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- W2113699372 abstract "Celiac disease (CD) is an autoimmune disease characterized by chronic diarrhea, inflammatory lesions of small bowel and nutritional malabsorption. CD is strongly associated with the presence of HLA-DQB*02, DQB*03:02 and DQA*05. The absence of any one of these three human leukocyte antigen (HLA) alleles rules out the diagnosis of CD in suspected patients. Here, we describe a novel method to detect the presence of these specific HLA alleles using real-time polymerase chain reaction (PCR) with melting curve analysis. Compared with current HLA typing assays, the real-time PCR method is faster, requires fewer handling steps and provides 100% sensitivity and specificity for typing of HLA-DQB*02, DQB*03:02 and DQA*05 alleles." @default.
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- W2113699372 date "2011-04-27" @default.
- W2113699372 modified "2023-09-23" @default.
- W2113699372 title "Celiac disease and HLA typing using real-time PCR with melting curve analysis" @default.
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- W2113699372 doi "https://doi.org/10.1111/j.1399-0039.2011.01676.x" @default.
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