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• W2113617534 abstract "Bursaphelenchus xylophilus (Steiner and Buhrer) and B. mucronatus (Mamiya and Enda), two closely related nematode parasites of conifers, were studied for the molecular, reproductive, behavioural and pathogenic characteristics which could be used in specific and subspecific identification. lntraspecific cross-hybridization among isolates of B. xylophilus produced fertile offspring. Crosshybridization between B. mucronatus from France (female) and Japan (male) produced fertile progeny but the reciprocal cross died out. Most interspecific hybrids between male B. xylophilus and female 8. mucronatus died out, but the reciprocal interspecific crosses were successful. Mortality of Pinus sylvestt-is seedlings was high after inoculation with B. xylophilus populations or their intraspecific hybrids, moderate after inoculation with the French B. mucronatus, low with the Japanese 0. mucronatus, and moderate with the 8. mucronatus Japanese-French intraspecific hybrid. Bursaphelenchus mucronatus and B. xylophilus interspecific hybrids were as pathogenic as the 8. xylophilus parents and more pathogenic than the 8. mucmnatrrs parents. No correlation was found between the female tail shape of B. xylophilus populations and the ability to cause pathogenicity. Genomic DNA from B. xylophilus, B. mucronatus and their hybrids was probed with a homologous ribosomal gene clone which produced band pattern differences between the populations of B. xylophilus and ctf B. mucronatus and between the French and Japanese populations of B. mucronatus. Males of B. xylophilus and B. mucronatus were attracted to pheromones released by their homospecific females. However, males or females of 5. mucronatus were not attracted to B. xylophilus females or males. Neither members of B. xylophilus nor 5. mucronatus were attracted to members of B. fraudulentus or Aphelenchoides rhyntium. Partial characterization of the pheromone of B. xylophilus revealed it to be a highly polar, hydrophilic compound. The results support the hypothesis that B. xylophilus and B. mucronatus are distinct species and provide additional evidence for the splitting of B. mucronatus into two species, one indigenous to France and the other to Japan. In addition, this study provides biological evidence that would lead to the re-evaluation of the embargo against the importation of North America softwood products into several European countries." @default.
• W2113617534 created "2016-06-24" @default.
• W2113617534 creator A5012148336 @default.
• W2113617534 date "1992-01-01" @default.
• W2113617534 modified "2023-09-27" @default.
• W2113617534 title "Multifaceted approach for differentiating isolates of Bursaphelenchus xylophilus and B. mucronatus (Nematoda), parasites of pine trees" @default.
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