Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113643753> ?p ?o ?g. }
- W2113643753 endingPage "1529" @default.
- W2113643753 startingPage "1524" @default.
- W2113643753 abstract "A protease was purified from Porphyromonas gingivalis 1101, a clinical isolate, by sequential sodium dodecyl sulfate-polyacrylamide gel electrophoresis, substrate diffusion gel electrophoresis, and electroelution. The enzyme cleaved radiolabeled human basement membrane type IV collagen and the synthetic collagen peptide substrate for eukaryotic collagenases. It was inactivated by the thiol protease inhibitor N-ethylmaleimide but not by EDTA or EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] and activated by reducing agents such as beta-mercaptoethanol. The enzyme exists as an active precursor protein of molecular mass 94 kDa and undergoes proteolytic cleavage to 75-, 56-, and 19-kDa forms. Biotin-labeled collagen bound specifically to the 94-kDa form of the protein and to its cleavage products in ligand blots, suggesting a role for this enzyme not only in collagen degradation but also in adhesion to collagenous substrata." @default.
- W2113643753 created "2016-06-24" @default.
- W2113643753 creator A5057195222 @default.
- W2113643753 creator A5060402346 @default.
- W2113643753 date "1992-04-01" @default.
- W2113643753 modified "2023-09-23" @default.
- W2113643753 title "Biochemical characterization of Porphyromonas (Bacteroides) gingivalis collagenase" @default.
- W2113643753 cites W1496217927 @default.
- W2113643753 cites W1501405625 @default.
- W2113643753 cites W1638453242 @default.
- W2113643753 cites W1954470537 @default.
- W2113643753 cites W1957749435 @default.
- W2113643753 cites W1980640496 @default.
- W2113643753 cites W2000790125 @default.
- W2113643753 cites W2003250023 @default.
- W2113643753 cites W2005836838 @default.
- W2113643753 cites W2029034486 @default.
- W2113643753 cites W2029555146 @default.
- W2113643753 cites W2031592714 @default.
- W2113643753 cites W2054835887 @default.
- W2113643753 cites W2068593367 @default.
- W2113643753 cites W2080363074 @default.
- W2113643753 cites W2089177065 @default.
- W2113643753 cites W2094749758 @default.
- W2113643753 cites W2101563601 @default.
- W2113643753 cites W2119698715 @default.
- W2113643753 cites W2120316045 @default.
- W2113643753 cites W2141695328 @default.
- W2113643753 cites W2165947300 @default.
- W2113643753 cites W2272109980 @default.
- W2113643753 cites W2273652525 @default.
- W2113643753 cites W2321092366 @default.
- W2113643753 doi "https://doi.org/10.1128/iai.60.4.1524-1529.1992" @default.
- W2113643753 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/257026" @default.
- W2113643753 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1312517" @default.
- W2113643753 hasPublicationYear "1992" @default.
- W2113643753 type Work @default.
- W2113643753 sameAs 2113643753 @default.
- W2113643753 citedByCount "50" @default.
- W2113643753 countsByYear W21136437532014 @default.
- W2113643753 countsByYear W21136437532015 @default.
- W2113643753 countsByYear W21136437532016 @default.
- W2113643753 countsByYear W21136437532017 @default.
- W2113643753 crossrefType "journal-article" @default.
- W2113643753 hasAuthorship W2113643753A5057195222 @default.
- W2113643753 hasAuthorship W2113643753A5060402346 @default.
- W2113643753 hasBestOaLocation W21136437531 @default.
- W2113643753 hasConcept C101660505 @default.
- W2113643753 hasConcept C116084860 @default.
- W2113643753 hasConcept C153911025 @default.
- W2113643753 hasConcept C178790620 @default.
- W2113643753 hasConcept C181199279 @default.
- W2113643753 hasConcept C185592680 @default.
- W2113643753 hasConcept C2776714187 @default.
- W2113643753 hasConcept C2777658833 @default.
- W2113643753 hasConcept C2778394429 @default.
- W2113643753 hasConcept C2780183776 @default.
- W2113643753 hasConcept C519063684 @default.
- W2113643753 hasConcept C523546767 @default.
- W2113643753 hasConcept C54355233 @default.
- W2113643753 hasConcept C55493867 @default.
- W2113643753 hasConcept C55728118 @default.
- W2113643753 hasConcept C86803240 @default.
- W2113643753 hasConceptScore W2113643753C101660505 @default.
- W2113643753 hasConceptScore W2113643753C116084860 @default.
- W2113643753 hasConceptScore W2113643753C153911025 @default.
- W2113643753 hasConceptScore W2113643753C178790620 @default.
- W2113643753 hasConceptScore W2113643753C181199279 @default.
- W2113643753 hasConceptScore W2113643753C185592680 @default.
- W2113643753 hasConceptScore W2113643753C2776714187 @default.
- W2113643753 hasConceptScore W2113643753C2777658833 @default.
- W2113643753 hasConceptScore W2113643753C2778394429 @default.
- W2113643753 hasConceptScore W2113643753C2780183776 @default.
- W2113643753 hasConceptScore W2113643753C519063684 @default.
- W2113643753 hasConceptScore W2113643753C523546767 @default.
- W2113643753 hasConceptScore W2113643753C54355233 @default.
- W2113643753 hasConceptScore W2113643753C55493867 @default.
- W2113643753 hasConceptScore W2113643753C55728118 @default.
- W2113643753 hasConceptScore W2113643753C86803240 @default.
- W2113643753 hasIssue "4" @default.
- W2113643753 hasLocation W21136437531 @default.
- W2113643753 hasLocation W21136437532 @default.
- W2113643753 hasLocation W21136437533 @default.
- W2113643753 hasLocation W21136437534 @default.
- W2113643753 hasOpenAccess W2113643753 @default.
- W2113643753 hasPrimaryLocation W21136437531 @default.
- W2113643753 hasRelatedWork W130188589 @default.
- W2113643753 hasRelatedWork W1440031586 @default.
- W2113643753 hasRelatedWork W1808233456 @default.
- W2113643753 hasRelatedWork W1821270395 @default.
- W2113643753 hasRelatedWork W2052067084 @default.
- W2113643753 hasRelatedWork W2074738418 @default.
- W2113643753 hasRelatedWork W2109078573 @default.
- W2113643753 hasRelatedWork W2113643753 @default.
- W2113643753 hasRelatedWork W2163536072 @default.
- W2113643753 hasRelatedWork W2463802760 @default.
- W2113643753 hasVolume "60" @default.
- W2113643753 isParatext "false" @default.