Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113761719> ?p ?o ?g. }
- W2113761719 endingPage "144" @default.
- W2113761719 startingPage "137" @default.
- W2113761719 abstract "Background Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. Major emphasis has so far been directed at improving gene transfer efficiency, but low gene marking in transplanted recipients might equally well reflect compromised repopulating activity of transduced cells, competing for reconstitution with endogenous and unmanipulated stem cells. Methods The autologous settings of clinical gene therapy protocols preclude evaluation of changes in repopulating ability following transduction; however, using a congenic mouse model, allowing for direct evaluation of gene marking of lympho-myeloid progeny, we show here that these issues can be accurately addressed. Results We demonstrate that conditions supporting in vitro stem cell self-renewal efficiently promote oncoretroviral-mediated gene transfer to multipotent adult bone marrow stem cells, without prior in vivo conditioning. Despite using optimized culture conditions, transduction resulted in striking losses of repopulating activity, translating into low numbers of gene marked cells in competitively repopulated mice. Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. Conclusions These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells. Copyright © 2004 John Wiley & Sons, Ltd." @default.
- W2113761719 created "2016-06-24" @default.
- W2113761719 creator A5003918131 @default.
- W2113761719 creator A5041041189 @default.
- W2113761719 creator A5046656967 @default.
- W2113761719 creator A5086175851 @default.
- W2113761719 date "2004-11-10" @default.
- W2113761719 modified "2023-09-24" @default.
- W2113761719 title "Deficiency of oncoretrovirally transduced hematopoietic stem cells and correction through<i>ex vivo</i>expansion" @default.
- W2113761719 cites W132845342 @default.
- W2113761719 cites W134011100 @default.
- W2113761719 cites W134922517 @default.
- W2113761719 cites W1480965863 @default.
- W2113761719 cites W1517940049 @default.
- W2113761719 cites W1587502583 @default.
- W2113761719 cites W173907651 @default.
- W2113761719 cites W1760571723 @default.
- W2113761719 cites W1863063793 @default.
- W2113761719 cites W1970182792 @default.
- W2113761719 cites W1972124518 @default.
- W2113761719 cites W1973035870 @default.
- W2113761719 cites W1976246831 @default.
- W2113761719 cites W1977340788 @default.
- W2113761719 cites W1981441452 @default.
- W2113761719 cites W1985192507 @default.
- W2113761719 cites W1997554349 @default.
- W2113761719 cites W2000925826 @default.
- W2113761719 cites W2003761566 @default.
- W2113761719 cites W2011917769 @default.
- W2113761719 cites W2013908437 @default.
- W2113761719 cites W2021550815 @default.
- W2113761719 cites W2021923391 @default.
- W2113761719 cites W2080400486 @default.
- W2113761719 cites W2083459784 @default.
- W2113761719 cites W2083632015 @default.
- W2113761719 cites W2088255007 @default.
- W2113761719 cites W2089894429 @default.
- W2113761719 cites W2102085111 @default.
- W2113761719 cites W2105007068 @default.
- W2113761719 cites W2105900939 @default.
- W2113761719 cites W2111759041 @default.
- W2113761719 cites W2137440275 @default.
- W2113761719 cites W2143729842 @default.
- W2113761719 cites W2155509066 @default.
- W2113761719 cites W2173032183 @default.
- W2113761719 cites W2260864551 @default.
- W2113761719 cites W2290402170 @default.
- W2113761719 cites W2292370633 @default.
- W2113761719 cites W2413131581 @default.
- W2113761719 cites W349963634 @default.
- W2113761719 cites W41807445 @default.
- W2113761719 cites W4230721068 @default.
- W2113761719 cites W4249404526 @default.
- W2113761719 cites W80918744 @default.
- W2113761719 doi "https://doi.org/10.1002/jgm.658" @default.
- W2113761719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15538726" @default.
- W2113761719 hasPublicationYear "2004" @default.
- W2113761719 type Work @default.
- W2113761719 sameAs 2113761719 @default.
- W2113761719 citedByCount "4" @default.
- W2113761719 countsByYear W21137617192013 @default.
- W2113761719 countsByYear W21137617192018 @default.
- W2113761719 countsByYear W21137617192022 @default.
- W2113761719 crossrefType "journal-article" @default.
- W2113761719 hasAuthorship W2113761719A5003918131 @default.
- W2113761719 hasAuthorship W2113761719A5041041189 @default.
- W2113761719 hasAuthorship W2113761719A5046656967 @default.
- W2113761719 hasAuthorship W2113761719A5086175851 @default.
- W2113761719 hasConcept C104317684 @default.
- W2113761719 hasConcept C109159458 @default.
- W2113761719 hasConcept C111599444 @default.
- W2113761719 hasConcept C15152581 @default.
- W2113761719 hasConcept C203014093 @default.
- W2113761719 hasConcept C207001950 @default.
- W2113761719 hasConcept C26291073 @default.
- W2113761719 hasConcept C2778086970 @default.
- W2113761719 hasConcept C28328180 @default.
- W2113761719 hasConcept C502942594 @default.
- W2113761719 hasConcept C54355233 @default.
- W2113761719 hasConcept C55493867 @default.
- W2113761719 hasConcept C86803240 @default.
- W2113761719 hasConcept C95444343 @default.
- W2113761719 hasConceptScore W2113761719C104317684 @default.
- W2113761719 hasConceptScore W2113761719C109159458 @default.
- W2113761719 hasConceptScore W2113761719C111599444 @default.
- W2113761719 hasConceptScore W2113761719C15152581 @default.
- W2113761719 hasConceptScore W2113761719C203014093 @default.
- W2113761719 hasConceptScore W2113761719C207001950 @default.
- W2113761719 hasConceptScore W2113761719C26291073 @default.
- W2113761719 hasConceptScore W2113761719C2778086970 @default.
- W2113761719 hasConceptScore W2113761719C28328180 @default.
- W2113761719 hasConceptScore W2113761719C502942594 @default.
- W2113761719 hasConceptScore W2113761719C54355233 @default.
- W2113761719 hasConceptScore W2113761719C55493867 @default.
- W2113761719 hasConceptScore W2113761719C86803240 @default.
- W2113761719 hasConceptScore W2113761719C95444343 @default.
- W2113761719 hasIssue "2" @default.
- W2113761719 hasLocation W21137617191 @default.