FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113609462> ?p ?o ?g. }

• W2113609462 endingPage "547" @default.
• W2113609462 startingPage "537" @default.
• W2113609462 abstract "The Legionnaire's disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen which invades and replicates within two evolutionarily distant hosts, free-living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaire's disease. Although attachment and invasion of human macrophages by L. pneumophila is mediated in part by the complement receptors CR1 and CR3, the protozoan receptor involved in bacterial attachment and invasion has not been identified. To define the molecular events involved in invasion of protozoa by L. pneumophila, we examined the role of protein tyrosine phosphorylation of the protozoan host Hartmannella vermiformis upon attachment and invasion by L. pneumophila. Bacterial attachment and invasion were associated with a time-dependent tyrosine dephosphorylation of multiple host cell proteins. This host cell response was highly specific for live L. pneumophila, required contact with viable bacteria, and was completely reversible following washing off the bacteria from the host cell surface. Tyrosine dephosphorylation of host proteins was blocked by a tyrosine phosphatase inhibitor but not by tyrosine kinase inhibitors. One of the tyrosine dephosphorylated proteins was identified as the 170-kD galactose/N-acetylgalactosamine-inhibitable lectin (Gal/GalNAc) using immunoprecipitation and immunoblotting by antibodies generated against the Gal/GalNAc lectin of the protozoan Entamoeba histolytica. This Gal/GalNAc-inhibitable lectin has been shown previously to mediate adherence of E. histolytica to mammalian epithelial cells. Uptake of L. pneumophila by H. vermiformis was specifically inhibited by two monovalent sugars, Gal and GalNAc, and by mABs generated against the 170-kD lectin of E. histolytica. Interestingly, inhibition of invasion by Gal and GalNAc was associated with inhibition of bacterial-induced tyrosine dephosphorylation of H. vermiformis proteins. High stringency DNA hybridization confirmed the presence of the 170-kD lectin gene in H. vermiformis. We conclude that attachment of L. pneumophila to the H. vermiformis 170-kD lectin is required for invasion and is associated with tyrosine dephosphorylation of the Gal lectin and other host proteins. This is the first demonstration of a potential receptor used by L. pneumophila to invade protozoa." @default.
• W2113609462 created "2016-06-24" @default.
• W2113609462 creator A5007797366 @default.
• W2113609462 creator A5018120874 @default.
• W2113609462 creator A5059343866 @default.
• W2113609462 creator A5077316217 @default.
• W2113609462 date "1997-08-18" @default.
• W2113609462 modified "2023-09-26" @default.
• W2113609462 title "Identification of a Gal/GalNAc Lectin in the Protozoan <i>Hartmannella vermiformis</i> as a Potential Receptor for Attachment and Invasion by the Legionnaires' Disease Bacterium" @default.
• W2113609462 cites W1496030547 @default.
• W2113609462 cites W1583703772 @default.
• W2113609462 cites W1607418934 @default.
• W2113609462 cites W1900201061 @default.
• W2113609462 cites W1902000045 @default.
• W2113609462 cites W1964688185 @default.
• W2113609462 cites W1985689820 @default.
• W2113609462 cites W1994852714 @default.
• W2113609462 cites W1999924463 @default.
• W2113609462 cites W2002004699 @default.
• W2113609462 cites W2017526911 @default.
• W2113609462 cites W2019705373 @default.
• W2113609462 cites W2030024653 @default.
• W2113609462 cites W2030606199 @default.
• W2113609462 cites W2046428666 @default.
• W2113609462 cites W2047034243 @default.
• W2113609462 cites W2049706466 @default.
• W2113609462 cites W2054323226 @default.
• W2113609462 cites W2054728326 @default.
• W2113609462 cites W2057927518 @default.
• W2113609462 cites W2061102152 @default.
• W2113609462 cites W2066585170 @default.
• W2113609462 cites W2066630242 @default.
• W2113609462 cites W2073831807 @default.
• W2113609462 cites W2084257585 @default.
• W2113609462 cites W2090979926 @default.
• W2113609462 cites W2091444174 @default.
• W2113609462 cites W2104416424 @default.
• W2113609462 cites W2109460911 @default.
• W2113609462 cites W2110770741 @default.
• W2113609462 cites W2125213202 @default.
• W2113609462 cites W2140551159 @default.
• W2113609462 cites W2144591496 @default.
• W2113609462 cites W2145031990 @default.
• W2113609462 cites W2150967773 @default.
• W2113609462 cites W2151805768 @default.
• W2113609462 cites W2153356740 @default.
• W2113609462 cites W2159542202 @default.
• W2113609462 cites W2161459142 @default.
• W2113609462 cites W2163058312 @default.
• W2113609462 cites W2171716204 @default.
• W2113609462 cites W22628852 @default.
• W2113609462 cites W2267853604 @default.
• W2113609462 cites W2413756965 @default.
• W2113609462 cites W4236470188 @default.
• W2113609462 cites W4317527865 @default.
• W2113609462 doi "https://doi.org/10.1084/jem.186.4.537" @default.
• W2113609462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2199037" @default.
• W2113609462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9254652" @default.
• W2113609462 hasPublicationYear "1997" @default.
• W2113609462 type Work @default.
• W2113609462 sameAs 2113609462 @default.
• W2113609462 citedByCount "98" @default.
• W2113609462 countsByYear W21136094622012 @default.
• W2113609462 countsByYear W21136094622013 @default.
• W2113609462 countsByYear W21136094622014 @default.
• W2113609462 countsByYear W21136094622015 @default.
• W2113609462 countsByYear W21136094622016 @default.
• W2113609462 countsByYear W21136094622017 @default.
• W2113609462 countsByYear W21136094622018 @default.
• W2113609462 countsByYear W21136094622019 @default.
• W2113609462 countsByYear W21136094622020 @default.
• W2113609462 countsByYear W21136094622021 @default.
• W2113609462 crossrefType "journal-article" @default.
• W2113609462 hasAuthorship W2113609462A5007797366 @default.
• W2113609462 hasAuthorship W2113609462A5018120874 @default.
• W2113609462 hasAuthorship W2113609462A5059343866 @default.
• W2113609462 hasAuthorship W2113609462A5077316217 @default.
• W2113609462 hasBestOaLocation W21136094621 @default.
• W2113609462 hasConcept C153726182 @default.
• W2113609462 hasConcept C2776118409 @default.
• W2113609462 hasConcept C2776867272 @default.
• W2113609462 hasConcept C2777795018 @default.
• W2113609462 hasConcept C2778566189 @default.
• W2113609462 hasConcept C2778868110 @default.
• W2113609462 hasConcept C523546767 @default.
• W2113609462 hasConcept C54355233 @default.
• W2113609462 hasConcept C55493867 @default.
• W2113609462 hasConcept C79879829 @default.
• W2113609462 hasConcept C86803240 @default.
• W2113609462 hasConcept C89423630 @default.
• W2113609462 hasConceptScore W2113609462C153726182 @default.
• W2113609462 hasConceptScore W2113609462C2776118409 @default.
• W2113609462 hasConceptScore W2113609462C2776867272 @default.
• W2113609462 hasConceptScore W2113609462C2777795018 @default.
• W2113609462 hasConceptScore W2113609462C2778566189 @default.
• W2113609462 hasConceptScore W2113609462C2778868110 @default.
• W2113609462 hasConceptScore W2113609462C523546767 @default.
• W2113609462 hasConceptScore W2113609462C54355233 @default.

• next