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- W2113572083 abstract "Abstract An ultrasensitive and simple dynamic‐light‐scattering (DLS) assay for the sequence‐specific recognition of double‐stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5′‐TTTCTCTTCCTT‐ CTCTTC‐(T) 12 ‐SH‐3′ (Oligo 1) and 5′‐TTCTTTCTTTTCTTTTTC‐(T) 12 ‐ SH‐3′ (Oligo 2). The target dsDNA was composed of two complementary oligonucleotides: 5′‐AAAGAGAAGGAAGAGAAGAAGAAAGAAAAGAAAAAG‐3′ (Oligo 3) and 3′‐TTTCTCTTCCTTCTCTTCTTCTTTCTTTTCTTTTTC‐5′ (Oligo 4). Hybridization of the two AuNPs–Oligo probes with the target dsDNA induced aggregation of the target dsDNA by forming triplex DNA, which accordingly increased the average diameter. This diameter change could then be detected by DLS. The average diameter was proportional to the target dsDNA concentration over the range from 593 f M to 40 p M , with a detection limit of 593 f M . Moreover, the assay had good sequence specificity for the target dsDNA." @default.
- W2113572083 created "2016-06-24" @default.
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- W2113572083 date "2011-08-26" @default.
- W2113572083 modified "2023-10-18" @default.
- W2113572083 title "Dynamic-Light-Scattering-Based Sequence-Specific Recognition of Double-Stranded DNA with Oligonucleotide-Functionalized Gold Nanoparticles" @default.
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- W2113572083 doi "https://doi.org/10.1002/chem.201003010" @default.
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