Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113443543> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2113443543 endingPage "e86" @default.
- W2113443543 startingPage "e86" @default.
- W2113443543 abstract "Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses T4 RNA ligase to attach a 3′-biotinylated donor molecule to target RNA. Microarray analyses indicate that directly labeled RNA is uniformly labeled, has higher signal intensity than comparable labeling methods and achieves high transcript detection sensitivity. The labeled donor molecule we have developed allows the attachment of multiple biotins, which increases target signal intensity up to 30%. We have used this direct-labeling method to detect previously discovered class predictor genes for two types of cancer: acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). In order to test the sensitivity of direct RNA labeling, we analyzed the AML and ALL expression profiles for predictor genes that were previously found to show elevated expression in the disease state. Direct labeling of AML poly(A) RNA detects 90% of the class predictor genes that are detected by the IVT-based target amplification method used to discover the genes. These results indicate that the detection sensitivity, simplicity (single tube reaction) and speed (2 h) of this direct labeling protocol may be ideal for diagnostic applications that do not require target amplification." @default.
- W2113443543 created "2016-06-24" @default.
- W2113443543 creator A5005796905 @default.
- W2113443543 date "2004-06-21" @default.
- W2113443543 modified "2023-10-03" @default.
- W2113443543 title "Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes" @default.
- W2113443543 cites W1496392745 @default.
- W2113443543 cites W1521380130 @default.
- W2113443543 cites W1582006209 @default.
- W2113443543 cites W1989697812 @default.
- W2113443543 cites W1991524704 @default.
- W2113443543 cites W2004601877 @default.
- W2113443543 cites W2010967238 @default.
- W2113443543 cites W2042404042 @default.
- W2113443543 cites W2042914662 @default.
- W2113443543 cites W2062036406 @default.
- W2113443543 cites W2064290022 @default.
- W2113443543 cites W2065170566 @default.
- W2113443543 cites W2072713437 @default.
- W2113443543 cites W2079575026 @default.
- W2113443543 cites W2086842226 @default.
- W2113443543 cites W2109363337 @default.
- W2113443543 cites W2109938396 @default.
- W2113443543 cites W2119560696 @default.
- W2113443543 cites W2123248953 @default.
- W2113443543 cites W2137510887 @default.
- W2113443543 cites W2143593381 @default.
- W2113443543 cites W2146007051 @default.
- W2113443543 cites W2339359457 @default.
- W2113443543 cites W2402766024 @default.
- W2113443543 cites W4246697467 @default.
- W2113443543 cites W4247420482 @default.
- W2113443543 doi "https://doi.org/10.1093/nar/gnh085" @default.
- W2113443543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/443553" @default.
- W2113443543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15205470" @default.
- W2113443543 hasPublicationYear "2004" @default.
- W2113443543 type Work @default.
- W2113443543 sameAs 2113443543 @default.
- W2113443543 citedByCount "32" @default.
- W2113443543 countsByYear W21134435432012 @default.
- W2113443543 countsByYear W21134435432013 @default.
- W2113443543 countsByYear W21134435432015 @default.
- W2113443543 countsByYear W21134435432017 @default.
- W2113443543 countsByYear W21134435432019 @default.
- W2113443543 countsByYear W21134435432020 @default.
- W2113443543 countsByYear W21134435432021 @default.
- W2113443543 countsByYear W21134435432023 @default.
- W2113443543 crossrefType "journal-article" @default.
- W2113443543 hasAuthorship W2113443543A5005796905 @default.
- W2113443543 hasBestOaLocation W21134435431 @default.
- W2113443543 hasConcept C104317684 @default.
- W2113443543 hasConcept C150194340 @default.
- W2113443543 hasConcept C153911025 @default.
- W2113443543 hasConcept C18431079 @default.
- W2113443543 hasConcept C186836561 @default.
- W2113443543 hasConcept C204628709 @default.
- W2113443543 hasConcept C2778729363 @default.
- W2113443543 hasConcept C502942594 @default.
- W2113443543 hasConcept C54355233 @default.
- W2113443543 hasConcept C67705224 @default.
- W2113443543 hasConcept C86803240 @default.
- W2113443543 hasConcept C95371953 @default.
- W2113443543 hasConceptScore W2113443543C104317684 @default.
- W2113443543 hasConceptScore W2113443543C150194340 @default.
- W2113443543 hasConceptScore W2113443543C153911025 @default.
- W2113443543 hasConceptScore W2113443543C18431079 @default.
- W2113443543 hasConceptScore W2113443543C186836561 @default.
- W2113443543 hasConceptScore W2113443543C204628709 @default.
- W2113443543 hasConceptScore W2113443543C2778729363 @default.
- W2113443543 hasConceptScore W2113443543C502942594 @default.
- W2113443543 hasConceptScore W2113443543C54355233 @default.
- W2113443543 hasConceptScore W2113443543C67705224 @default.
- W2113443543 hasConceptScore W2113443543C86803240 @default.
- W2113443543 hasConceptScore W2113443543C95371953 @default.
- W2113443543 hasIssue "11" @default.
- W2113443543 hasLocation W21134435431 @default.
- W2113443543 hasLocation W21134435432 @default.
- W2113443543 hasLocation W21134435433 @default.
- W2113443543 hasLocation W21134435434 @default.
- W2113443543 hasOpenAccess W2113443543 @default.
- W2113443543 hasPrimaryLocation W21134435431 @default.
- W2113443543 hasRelatedWork W1971076179 @default.
- W2113443543 hasRelatedWork W1996185163 @default.
- W2113443543 hasRelatedWork W2021297725 @default.
- W2113443543 hasRelatedWork W2053053668 @default.
- W2113443543 hasRelatedWork W2118890427 @default.
- W2113443543 hasRelatedWork W2362947020 @default.
- W2113443543 hasRelatedWork W2385534162 @default.
- W2113443543 hasRelatedWork W2773571775 @default.
- W2113443543 hasRelatedWork W3031950498 @default.
- W2113443543 hasRelatedWork W3032874291 @default.
- W2113443543 hasVolume "32" @default.
- W2113443543 isParatext "false" @default.
- W2113443543 isRetracted "false" @default.
- W2113443543 magId "2113443543" @default.
- W2113443543 workType "article" @default.