529 hasConceptScore W2113405529C178790620 @default.
• W2113405529 hasConceptScore W2113405529C185592680 @default.
• W2113405529 hasConceptScore W2113405529C2522874641 @default.
• W2113405529 hasConceptScore W2113405529C2777271071 @default.
• W2113405529 hasConceptScore W2113405529C2777563447 @default.
• W2113405529 hasConceptScore W2113405529C2777841672 @default.
• W2113405529 hasConceptScore W2113405529C2779690655 @default.
• W2113405529 hasConceptScore W2113405529C2780727368 @default.
• W2113405529 hasConceptScore W2113405529C54355233 @default.
• W2113405529 hasConceptScore W2113405529C552990157 @default.
• W2113405529 hasConceptScore W2113405529C86803240 @default.
• W2113405529 hasIssue "19" @default.
• W2113405529 hasLocation W21134055291 @default.
• W2113405529 hasLocation W21134055292 @default.
• W2113405529 hasLocation W21134055293 @default.
• W2113405529 hasLocation W21134055294 @default.
• W2113405529 hasOpenAccess W2113405529 @default.
• W2113405529 hasPrimaryLocation W21134055291 @default.
• W2113405529 hasRelatedWork W1563639362 @default.
• W2113405529 hasRelatedWork W1951049417 @default.
• W2113405529 hasRelatedWork W1964842398 @default.

• next