Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113643089> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2113643089 endingPage "14853" @default.
- W2113643089 startingPage "14845" @default.
- W2113643089 abstract "Pyruvate phosphate dikinase (PPDK) catalyzes the reversible conversion of phosphoenolpyruvate (PEP), AMP, and Pi to pyruvate and ATP. The enzyme contains two remotely located reaction centers: the nucleotide partial reaction takes place at the N-terminal domain, and the PEP/pyruvate partial reaction takes place at the C-terminal domain. A central domain, tethered to the N- and C-terminal domains by two closely associated linkers, contains a phosphorylatable histidine residue (His455). The molecular architecture suggests a swiveling domain mechanism that shuttles a phosphoryl group between the two reaction centers. In an early structure of PPDK from Clostridium symbiosum, the His445-containing domain (His domain) was positioned close to the nucleotide binding domain and did not contact the PEP/pyruvate-binding domain. Here, we present the crystal structure of a second conformational state of C. symbiosum PPDK with the His domain adjacent to the PEP-binding domain. The structure was obtained by producing a three-residue mutant protein (R219E/E271R/S262D) that introduces repulsion between the His and nucleotide-binding domains but preserves viable interactions with the PEP/pyruvate-binding domain. Accordingly, the mutant enzyme is competent in catalyzing the PEP/pyruvate half-reaction but the overall activity is abolished. The new structure confirms the swivel motion of the His domain. In addition, upon detachment from the His domain, the two nucleotide-binding subdomains undergo a hinge motion that opens the active-site cleft. A similar hinge motion is expected to accompany nucleotide binding (cleft closure) and release (cleft opening). A model of the coupled swivel and cleft opening motions was generated by interpolation between two end conformations, each with His455 positioned for phosphoryl group transfer from/to one of the substrates. The trajectory of the His domain avoids major clashes with the partner domains while preserving the association of the two linker segments." @default.
- W2113643089 created "2016-06-24" @default.
- W2113643089 creator A5010988075 @default.
- W2113643089 creator A5043323040 @default.
- W2113643089 creator A5051943675 @default.
- W2113643089 creator A5068323278 @default.
- W2113643089 creator A5069488971 @default.
- W2113643089 creator A5072432337 @default.
- W2113643089 creator A5078623877 @default.
- W2113643089 creator A5081170581 @default.
- W2113643089 creator A5081453039 @default.
- W2113643089 date "2007-12-01" @default.
- W2113643089 modified "2023-10-14" @default.
- W2113643089 title "Swiveling Domain Mechanism in Pyruvate Phosphate Dikinase<sup>,</sup>" @default.
- W2113643089 cites W1564691977 @default.
- W2113643089 cites W1719996763 @default.
- W2113643089 cites W172079905 @default.
- W2113643089 cites W2028231353 @default.
- W2113643089 cites W2044082153 @default.
- W2113643089 cites W2050974639 @default.
- W2113643089 doi "https://doi.org/10.1021/bi701848w" @default.
- W2113643089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18052212" @default.
- W2113643089 hasPublicationYear "2007" @default.
- W2113643089 type Work @default.
- W2113643089 sameAs 2113643089 @default.
- W2113643089 citedByCount "31" @default.
- W2113643089 countsByYear W21136430892012 @default.
- W2113643089 countsByYear W21136430892013 @default.
- W2113643089 countsByYear W21136430892014 @default.
- W2113643089 countsByYear W21136430892016 @default.
- W2113643089 countsByYear W21136430892017 @default.
- W2113643089 countsByYear W21136430892018 @default.
- W2113643089 countsByYear W21136430892019 @default.
- W2113643089 countsByYear W21136430892020 @default.
- W2113643089 countsByYear W21136430892021 @default.
- W2113643089 countsByYear W21136430892022 @default.
- W2113643089 countsByYear W21136430892023 @default.
- W2113643089 crossrefType "journal-article" @default.
- W2113643089 hasAuthorship W2113643089A5010988075 @default.
- W2113643089 hasAuthorship W2113643089A5043323040 @default.
- W2113643089 hasAuthorship W2113643089A5051943675 @default.
- W2113643089 hasAuthorship W2113643089A5068323278 @default.
- W2113643089 hasAuthorship W2113643089A5069488971 @default.
- W2113643089 hasAuthorship W2113643089A5072432337 @default.
- W2113643089 hasAuthorship W2113643089A5078623877 @default.
- W2113643089 hasAuthorship W2113643089A5081170581 @default.
- W2113643089 hasAuthorship W2113643089A5081453039 @default.
- W2113643089 hasBestOaLocation W21136430892 @default.
- W2113643089 hasConcept C104317684 @default.
- W2113643089 hasConcept C10522644 @default.
- W2113643089 hasConcept C107824862 @default.
- W2113643089 hasConcept C113027372 @default.
- W2113643089 hasConcept C143065580 @default.
- W2113643089 hasConcept C143937172 @default.
- W2113643089 hasConcept C181199279 @default.
- W2113643089 hasConcept C185592680 @default.
- W2113643089 hasConcept C25166345 @default.
- W2113643089 hasConcept C2781338088 @default.
- W2113643089 hasConcept C47701112 @default.
- W2113643089 hasConcept C512185932 @default.
- W2113643089 hasConcept C55493867 @default.
- W2113643089 hasConcept C71240020 @default.
- W2113643089 hasConceptScore W2113643089C104317684 @default.
- W2113643089 hasConceptScore W2113643089C10522644 @default.
- W2113643089 hasConceptScore W2113643089C107824862 @default.
- W2113643089 hasConceptScore W2113643089C113027372 @default.
- W2113643089 hasConceptScore W2113643089C143065580 @default.
- W2113643089 hasConceptScore W2113643089C143937172 @default.
- W2113643089 hasConceptScore W2113643089C181199279 @default.
- W2113643089 hasConceptScore W2113643089C185592680 @default.
- W2113643089 hasConceptScore W2113643089C25166345 @default.
- W2113643089 hasConceptScore W2113643089C2781338088 @default.
- W2113643089 hasConceptScore W2113643089C47701112 @default.
- W2113643089 hasConceptScore W2113643089C512185932 @default.
- W2113643089 hasConceptScore W2113643089C55493867 @default.
- W2113643089 hasConceptScore W2113643089C71240020 @default.
- W2113643089 hasIssue "51" @default.
- W2113643089 hasLocation W21136430891 @default.
- W2113643089 hasLocation W21136430892 @default.
- W2113643089 hasLocation W21136430893 @default.
- W2113643089 hasOpenAccess W2113643089 @default.
- W2113643089 hasPrimaryLocation W21136430891 @default.
- W2113643089 hasRelatedWork W1974286739 @default.
- W2113643089 hasRelatedWork W1997683263 @default.
- W2113643089 hasRelatedWork W2014888463 @default.
- W2113643089 hasRelatedWork W2048261270 @default.
- W2113643089 hasRelatedWork W2054430652 @default.
- W2113643089 hasRelatedWork W2058227085 @default.
- W2113643089 hasRelatedWork W2113643089 @default.
- W2113643089 hasRelatedWork W2127253477 @default.
- W2113643089 hasRelatedWork W2309427866 @default.
- W2113643089 hasRelatedWork W2411475710 @default.
- W2113643089 hasVolume "46" @default.
- W2113643089 isParatext "false" @default.
- W2113643089 isRetracted "false" @default.
- W2113643089 magId "2113643089" @default.
- W2113643089 workType "article" @default.