Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113488501> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2113488501 endingPage "916" @default.
- W2113488501 startingPage "911" @default.
- W2113488501 abstract "돼지 Duroc 품종의 mitochondria DNA D-loop전체 유전자를 증폭하기 위하여 많은 동물에서 고도로 상동성이 높은 tRNA-Pro와 tRNA-Phe 염기서열 일부를 이용하여 oligonucleotide primer를 제작하였다. 그 결과 Duroc 품종의 D-loop 전체 유전자는 1,145 base pairs 였으며, 그 중간위치에 10bp의 Sus Scrofa-specific sequence (TACACGTGCG)가 10개 존재하고 있었다. 돌연변이 검출을 위하여 가장 변이가 심한 지역을 primer 제작하여 345 bp의 DNA 단편을 증폭하였으며, Single Stranded Conformation Polymorphism(SSCP) 분석은 8% polyacrylamide gel에서 200 V, 16시간 전기영동하여 ethidium bromide (EtBr)로 10분간 염색하여 UV image analyzer로 관찰하였다. 그 결과 두 개의 서로 다른 밴드유형을 관찰하였으며, 21개 부위에서 염기서열 변이가 관찰되었다. 이러한 결과는 유전적 다양성 변이를 검출하는데 SSCP 분석이 유용한 도구라고 사료된다. The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Sus scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other animal species. There were 1,145 base pairs(bp) in the D-loop region. The middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specific sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified a 345 bp fragment, which contained the most variable region according to our sequencing data. SSCP analysis of denatured amplification products was carried out by polyacrylamide(8%) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polymorphism." @default.
- W2113488501 created "2016-06-24" @default.
- W2113488501 date "2003-12-31" @default.
- W2113488501 modified "2023-09-26" @default.
- W2113488501 title "Genetic Variation of Mitochondrial DNA in Duroc (Sus Scrofa) Using Single Stranded Conformation Polymorphism Analysis" @default.
- W2113488501 cites W1491713847 @default.
- W2113488501 cites W2010996608 @default.
- W2113488501 cites W2068740169 @default.
- W2113488501 cites W2068812148 @default.
- W2113488501 cites W2146535649 @default.
- W2113488501 cites W2155234008 @default.
- W2113488501 doi "https://doi.org/10.5187/jast.2003.45.6.911" @default.
- W2113488501 hasPublicationYear "2003" @default.
- W2113488501 type Work @default.
- W2113488501 sameAs 2113488501 @default.
- W2113488501 citedByCount "2" @default.
- W2113488501 countsByYear W21134885012023 @default.
- W2113488501 crossrefType "journal-article" @default.
- W2113488501 hasBestOaLocation W21134885011 @default.
- W2113488501 hasConcept C104317684 @default.
- W2113488501 hasConcept C129219603 @default.
- W2113488501 hasConcept C129312508 @default.
- W2113488501 hasConcept C153911025 @default.
- W2113488501 hasConcept C178790620 @default.
- W2113488501 hasConcept C185581394 @default.
- W2113488501 hasConcept C185592680 @default.
- W2113488501 hasConcept C198268994 @default.
- W2113488501 hasConcept C24586158 @default.
- W2113488501 hasConcept C2776907736 @default.
- W2113488501 hasConcept C2777563447 @default.
- W2113488501 hasConcept C49105822 @default.
- W2113488501 hasConcept C54355233 @default.
- W2113488501 hasConcept C552990157 @default.
- W2113488501 hasConcept C86803240 @default.
- W2113488501 hasConceptScore W2113488501C104317684 @default.
- W2113488501 hasConceptScore W2113488501C129219603 @default.
- W2113488501 hasConceptScore W2113488501C129312508 @default.
- W2113488501 hasConceptScore W2113488501C153911025 @default.
- W2113488501 hasConceptScore W2113488501C178790620 @default.
- W2113488501 hasConceptScore W2113488501C185581394 @default.
- W2113488501 hasConceptScore W2113488501C185592680 @default.
- W2113488501 hasConceptScore W2113488501C198268994 @default.
- W2113488501 hasConceptScore W2113488501C24586158 @default.
- W2113488501 hasConceptScore W2113488501C2776907736 @default.
- W2113488501 hasConceptScore W2113488501C2777563447 @default.
- W2113488501 hasConceptScore W2113488501C49105822 @default.
- W2113488501 hasConceptScore W2113488501C54355233 @default.
- W2113488501 hasConceptScore W2113488501C552990157 @default.
- W2113488501 hasConceptScore W2113488501C86803240 @default.
- W2113488501 hasIssue "6" @default.
- W2113488501 hasLocation W21134885011 @default.
- W2113488501 hasOpenAccess W2113488501 @default.
- W2113488501 hasPrimaryLocation W21134885011 @default.
- W2113488501 hasRelatedWork W102342276 @default.
- W2113488501 hasRelatedWork W1778528718 @default.
- W2113488501 hasRelatedWork W2015656314 @default.
- W2113488501 hasRelatedWork W2037912916 @default.
- W2113488501 hasRelatedWork W2047106367 @default.
- W2113488501 hasRelatedWork W2048825381 @default.
- W2113488501 hasRelatedWork W2113488501 @default.
- W2113488501 hasRelatedWork W2231629645 @default.
- W2113488501 hasRelatedWork W2416184527 @default.
- W2113488501 hasRelatedWork W2418630137 @default.
- W2113488501 hasVolume "45" @default.
- W2113488501 isParatext "false" @default.
- W2113488501 isRetracted "false" @default.
- W2113488501 magId "2113488501" @default.
- W2113488501 workType "article" @default.