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- W2113444993 abstract "The cybB gene on a plasmid encoding cytochrome b561 in Escherichia coli was disrupted by insertion of Kmrl determinant DNA. The cromosomal cybB gene was replaced by the inactivated cybB gene on the plasmid by homologous recombination using λ phage lysogenization and heat-induction. The replacement was confirmed by Southern and Western blotting analyses. Deficiency on the cybB gene product did not affect the growth properties of the cells, and the oxidase activities of the cells dependent on various substrates were similar to those of the parental strain. Cytochrome b561 is concluded to be expressed in E. coli, but may not play a major role in cell growth. In the genetic map of E. coli, the cybB gene was determined by conjugational and transductional crosses to be at 31 min between trg and terC." @default.
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- W2113444993 date "1988-11-01" @default.
- W2113444993 modified "2023-09-27" @default.
- W2113444993 title "Mapping and disruption of the cybB gene coding for cytochrome b561 in Escherichia coli" @default.
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- W2113444993 doi "https://doi.org/10.1111/j.1574-6968.1988.tb03144.x" @default.
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