Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113753551> ?p ?o ?g. }
- W2113753551 endingPage "5326" @default.
- W2113753551 startingPage "5309" @default.
- W2113753551 abstract "The independently folded appendages of the large alpha and beta2 subunits of the endocytic adaptor protein (AP)-2 complex coordinate proper assembly and operation of endocytic components during clathrin-mediated endocytosis. The beta2 subunit appendage contains a common binding site for beta-arrestin or the autosomal recessive hypercholesterolemia (ARH) protein. To determine the importance of this interaction surface in living cells, we used small interfering RNA-based gene silencing. The effect of extinguishing beta2 subunit expression on the internalization of transferrin is considerably weaker than an AP-2 alpha subunit knockdown. We show the mild sorting defect is due to fortuitous substitution of the beta2 chain with the closely related endogenous beta1 subunit of the AP-1 adaptor complex. Simultaneous silencing of both beta1 and beta2 subunit transcripts recapitulates the strong alpha subunit RNA interference (RNAi) phenotype and results in loss of ARH from endocytic clathrin coats. An RNAi-insensitive beta2-yellow fluorescent protein (YFP) expressed in the beta1 + beta2-silenced background restores cellular AP-2 levels, robust transferrin internalization, and ARH colocalization with cell surface clathrin. The importance of the beta appendage platform subdomain over clathrin for precise deposition of ARH at clathrin assembly zones is revealed by a beta2-YFP with a disrupted ARH binding interface, which does not restore ARH colocalization with clathrin. We also show a beta-arrestin 1 mutant, which engages coated structures in the absence of any G protein-coupled receptor stimulation, colocalizes with beta2-YFP and clathrin even in the absence of an operational clathrin binding sequence. These findings argue against ARH and beta-arrestin binding to a site upon the beta2 appendage platform that is later obstructed by polymerized clathrin. We conclude that ARH and beta-arrestin depend on a privileged beta2 appendage site for proper cargo recruitment to clathrin bud sites." @default.
- W2113753551 created "2016-06-24" @default.
- W2113753551 creator A5016759211 @default.
- W2113753551 creator A5029375173 @default.
- W2113753551 creator A5067234338 @default.
- W2113753551 creator A5069522130 @default.
- W2113753551 creator A5078781783 @default.
- W2113753551 creator A5080745671 @default.
- W2113753551 creator A5088924932 @default.
- W2113753551 creator A5091165788 @default.
- W2113753551 date "2008-12-01" @default.
- W2113753551 modified "2023-10-18" @default.
- W2113753551 title "The AP-2 Adaptor β2 Appendage Scaffolds Alternate Cargo Endocytosis" @default.
- W2113753551 cites W1505938433 @default.
- W2113753551 cites W1508211596 @default.
- W2113753551 cites W1543492257 @default.
- W2113753551 cites W1544762103 @default.
- W2113753551 cites W1565704598 @default.
- W2113753551 cites W1608183728 @default.
- W2113753551 cites W1611419331 @default.
- W2113753551 cites W1727091879 @default.
- W2113753551 cites W1857186759 @default.
- W2113753551 cites W1965139127 @default.
- W2113753551 cites W1970268623 @default.
- W2113753551 cites W1978139260 @default.
- W2113753551 cites W1979815209 @default.
- W2113753551 cites W1980740566 @default.
- W2113753551 cites W1982750125 @default.
- W2113753551 cites W1983921682 @default.
- W2113753551 cites W1985919575 @default.
- W2113753551 cites W1988113468 @default.
- W2113753551 cites W1988518158 @default.
- W2113753551 cites W1992257690 @default.
- W2113753551 cites W1993054972 @default.
- W2113753551 cites W1993355802 @default.
- W2113753551 cites W1996107769 @default.
- W2113753551 cites W1997140657 @default.
- W2113753551 cites W1998177121 @default.
- W2113753551 cites W2011226078 @default.
- W2113753551 cites W2011393081 @default.
- W2113753551 cites W2012107438 @default.
- W2113753551 cites W2013316045 @default.
- W2113753551 cites W2019196882 @default.
- W2113753551 cites W2020331445 @default.
- W2113753551 cites W2023651588 @default.
- W2113753551 cites W2025533401 @default.
- W2113753551 cites W2025765361 @default.
- W2113753551 cites W2025957324 @default.
- W2113753551 cites W2027030905 @default.
- W2113753551 cites W2029046578 @default.
- W2113753551 cites W2029234218 @default.
- W2113753551 cites W2029241563 @default.
- W2113753551 cites W2029675956 @default.
- W2113753551 cites W2029873308 @default.
- W2113753551 cites W2030222651 @default.
- W2113753551 cites W2030958832 @default.
- W2113753551 cites W2032444338 @default.
- W2113753551 cites W2032554631 @default.
- W2113753551 cites W2032675576 @default.
- W2113753551 cites W2033165803 @default.
- W2113753551 cites W2034072755 @default.
- W2113753551 cites W2037204799 @default.
- W2113753551 cites W2037386165 @default.
- W2113753551 cites W2038330825 @default.
- W2113753551 cites W2039023142 @default.
- W2113753551 cites W2042195196 @default.
- W2113753551 cites W2042238592 @default.
- W2113753551 cites W2048451267 @default.
- W2113753551 cites W2048973111 @default.
- W2113753551 cites W2049419164 @default.
- W2113753551 cites W2049741610 @default.
- W2113753551 cites W2050251450 @default.
- W2113753551 cites W2052288973 @default.
- W2113753551 cites W2053031822 @default.
- W2113753551 cites W2055231253 @default.
- W2113753551 cites W2055990292 @default.
- W2113753551 cites W2061457302 @default.
- W2113753551 cites W2068581450 @default.
- W2113753551 cites W2069177009 @default.
- W2113753551 cites W2071636548 @default.
- W2113753551 cites W2071725693 @default.
- W2113753551 cites W2074571847 @default.
- W2113753551 cites W2077053416 @default.
- W2113753551 cites W2078269238 @default.
- W2113753551 cites W2080104036 @default.
- W2113753551 cites W2086515708 @default.
- W2113753551 cites W2093994138 @default.
- W2113753551 cites W2096154351 @default.
- W2113753551 cites W2096807425 @default.
- W2113753551 cites W2097856882 @default.
- W2113753551 cites W2103481821 @default.
- W2113753551 cites W2107292352 @default.
- W2113753551 cites W2108328973 @default.
- W2113753551 cites W2108836535 @default.
- W2113753551 cites W2113438288 @default.
- W2113753551 cites W2115734610 @default.
- W2113753551 cites W2115977533 @default.
- W2113753551 cites W2115994196 @default.