FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113707210> ?p ?o ?g. }

• W2113707210 endingPage "984" @default.
• W2113707210 startingPage "976" @default.
• W2113707210 abstract "Abstract Hydrogel membranes have been fabricated that incorporate antibody/antigen moieties. The permeability of large solutes through these membranes is dependent on the presence of soluble antigen that can compete with the internal interactions between antibody and antigen leading to an increase in gel mesh size. Specifically, the membrane's structure is based on a dextran backbone grafted with a fluorescein isothiocyanate (FITC) antigen and a sheep anti‐FITC IgG antibody. The backbone is covalently cross‐linked by conjugated divinyl sulfone (DVS) groups. The gel structure is additionally stabilized by affinity crosslinks formed by biospecific interactions between the bound IgG and FITC. FTIR spectra of the gel are consistent with formation of covalent bonds between cysteine groups in the IgG and DVS groups in the dextran. Results obtained using isothermal titration calorimetry (ITC) confirmed the competitive interaction binding between IgG‐FITC‐dextran and free sodium fluorescein at pH 5.0. Scanning electron microscopy (SEM) of samples prepared using cryofixation and cryofracturing techniques showed that observed changes in permeability correlate with free fluorescein‐dependent structural changes in the gel. Three‐dimensional images obtained from confocal laser scanning microscopy show that these changes occur throughout the gel and indicate that SEM results are not artifacts of sample preparation. The permeability of these gels, as shown by blue‐dextran (12 kDa) diffusion, increases in response to the presence of free fluorescein of the external medium, which causes competitive displacement of the affinity cross‐links. Sequential addition and removal of sodium fluorescein showed that these permeability changes are reversible. Biotechnol. Bioeng. 2007;97: 976–984. © 2006 Wiley Periodicals, Inc." @default.
• W2113707210 created "2016-06-24" @default.
• W2113707210 creator A5018408488 @default.
• W2113707210 creator A5026380333 @default.
• W2113707210 creator A5028871966 @default.
• W2113707210 creator A5073696107 @default.
• W2113707210 date "2007-05-24" @default.
• W2113707210 modified "2023-10-15" @default.
• W2113707210 title "A smart membrane based on an antigen-responsive hydrogel" @default.
• W2113707210 cites W1507777757 @default.
• W2113707210 cites W1515338173 @default.
• W2113707210 cites W1964577763 @default.
• W2113707210 cites W1970141580 @default.
• W2113707210 cites W1995093464 @default.
• W2113707210 cites W2005454227 @default.
• W2113707210 cites W2014527656 @default.
• W2113707210 cites W2022159326 @default.
• W2113707210 cites W2031525159 @default.
• W2113707210 cites W2043325430 @default.
• W2113707210 cites W2048938205 @default.
• W2113707210 cites W2064523452 @default.
• W2113707210 cites W2073654270 @default.
• W2113707210 cites W2083576488 @default.
• W2113707210 cites W2089609747 @default.
• W2113707210 cites W2092189912 @default.
• W2113707210 cites W2100501295 @default.
• W2113707210 cites W2109782053 @default.
• W2113707210 cites W2143558167 @default.
• W2113707210 cites W2317012207 @default.
• W2113707210 cites W4211166201 @default.
• W2113707210 doi "https://doi.org/10.1002/bit.21255" @default.
• W2113707210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17149770" @default.
• W2113707210 hasPublicationYear "2007" @default.
• W2113707210 type Work @default.
• W2113707210 sameAs 2113707210 @default.
• W2113707210 citedByCount "49" @default.
• W2113707210 countsByYear W21137072102012 @default.
• W2113707210 countsByYear W21137072102013 @default.
• W2113707210 countsByYear W21137072102014 @default.
• W2113707210 countsByYear W21137072102015 @default.
• W2113707210 countsByYear W21137072102016 @default.
• W2113707210 countsByYear W21137072102017 @default.
• W2113707210 countsByYear W21137072102018 @default.
• W2113707210 countsByYear W21137072102019 @default.
• W2113707210 countsByYear W21137072102020 @default.
• W2113707210 countsByYear W21137072102021 @default.
• W2113707210 countsByYear W21137072102022 @default.
• W2113707210 crossrefType "journal-article" @default.
• W2113707210 hasAuthorship W2113707210A5018408488 @default.
• W2113707210 hasAuthorship W2113707210A5026380333 @default.
• W2113707210 hasAuthorship W2113707210A5028871966 @default.
• W2113707210 hasAuthorship W2113707210A5073696107 @default.
• W2113707210 hasConcept C121332964 @default.
• W2113707210 hasConcept C12554922 @default.
• W2113707210 hasConcept C156911925 @default.
• W2113707210 hasConcept C178790620 @default.
• W2113707210 hasConcept C180577832 @default.
• W2113707210 hasConcept C185592680 @default.
• W2113707210 hasConcept C2777019933 @default.
• W2113707210 hasConcept C2777339698 @default.
• W2113707210 hasConcept C2778015030 @default.
• W2113707210 hasConcept C2779178360 @default.
• W2113707210 hasConcept C41625074 @default.
• W2113707210 hasConcept C43617362 @default.
• W2113707210 hasConcept C55493867 @default.
• W2113707210 hasConcept C62520636 @default.
• W2113707210 hasConcept C86803240 @default.
• W2113707210 hasConcept C91881484 @default.
• W2113707210 hasConcept C95444343 @default.
• W2113707210 hasConceptScore W2113707210C121332964 @default.
• W2113707210 hasConceptScore W2113707210C12554922 @default.
• W2113707210 hasConceptScore W2113707210C156911925 @default.
• W2113707210 hasConceptScore W2113707210C178790620 @default.
• W2113707210 hasConceptScore W2113707210C180577832 @default.
• W2113707210 hasConceptScore W2113707210C185592680 @default.
• W2113707210 hasConceptScore W2113707210C2777019933 @default.
• W2113707210 hasConceptScore W2113707210C2777339698 @default.
• W2113707210 hasConceptScore W2113707210C2778015030 @default.
• W2113707210 hasConceptScore W2113707210C2779178360 @default.
• W2113707210 hasConceptScore W2113707210C41625074 @default.
• W2113707210 hasConceptScore W2113707210C43617362 @default.
• W2113707210 hasConceptScore W2113707210C55493867 @default.
• W2113707210 hasConceptScore W2113707210C62520636 @default.
• W2113707210 hasConceptScore W2113707210C86803240 @default.
• W2113707210 hasConceptScore W2113707210C91881484 @default.
• W2113707210 hasConceptScore W2113707210C95444343 @default.
• W2113707210 hasIssue "4" @default.
• W2113707210 hasLocation W21137072101 @default.
• W2113707210 hasLocation W21137072102 @default.
• W2113707210 hasOpenAccess W2113707210 @default.
• W2113707210 hasPrimaryLocation W21137072101 @default.
• W2113707210 hasRelatedWork W1998987725 @default.
• W2113707210 hasRelatedWork W2001008105 @default.
• W2113707210 hasRelatedWork W2032816982 @default.
• W2113707210 hasRelatedWork W2033193375 @default.
• W2113707210 hasRelatedWork W2034796571 @default.
• W2113707210 hasRelatedWork W2050723662 @default.
• W2113707210 hasRelatedWork W2092718165 @default.

• next