Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113766384> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2113766384 endingPage "101" @default.
- W2113766384 startingPage "87" @default.
- W2113766384 abstract "The glutathione S-transferases (EC 2.5.1.18) have been purified to electrophoretic homogeneity from 105,000g supernatant of sheep liver homogenate by employing a combination of gel filtration on Sephadex G-150 and affinity chromatography on S-hexylglutathione-linked Sepharose-6B columns. Approximately 70% of the original glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene and glutathione peroxidase activity toward cumene hydroperoxide could be recovered by this purification method. Of particular importance in developing this procedure was the fact that the enzyme preparation obtained after affinity column chromatography represented all the isozymes of sheep liver glutathione S-transferases. Further purification by CM-cellulose and DEAE-cellulose column chromatography resolved the glutathione S-transferases into seven distinct cationic isozymes designated C-1, C-2, C-3, C-4, C-5, C-6, and C-7 and five overlapping anionic transferases designated A-1, A-2, A-3, A-4, and A-5, respectively, in the order of their elution from the ion-exchange columns. The sodium dodecyl sulfate SDS-gel electrophoretic data on subunit composition revealed that cationic enzymes are composed of two subunits with an identical Mr of 24,000 whereas a predominant subunit with Mr of 26,000 was observed in all anionic isozyme peaks except A-1. Cationic isozymes accounted for approximately 98% of the total peroxidase activity associated with the glutathione S-transferase whereas only A-1 of the anionic isozymes displayed some peroxidase activity. Isozyme C-4 was found to be the most abundant glutathione S-transferase in the sheep liver. Characterization of the individual transferases by their specificity toward a number of selected substrates, subunit composition, and isoelectric points showed some similarities to those patterns for human liver glutathione S-transferases." @default.
- W2113766384 created "2016-06-24" @default.
- W2113766384 creator A5002026660 @default.
- W2113766384 creator A5002045094 @default.
- W2113766384 creator A5002706685 @default.
- W2113766384 creator A5032647042 @default.
- W2113766384 creator A5091109344 @default.
- W2113766384 date "1983-07-01" @default.
- W2113766384 modified "2023-10-17" @default.
- W2113766384 title "Purification and characterization of the individual glutathione S-transferases from sheep liver" @default.
- W2113766384 cites W1428827448 @default.
- W2113766384 cites W1560354598 @default.
- W2113766384 cites W1581414347 @default.
- W2113766384 cites W1588950136 @default.
- W2113766384 cites W173553954 @default.
- W2113766384 cites W1775749144 @default.
- W2113766384 cites W1967754284 @default.
- W2113766384 cites W1969728423 @default.
- W2113766384 cites W1975980695 @default.
- W2113766384 cites W2001183374 @default.
- W2113766384 cites W2032249253 @default.
- W2113766384 cites W2040930057 @default.
- W2113766384 cites W2048220521 @default.
- W2113766384 cites W2100837269 @default.
- W2113766384 cites W2136839207 @default.
- W2113766384 cites W2152141827 @default.
- W2113766384 cites W2411768669 @default.
- W2113766384 cites W30533299 @default.
- W2113766384 cites W317728824 @default.
- W2113766384 doi "https://doi.org/10.1016/0003-9861(83)90192-3" @default.
- W2113766384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6870266" @default.
- W2113766384 hasPublicationYear "1983" @default.
- W2113766384 type Work @default.
- W2113766384 sameAs 2113766384 @default.
- W2113766384 citedByCount "57" @default.
- W2113766384 countsByYear W21137663842012 @default.
- W2113766384 countsByYear W21137663842013 @default.
- W2113766384 countsByYear W21137663842014 @default.
- W2113766384 countsByYear W21137663842018 @default.
- W2113766384 crossrefType "journal-article" @default.
- W2113766384 hasAuthorship W2113766384A5002026660 @default.
- W2113766384 hasAuthorship W2113766384A5002045094 @default.
- W2113766384 hasAuthorship W2113766384A5002706685 @default.
- W2113766384 hasAuthorship W2113766384A5032647042 @default.
- W2113766384 hasAuthorship W2113766384A5091109344 @default.
- W2113766384 hasConcept C119795356 @default.
- W2113766384 hasConcept C161790260 @default.
- W2113766384 hasConcept C162008176 @default.
- W2113766384 hasConcept C163994747 @default.
- W2113766384 hasConcept C179247698 @default.
- W2113766384 hasConcept C181199279 @default.
- W2113766384 hasConcept C185592680 @default.
- W2113766384 hasConcept C193363816 @default.
- W2113766384 hasConcept C2776907368 @default.
- W2113766384 hasConcept C2778113725 @default.
- W2113766384 hasConcept C2779881004 @default.
- W2113766384 hasConcept C43617362 @default.
- W2113766384 hasConcept C538909803 @default.
- W2113766384 hasConcept C55493867 @default.
- W2113766384 hasConceptScore W2113766384C119795356 @default.
- W2113766384 hasConceptScore W2113766384C161790260 @default.
- W2113766384 hasConceptScore W2113766384C162008176 @default.
- W2113766384 hasConceptScore W2113766384C163994747 @default.
- W2113766384 hasConceptScore W2113766384C179247698 @default.
- W2113766384 hasConceptScore W2113766384C181199279 @default.
- W2113766384 hasConceptScore W2113766384C185592680 @default.
- W2113766384 hasConceptScore W2113766384C193363816 @default.
- W2113766384 hasConceptScore W2113766384C2776907368 @default.
- W2113766384 hasConceptScore W2113766384C2778113725 @default.
- W2113766384 hasConceptScore W2113766384C2779881004 @default.
- W2113766384 hasConceptScore W2113766384C43617362 @default.
- W2113766384 hasConceptScore W2113766384C538909803 @default.
- W2113766384 hasConceptScore W2113766384C55493867 @default.
- W2113766384 hasIssue "1" @default.
- W2113766384 hasLocation W21137663841 @default.
- W2113766384 hasLocation W21137663842 @default.
- W2113766384 hasOpenAccess W2113766384 @default.
- W2113766384 hasPrimaryLocation W21137663841 @default.
- W2113766384 hasRelatedWork W1972107437 @default.
- W2113766384 hasRelatedWork W2006492938 @default.
- W2113766384 hasRelatedWork W2007117500 @default.
- W2113766384 hasRelatedWork W2020695834 @default.
- W2113766384 hasRelatedWork W2041812814 @default.
- W2113766384 hasRelatedWork W2048507159 @default.
- W2113766384 hasRelatedWork W2050442603 @default.
- W2113766384 hasRelatedWork W2053458793 @default.
- W2113766384 hasRelatedWork W2089044941 @default.
- W2113766384 hasRelatedWork W2313445687 @default.
- W2113766384 hasVolume "224" @default.
- W2113766384 isParatext "false" @default.
- W2113766384 isRetracted "false" @default.
- W2113766384 magId "2113766384" @default.
- W2113766384 workType "article" @default.