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- W2113860870 abstract "β-Glucuronidase and glucuronyl transferase activities were determined in various stages of third instar larvae and pupae of M. domestica. Most of the β-glucuronidase activity was found in the particulate fractions: lysosomes and microsomes. No enzyme activity could be detected in the early third instar larvae. It increased by 10-fold in the pupal stage and has a pH optimum of 4.0. The enzyme activity in the soluble fraction increased from 30% in the late larval stage to 45% in the late pupal stage. About 56% of lysosomal β-glucuronidase activity exhibited structure-linked latency. The free activity of the lysosomal fractions increased from 44% in the late larval stage to over 64%, in the late pupal stage. Glucuronyl transferase activity was low at all times during metamorphosis. The rapid rate of β-glucuronidase synthesis during pupal and late pupal stages and the increase in the lysosomal free activity has been discussed in relation to the cellular dissolution and tissue reconstitution during metamorphosis." @default.
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- W2113860870 date "1969-01-01" @default.
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- W2113860870 title "Intracellular distribution of β-glucuronidase activity during metamorphosis of the housefly, <i>Musca domestica</i> L." @default.
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- W2113860870 doi "https://doi.org/10.1139/z69-010" @default.
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