FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113670484> ?p ?o ?g. }

• W2113670484 endingPage "93" @default.
• W2113670484 startingPage "85" @default.
• W2113670484 abstract "Studies in nonhuman primates offer information of high relevance to clinical medicine due to their close genetic relationship with humans. Here, we established an optimized protocol for the isolation of antibody V(D)J sequences from rhesus macaque B cells. Nested PCR primers were designed to align to sequences flanking the V(D)J coding region to enable amplification of highly mutated antibody sequences. The primers were evaluated using cDNA from bulk PBMCs as well as from single-sorted memory and naïve B cells from several macaques to ascertain effective germline coverage. The nested PCR efficiency reached 60.6% positive wells for heavy chain amplification, 39.2% for kappa chain, and 23.7% for lambda chain sequences. Matching heavy and light chain sequences, indicating antibodies that potentially can be cloned, were obtained in 50% of the positive wells. Using these primers, we found that the efficiency and specificity of V(D)J amplifications were markedly improved compared to when primers designed for human Ab isolation were used. In particular, the amplification of recombined light chain VJ sequences was improved. Thus, we describe the design and testing of a new set of rhesus-specific primers that enable efficient and specific amplification of heavy, kappa and lambda V(D)J genes from single sorted B cells. The use of these primers will facilitate future efforts to clone and express rhesus macaque MAbs for genetic, functional and structural analyses." @default.
• W2113670484 created "2016-06-24" @default.
• W2113670484 creator A5011139836 @default.
• W2113670484 creator A5024029113 @default.
• W2113670484 creator A5025713007 @default.
• W2113670484 creator A5035302832 @default.
• W2113670484 creator A5083386185 @default.
• W2113670484 date "2012-12-01" @default.
• W2113670484 modified "2023-10-16" @default.
• W2113670484 title "Isolation of antibody V(D)J sequences from single cell sorted rhesus macaque B cells" @default.
• W2113670484 cites W1509449144 @default.
• W2113670484 cites W1529109939 @default.
• W2113670484 cites W1748392249 @default.
• W2113670484 cites W1963778872 @default.
• W2113670484 cites W1968418560 @default.
• W2113670484 cites W1972143601 @default.
• W2113670484 cites W1977323163 @default.
• W2113670484 cites W1979886683 @default.
• W2113670484 cites W1989048471 @default.
• W2113670484 cites W1994878072 @default.
• W2113670484 cites W1995414211 @default.
• W2113670484 cites W2004798786 @default.
• W2113670484 cites W2011136801 @default.
• W2113670484 cites W2014081607 @default.
• W2113670484 cites W2018123062 @default.
• W2113670484 cites W2022486030 @default.
• W2113670484 cites W2029023023 @default.
• W2113670484 cites W2059430913 @default.
• W2113670484 cites W2068328204 @default.
• W2113670484 cites W2074806617 @default.
• W2113670484 cites W2076198413 @default.
• W2113670484 cites W2077679863 @default.
• W2113670484 cites W2081984206 @default.
• W2113670484 cites W2087470599 @default.
• W2113670484 cites W2089948278 @default.
• W2113670484 cites W2094761185 @default.
• W2113670484 cites W2099391799 @default.
• W2113670484 cites W2100749074 @default.
• W2113670484 cites W2103147884 @default.
• W2113670484 cites W2118274231 @default.
• W2113670484 cites W2119779320 @default.
• W2113670484 cites W2123708888 @default.
• W2113670484 cites W2125548284 @default.
• W2113670484 cites W2134087536 @default.
• W2113670484 cites W2135151522 @default.
• W2113670484 cites W2135792930 @default.
• W2113670484 cites W2144015945 @default.
• W2113670484 cites W2150090112 @default.
• W2113670484 cites W2156381249 @default.
• W2113670484 cites W2157552377 @default.
• W2113670484 cites W2169156878 @default.
• W2113670484 cites W2171690009 @default.
• W2113670484 cites W2578150941 @default.
• W2113670484 doi "https://doi.org/10.1016/j.jim.2012.09.003" @default.
• W2113670484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22989932" @default.
• W2113670484 hasPublicationYear "2012" @default.
• W2113670484 type Work @default.
• W2113670484 sameAs 2113670484 @default.
• W2113670484 citedByCount "68" @default.
• W2113670484 countsByYear W21136704842014 @default.
• W2113670484 countsByYear W21136704842015 @default.
• W2113670484 countsByYear W21136704842016 @default.
• W2113670484 countsByYear W21136704842017 @default.
• W2113670484 countsByYear W21136704842018 @default.
• W2113670484 countsByYear W21136704842019 @default.
• W2113670484 countsByYear W21136704842020 @default.
• W2113670484 countsByYear W21136704842021 @default.
• W2113670484 countsByYear W21136704842022 @default.
• W2113670484 countsByYear W21136704842023 @default.
• W2113670484 crossrefType "journal-article" @default.
• W2113670484 hasAuthorship W2113670484A5011139836 @default.
• W2113670484 hasAuthorship W2113670484A5024029113 @default.
• W2113670484 hasAuthorship W2113670484A5025713007 @default.
• W2113670484 hasAuthorship W2113670484A5035302832 @default.
• W2113670484 hasAuthorship W2113670484A5083386185 @default.
• W2113670484 hasConcept C104317684 @default.
• W2113670484 hasConcept C138885662 @default.
• W2113670484 hasConcept C151730666 @default.
• W2113670484 hasConcept C153911025 @default.
• W2113670484 hasConcept C159047783 @default.
• W2113670484 hasConcept C159654299 @default.
• W2113670484 hasConcept C187882448 @default.
• W2113670484 hasConcept C2778724333 @default.
• W2113670484 hasConcept C2778950215 @default.
• W2113670484 hasConcept C2779175743 @default.
• W2113670484 hasConcept C36394416 @default.
• W2113670484 hasConcept C41895202 @default.
• W2113670484 hasConcept C49105822 @default.
• W2113670484 hasConcept C54355233 @default.
• W2113670484 hasConcept C70721500 @default.
• W2113670484 hasConcept C81089528 @default.
• W2113670484 hasConcept C86803240 @default.
• W2113670484 hasConceptScore W2113670484C104317684 @default.
• W2113670484 hasConceptScore W2113670484C138885662 @default.
• W2113670484 hasConceptScore W2113670484C151730666 @default.
• W2113670484 hasConceptScore W2113670484C153911025 @default.
• W2113670484 hasConceptScore W2113670484C159047783 @default.
• W2113670484 hasConceptScore W2113670484C159654299 @default.

• next