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- W2113996040 abstract "Abstract A system consisting of donor (1), template (2) and immobilized acceptor molecules (58,59,64-67) which may be used for an enzymatic extension (T4 DNA ligase) of immobilized DNA fragments is described. The synthesis of the donor (1), template (2) and the functionalized acceptor molecules (3-8) was performed via phosphotriester intermediates. The functionalized acceptor molecules used in this study contained: base labile bonds (i.e. compounds 3-6), an acid labile bond (i.e. compound 7) or a RNase labile bond (i.e. compound 8). The functionalized acceptor molecules (3,4) could be immobilized to cellulose activated with 2,4,6-trichloro-s-triazine and the other molecules (5-8) to 2-amino-4, 6-dichloro-s-triazine to give the immobilized DNA complexes 58, 59 and 64-67, respectively, in high yield. The immobilized DNA fragments could be released quantitatively from the solid support by: base treatment (i.e. 58, 59, 64 and 65 gave DNA-fragments 60, 61, 68 and 69 respectively), acid treatment (i.e. 66 gave DNA-fragment 70) or RNase digestion (i.e. 67 gave deoxythymidine)." @default.
- W2113996040 created "2016-06-24" @default.
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- W2113996040 date "1979-01-01" @default.
- W2113996040 modified "2023-10-18" @default.
- W2113996040 title "Synthesis of functionalized dna fragments suitable for reversible attachment to activated cellulose" @default.
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- W2113996040 doi "https://doi.org/10.1016/s0040-4020(01)99509-2" @default.
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