FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114028473> ?p ?o ?g. }

• W2114028473 endingPage "2497" @default.
• W2114028473 startingPage "2485" @default.
• W2114028473 abstract "The conformational change observed upon ligand binding and phosphorylation for the cAMP-dependent protein kinase (protein kinase A-PKA) is of high importance for the regulation of its activity. We calculate pKa values and net charges for 18 3D structures of PKA in various conformations and liganded states to examine the role of electrostatics in ligand binding and activation. We find that the conformational change of PKA takes place without any significant net proton uptake/release at all pH values, thus indicating that PKA has evolved to reduce any pH-dependent barriers to the conformational motion. We furthermore find that the binding of ligands induces large changes in the net charge of PKA at most pH values, but significantly, we find that the net charge difference at physiological pH is close to zero, thus indicating that the active-site pKa values have been preorganized for substrate binding. We are unable to unequivocally resolve the identity of the groups responsible for determining the pH-activity profile of PKA but speculate that the titration of Lys 168 or the titration of ATP itself could be responsible for the loss of activity at high pH values. Finally, we examine the effect of point mutations on the pKa values of the PKA catalytic residues and find these to be relatively insensitive to both noncharge-altering and charge-altering mutations." @default.
• W2114028473 created "2016-06-24" @default.
• W2114028473 creator A5051774578 @default.
• W2114028473 creator A5082362785 @default.
• W2114028473 date "2010-11-18" @default.
• W2114028473 modified "2023-10-10" @default.
• W2114028473 title "Calculating pKa values in the cAMP-dependent protein kinase: The effect of conformational change and ligand binding" @default.
• W2114028473 cites W1793492141 @default.
• W2114028473 cites W1942192923 @default.
• W2114028473 cites W1969015820 @default.
• W2114028473 cites W1993141548 @default.
• W2114028473 cites W1994116487 @default.
• W2114028473 cites W2015074746 @default.
• W2114028473 cites W2023245036 @default.
• W2114028473 cites W2029582401 @default.
• W2114028473 cites W2030205108 @default.
• W2114028473 cites W2032590393 @default.
• W2114028473 cites W2033630329 @default.
• W2114028473 cites W2039665613 @default.
• W2114028473 cites W2041399083 @default.
• W2114028473 cites W2058943073 @default.
• W2114028473 cites W2060059149 @default.
• W2114028473 cites W2062630094 @default.
• W2114028473 cites W2065939630 @default.
• W2114028473 cites W2067405360 @default.
• W2114028473 cites W2068153876 @default.
• W2114028473 cites W2074273453 @default.
• W2114028473 cites W2076790509 @default.
• W2114028473 cites W2077221346 @default.
• W2114028473 cites W2078505391 @default.
• W2114028473 cites W2087593382 @default.
• W2114028473 cites W2093084945 @default.
• W2114028473 cites W2098411183 @default.
• W2114028473 cites W2099099776 @default.
• W2114028473 cites W2114367386 @default.
• W2114028473 cites W2114764346 @default.
• W2114028473 cites W2114788392 @default.
• W2114028473 cites W2125531202 @default.
• W2114028473 cites W2127116852 @default.
• W2114028473 cites W2130479394 @default.
• W2114028473 cites W2132717858 @default.
• W2114028473 cites W2133558724 @default.
• W2114028473 cites W2138778824 @default.
• W2114028473 cites W2146593541 @default.
• W2114028473 cites W2151946950 @default.
• W2114028473 cites W2152054228 @default.
• W2114028473 cites W2152997175 @default.
• W2114028473 cites W2155163585 @default.
• W2114028473 cites W2157538153 @default.
• W2114028473 cites W2166478478 @default.
• W2114028473 cites W2953375613 @default.
• W2114028473 doi "https://doi.org/10.1002/pro.530" @default.
• W2114028473 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3009415" @default.
• W2114028473 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20954248" @default.
• W2114028473 hasPublicationYear "2010" @default.
• W2114028473 type Work @default.
• W2114028473 sameAs 2114028473 @default.
• W2114028473 citedByCount "4" @default.
• W2114028473 countsByYear W21140284732012 @default.
• W2114028473 countsByYear W21140284732013 @default.
• W2114028473 crossrefType "journal-article" @default.
• W2114028473 hasAuthorship W2114028473A5051774578 @default.
• W2114028473 hasAuthorship W2114028473A5082362785 @default.
• W2114028473 hasBestOaLocation W21140284732 @default.
• W2114028473 hasConcept C116569031 @default.
• W2114028473 hasConcept C117626034 @default.
• W2114028473 hasConcept C119599485 @default.
• W2114028473 hasConcept C11960822 @default.
• W2114028473 hasConcept C12554922 @default.
• W2114028473 hasConcept C127413603 @default.
• W2114028473 hasConcept C147789679 @default.
• W2114028473 hasConcept C170493617 @default.
• W2114028473 hasConcept C179104552 @default.
• W2114028473 hasConcept C184866935 @default.
• W2114028473 hasConcept C185592680 @default.
• W2114028473 hasConcept C18903297 @default.
• W2114028473 hasConcept C2777289219 @default.
• W2114028473 hasConcept C2777339483 @default.
• W2114028473 hasConcept C55493867 @default.
• W2114028473 hasConcept C59244509 @default.
• W2114028473 hasConcept C71240020 @default.
• W2114028473 hasConcept C86803240 @default.
• W2114028473 hasConcept C87716614 @default.
• W2114028473 hasConcept C97029542 @default.
• W2114028473 hasConceptScore W2114028473C116569031 @default.
• W2114028473 hasConceptScore W2114028473C117626034 @default.
• W2114028473 hasConceptScore W2114028473C119599485 @default.
• W2114028473 hasConceptScore W2114028473C11960822 @default.
• W2114028473 hasConceptScore W2114028473C12554922 @default.
• W2114028473 hasConceptScore W2114028473C127413603 @default.
• W2114028473 hasConceptScore W2114028473C147789679 @default.
• W2114028473 hasConceptScore W2114028473C170493617 @default.
• W2114028473 hasConceptScore W2114028473C179104552 @default.
• W2114028473 hasConceptScore W2114028473C184866935 @default.
• W2114028473 hasConceptScore W2114028473C185592680 @default.
• W2114028473 hasConceptScore W2114028473C18903297 @default.
• W2114028473 hasConceptScore W2114028473C2777289219 @default.
• W2114028473 hasConceptScore W2114028473C2777339483 @default.

• next