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• W2113480230 startingPage "1841" @default.
• W2113480230 abstract "When mutant strain UV77–247 of Prototheca moriformis Kruger was fed d‐[1‐13C]Glc, it synthesized l‐ascorbic acid (AA) with approximately three‐quarters of the label at the C‐1 position and the remaining label at the C‐6 position, showing that AA is made by a non‐inversion (retention) pathway, i.e. C‐1 of Glc becomes C‐1 of AA. The label present at C‐6 is consistent with the glycolytic conversion of Glc to 3‐carbon intermediates and subsequent gluconeogenesis. Compounds suggested as intermediates in inversion‐type pathways were not converted to AA. Most strains converted Man to AA at a rate greater than they did Glc. Enzyme activities leading from Fru‐6‐P to the formation of GDP‐Man were identified in all strains, but none of these activities correlated with the mutants’ abilities to accumulate AA. However, there was a strong correlation between GDP‐Man‐3,5‐epimerase activity and AA accumulation. Wild‐type P. moriformis ATCC 75669 and mutant strains of varying AA‐synthesizing abilities rapidly converted l‐Gal or l‐galactono‐1,4‐lactone to AA. Based on this data, a biosynthetic pathway from Glc to AA is proposed in which the epimerase is the rate‐limiting activity in AA synthesis." @default.
• W2113480230 created "2016-06-24" @default.
• W2113480230 creator A5061546843 @default.
• W2113480230 date "2003-08-01" @default.
• W2113480230 modified "2023-09-27" @default.
• W2113480230 title "The pathway of L-ascorbic acid biosynthesis in the colourless microalga Prototheca moriformis" @default.
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• W2113480230 doi "https://doi.org/10.1093/jxb/erg207" @default.
• W2113480230 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12869519" @default.
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