FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2113808564> ?p ?o ?g. }

• W2113808564 endingPage "2772" @default.
• W2113808564 startingPage "2762" @default.
• W2113808564 abstract "ABSTRACT Uropathogenic Escherichia coli (UPEC) causes most community-acquired and nosocomial urinary tract infections (UTI). In a mouse model of UTI, UPEC invades superficial bladder cells and proliferates rapidly, forming biofilm-like structures called intracellular bacterial communities (IBCs). Using a gentamicin protection assay and fluorescence microscopy, we developed an in vitro model for studying UPEC proliferation within immortalized human urothelial cells. By pharmacologic manipulation of urothelial cells with the cholesterol-sequestering drug filipin, numbers of intracellular UPEC CFU increased 8 h and 24 h postinfection relative to untreated cultures. Enhanced UPEC intracellular proliferation required that the urothelial cells, but not the bacteria, be filipin treated prior to infection. However, neither UPEC frequency of invasion nor early intracellular trafficking events to a Lamp1-positive compartment were modulated by filipin. Upon inspection by fluorescence microscopy, cultures with enhanced UPEC intracellular proliferation exhibited large, dense bacterial aggregates within cells that resembled IBCs but were contained with Lamp1-positive vacuoles. While an isogenic fimH mutant was capable of forming these IBC-like structures, the mutant formed significantly fewer than wild-type UPEC. Similar to IBCs, expression of E. coli iron acquisition systems was upregulated by intracellular UPEC. Expression of other putative virulence factors, including hlyA , cnf1 , fliC , kpsD , and the biofilm adhesin yfaL also increased, while expression of fimA decreased and that of flu did not change. These results indicate that UPEC differentially regulates virulence factors in the intracellular environment. Thus, immortalized urothelial cultures that recapitulate IBC formation in vitro represent a novel system for the molecular and biochemical characterization of the UPEC intracellular life cycle." @default.
• W2113808564 created "2016-06-24" @default.
• W2113808564 creator A5018677447 @default.
• W2113808564 creator A5057958322 @default.
• W2113808564 creator A5083040184 @default.
• W2113808564 date "2009-07-01" @default.
• W2113808564 modified "2023-10-05" @default.
• W2113808564 title "Urothelial Cultures Support Intracellular Bacterial Community Formation by Uropathogenic <i>Escherichia coli</i>" @default.
• W2113808564 cites W1480735239 @default.
• W2113808564 cites W1495826882 @default.
• W2113808564 cites W1515778218 @default.
• W2113808564 cites W1647404354 @default.
• W2113808564 cites W1819875891 @default.
• W2113808564 cites W1958495494 @default.
• W2113808564 cites W1968464040 @default.
• W2113808564 cites W1971696954 @default.
• W2113808564 cites W1973041279 @default.
• W2113808564 cites W1975260420 @default.
• W2113808564 cites W1980734735 @default.
• W2113808564 cites W1980904413 @default.
• W2113808564 cites W1985712951 @default.
• W2113808564 cites W1992057102 @default.
• W2113808564 cites W2001297021 @default.
• W2113808564 cites W2001346590 @default.
• W2113808564 cites W2002232188 @default.
• W2113808564 cites W2017296879 @default.
• W2113808564 cites W2019875425 @default.
• W2113808564 cites W2024386331 @default.
• W2113808564 cites W2031458021 @default.
• W2113808564 cites W2036083275 @default.
• W2113808564 cites W2037656756 @default.
• W2113808564 cites W2037753522 @default.
• W2113808564 cites W2038667562 @default.
• W2113808564 cites W2044357040 @default.
• W2113808564 cites W2046721427 @default.
• W2113808564 cites W2047174321 @default.
• W2113808564 cites W2051467330 @default.
• W2113808564 cites W2054020689 @default.
• W2113808564 cites W2057607176 @default.
• W2113808564 cites W2062694727 @default.
• W2113808564 cites W2068711603 @default.
• W2113808564 cites W2068760392 @default.
• W2113808564 cites W2074057696 @default.
• W2113808564 cites W2084511539 @default.
• W2113808564 cites W2086808983 @default.
• W2113808564 cites W2088566842 @default.
• W2113808564 cites W2096770031 @default.
• W2113808564 cites W2101333433 @default.
• W2113808564 cites W2103358704 @default.
• W2113808564 cites W2108619515 @default.
• W2113808564 cites W2110471851 @default.
• W2113808564 cites W2118051267 @default.
• W2113808564 cites W2119836979 @default.
• W2113808564 cites W2123565122 @default.
• W2113808564 cites W2124519865 @default.
• W2113808564 cites W2124772160 @default.
• W2113808564 cites W2127789620 @default.
• W2113808564 cites W2128639098 @default.
• W2113808564 cites W2131227201 @default.
• W2113808564 cites W2136296923 @default.
• W2113808564 cites W2139096665 @default.
• W2113808564 cites W2144098483 @default.
• W2113808564 cites W2144850233 @default.
• W2113808564 cites W2145939196 @default.
• W2113808564 cites W2149611157 @default.
• W2113808564 cites W2151603653 @default.
• W2113808564 cites W2151645494 @default.
• W2113808564 cites W2154075906 @default.
• W2113808564 cites W2162749433 @default.
• W2113808564 cites W2166419456 @default.
• W2113808564 cites W2167675343 @default.
• W2113808564 cites W2168398710 @default.
• W2113808564 cites W2169356779 @default.
• W2113808564 cites W2170356321 @default.
• W2113808564 cites W2171200456 @default.
• W2113808564 cites W2186659781 @default.
• W2113808564 cites W2332961262 @default.
• W2113808564 doi "https://doi.org/10.1128/iai.00323-09" @default.
• W2113808564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2708588" @default.
• W2113808564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19451249" @default.
• W2113808564 hasPublicationYear "2009" @default.
• W2113808564 type Work @default.
• W2113808564 sameAs 2113808564 @default.
• W2113808564 citedByCount "109" @default.
• W2113808564 countsByYear W21138085642012 @default.
• W2113808564 countsByYear W21138085642013 @default.
• W2113808564 countsByYear W21138085642014 @default.
• W2113808564 countsByYear W21138085642015 @default.
• W2113808564 countsByYear W21138085642016 @default.
• W2113808564 countsByYear W21138085642017 @default.
• W2113808564 countsByYear W21138085642018 @default.
• W2113808564 countsByYear W21138085642019 @default.
• W2113808564 countsByYear W21138085642020 @default.
• W2113808564 countsByYear W21138085642021 @default.
• W2113808564 countsByYear W21138085642022 @default.
• W2113808564 countsByYear W21138085642023 @default.
• W2113808564 crossrefType "journal-article" @default.
• W2113808564 hasAuthorship W2113808564A5018677447 @default.

• next