FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113647940> ?p ?o ?g. }

• W2113647940 endingPage "328" @default.
• W2113647940 startingPage "320" @default.
• W2113647940 abstract "ABSTRACT Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1 , ORF2 , and ORF3 . Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. Using the yeast three-hybrid system and traditional biochemical techniques, we have studied the RNA binding activities of ORF2 and ORF3, two proteins encoded in the 3′ structural part of the genome. Since the genomic RNA from HEV has been postulated to contain secondary structures at the 5′ and 3′ ends, we used these two terminal regions, besides other regions within the genome, in this study. Experiments were designed to test for interactions between the genomic RNA fusion constructs with ORF2 and ORF3 hybrid proteins in a yeast cellular environment. We show here that the ORF2 protein contains RNA binding activity. The ORF2 protein specifically bound the 5′ end of the HEV genome. Deletion analysis of this protein showed that its RNA binding activity was lost when deletions were made beyond the N-terminal 111 amino acids. Finer mapping of the interacting RNA revealed that a 76-nucleotide (nt) region at the 5′ end of the HEV genome was responsible for binding the ORF2 protein. This 76-nt region included the 51-nt HEV sequence, conserved across alphaviruses. Our results support the requirement of this conserved sequence for interaction with ORF2 and also indicate an increase in the strength of the RNA-protein interaction when an additional 44 bases downstream of this 76-nt region were included. Secondary-structure predictions and the location of the ORF2 binding region within the HEV genome indicate that this interaction may play a role in viral encapsidation." @default.
• W2113647940 created "2016-06-24" @default.
• W2113647940 creator A5022170940 @default.
• W2113647940 creator A5031518835 @default.
• W2113647940 creator A5076248093 @default.
• W2113647940 date "2004-01-01" @default.
• W2113647940 modified "2023-10-17" @default.
• W2113647940 title "The ORF2 Protein of Hepatitis E Virus Binds the 5′ Region of Viral RNA" @default.
• W2113647940 cites W1542686183 @default.
• W2113647940 cites W1566478858 @default.
• W2113647940 cites W1585874678 @default.
• W2113647940 cites W1695296338 @default.
• W2113647940 cites W1938014745 @default.
• W2113647940 cites W1962250011 @default.
• W2113647940 cites W1967776406 @default.
• W2113647940 cites W1972437755 @default.
• W2113647940 cites W1986405219 @default.
• W2113647940 cites W1987780425 @default.
• W2113647940 cites W1987962702 @default.
• W2113647940 cites W1996017311 @default.
• W2113647940 cites W2004249771 @default.
• W2113647940 cites W2006167038 @default.
• W2113647940 cites W2016461161 @default.
• W2113647940 cites W2017894098 @default.
• W2113647940 cites W2032567331 @default.
• W2113647940 cites W2043422501 @default.
• W2113647940 cites W2050838285 @default.
• W2113647940 cites W2052108496 @default.
• W2113647940 cites W2071827904 @default.
• W2113647940 cites W2072594662 @default.
• W2113647940 cites W2075666854 @default.
• W2113647940 cites W2076969999 @default.
• W2113647940 cites W2086330724 @default.
• W2113647940 cites W2100435038 @default.
• W2113647940 cites W2111246062 @default.
• W2113647940 cites W2123792518 @default.
• W2113647940 cites W2130666752 @default.
• W2113647940 cites W2135793835 @default.
• W2113647940 cites W2154627118 @default.
• W2113647940 cites W2167183394 @default.
• W2113647940 cites W2207950654 @default.
• W2113647940 cites W2224063625 @default.
• W2113647940 doi "https://doi.org/10.1128/jvi.78.1.320-328.2004" @default.
• W2113647940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/303377" @default.
• W2113647940 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14671114" @default.
• W2113647940 hasPublicationYear "2004" @default.
• W2113647940 type Work @default.
• W2113647940 sameAs 2113647940 @default.
• W2113647940 citedByCount "86" @default.
• W2113647940 countsByYear W21136479402012 @default.
• W2113647940 countsByYear W21136479402013 @default.
• W2113647940 countsByYear W21136479402015 @default.
• W2113647940 countsByYear W21136479402016 @default.
• W2113647940 countsByYear W21136479402017 @default.
• W2113647940 countsByYear W21136479402018 @default.
• W2113647940 countsByYear W21136479402019 @default.
• W2113647940 countsByYear W21136479402020 @default.
• W2113647940 countsByYear W21136479402021 @default.
• W2113647940 countsByYear W21136479402022 @default.
• W2113647940 countsByYear W21136479402023 @default.
• W2113647940 crossrefType "journal-article" @default.
• W2113647940 hasAuthorship W2113647940A5022170940 @default.
• W2113647940 hasAuthorship W2113647940A5031518835 @default.
• W2113647940 hasAuthorship W2113647940A5076248093 @default.
• W2113647940 hasBestOaLocation W21136479401 @default.
• W2113647940 hasConcept C104317684 @default.
• W2113647940 hasConcept C135763542 @default.
• W2113647940 hasConcept C141231307 @default.
• W2113647940 hasConcept C142575336 @default.
• W2113647940 hasConcept C153911025 @default.
• W2113647940 hasConcept C159047783 @default.
• W2113647940 hasConcept C167625842 @default.
• W2113647940 hasConcept C202878990 @default.
• W2113647940 hasConcept C2779174946 @default.
• W2113647940 hasConcept C2780777367 @default.
• W2113647940 hasConcept C41258723 @default.
• W2113647940 hasConcept C47289529 @default.
• W2113647940 hasConcept C54355233 @default.
• W2113647940 hasConcept C67705224 @default.
• W2113647940 hasConcept C86803240 @default.
• W2113647940 hasConceptScore W2113647940C104317684 @default.
• W2113647940 hasConceptScore W2113647940C135763542 @default.
• W2113647940 hasConceptScore W2113647940C141231307 @default.
• W2113647940 hasConceptScore W2113647940C142575336 @default.
• W2113647940 hasConceptScore W2113647940C153911025 @default.
• W2113647940 hasConceptScore W2113647940C159047783 @default.
• W2113647940 hasConceptScore W2113647940C167625842 @default.
• W2113647940 hasConceptScore W2113647940C202878990 @default.
• W2113647940 hasConceptScore W2113647940C2779174946 @default.
• W2113647940 hasConceptScore W2113647940C2780777367 @default.
• W2113647940 hasConceptScore W2113647940C41258723 @default.
• W2113647940 hasConceptScore W2113647940C47289529 @default.
• W2113647940 hasConceptScore W2113647940C54355233 @default.
• W2113647940 hasConceptScore W2113647940C67705224 @default.
• W2113647940 hasConceptScore W2113647940C86803240 @default.
• W2113647940 hasIssue "1" @default.
• W2113647940 hasLocation W21136479401 @default.
• W2113647940 hasLocation W21136479402 @default.

• next