FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114035610> ?p ?o ?g. }

• W2114035610 endingPage "3814" @default.
• W2114035610 startingPage "3814" @default.
• W2114035610 abstract "Peptide arrays have emerged as a key technology for drug discovery, diagnosis, and cell biology. Despite the promise of these arrays, applications of peptide arrays to quantitative analysis of enzyme kinetics have been limited due to the difficulty in obtaining quantitative information of enzymatic reaction products. In this study, we developed a new approach for the quantitative kinetics analysis of proteases using fluorescence-conjugated peptide arrays, a surface concentration-based assay with solid-phase peptide standards using dry-off measurements, and compared it with an applied concentration-based assay. For fabrication of the peptide arrays, substrate peptides of cMMP-3, caspase-3, caspase-9, and calpain-1 were functionalized with TAMRA and cysteine, and were immobilized onto amine-functionalized arrays using a heterobifunctional linker, N-[γ-maleimidobutyloxy]succinimide ester. The proteolytic activities of the four enzymes were quantitatively analyzed by calculating changes induced by enzymatic reactions in the concentrations of peptides bound to array surfaces. In addition, this assay was successfully applied for calculating the Michaelis constant (Km,surf) for the four enzymes. Thus, this new assay has a strong potential for use in the quantitative evaluation of proteases, and for drug discovery through kinetics studies including the determination of Km and Vmax." @default.
• W2114035610 created "2016-06-24" @default.
• W2114035610 creator A5005622095 @default.
• W2114035610 creator A5013179307 @default.
• W2114035610 creator A5053273074 @default.
• W2114035610 creator A5054964892 @default.
• W2114035610 creator A5058101079 @default.
• W2114035610 date "2012-01-01" @default.
• W2114035610 modified "2023-10-14" @default.
• W2114035610 title "Quantitative kinetics of proteolytic enzymes determined by a surface concentration-based assay using peptide arrays" @default.
• W2114035610 cites W1963702379 @default.
• W2114035610 cites W1978059786 @default.
• W2114035610 cites W1982013048 @default.
• W2114035610 cites W1983044337 @default.
• W2114035610 cites W1983476428 @default.
• W2114035610 cites W1989680993 @default.
• W2114035610 cites W1991582404 @default.
• W2114035610 cites W1998169020 @default.
• W2114035610 cites W2002455537 @default.
• W2114035610 cites W2003375281 @default.
• W2114035610 cites W2009682872 @default.
• W2114035610 cites W2010912389 @default.
• W2114035610 cites W2030457901 @default.
• W2114035610 cites W2035605707 @default.
• W2114035610 cites W2049202753 @default.
• W2114035610 cites W2051155311 @default.
• W2114035610 cites W2064059972 @default.
• W2114035610 cites W2069508712 @default.
• W2114035610 cites W2071359561 @default.
• W2114035610 cites W2074901788 @default.
• W2114035610 cites W2077337492 @default.
• W2114035610 cites W2077550665 @default.
• W2114035610 cites W2083015173 @default.
• W2114035610 cites W2086218705 @default.
• W2114035610 cites W2092602198 @default.
• W2114035610 cites W2093662743 @default.
• W2114035610 cites W2156721247 @default.
• W2114035610 cites W2159825665 @default.
• W2114035610 cites W2327509943 @default.
• W2114035610 cites W4211014836 @default.
• W2114035610 doi "https://doi.org/10.1039/c2an35080g" @default.
• W2114035610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22768390" @default.
• W2114035610 hasPublicationYear "2012" @default.
• W2114035610 type Work @default.
• W2114035610 sameAs 2114035610 @default.
• W2114035610 citedByCount "16" @default.
• W2114035610 countsByYear W21140356102012 @default.
• W2114035610 countsByYear W21140356102013 @default.
• W2114035610 countsByYear W21140356102014 @default.
• W2114035610 countsByYear W21140356102015 @default.
• W2114035610 countsByYear W21140356102016 @default.
• W2114035610 countsByYear W21140356102018 @default.
• W2114035610 countsByYear W21140356102021 @default.
• W2114035610 countsByYear W21140356102023 @default.
• W2114035610 crossrefType "journal-article" @default.
• W2114035610 hasAuthorship W2114035610A5005622095 @default.
• W2114035610 hasAuthorship W2114035610A5013179307 @default.
• W2114035610 hasAuthorship W2114035610A5053273074 @default.
• W2114035610 hasAuthorship W2114035610A5054964892 @default.
• W2114035610 hasAuthorship W2114035610A5058101079 @default.
• W2114035610 hasConcept C111919701 @default.
• W2114035610 hasConcept C121332964 @default.
• W2114035610 hasConcept C131779359 @default.
• W2114035610 hasConcept C148898269 @default.
• W2114035610 hasConcept C178790620 @default.
• W2114035610 hasConcept C181199279 @default.
• W2114035610 hasConcept C182220744 @default.
• W2114035610 hasConcept C185592680 @default.
• W2114035610 hasConcept C18903297 @default.
• W2114035610 hasConcept C21951064 @default.
• W2114035610 hasConcept C2776830054 @default.
• W2114035610 hasConcept C2777289219 @default.
• W2114035610 hasConcept C2779201268 @default.
• W2114035610 hasConcept C2779281246 @default.
• W2114035610 hasConcept C2780557392 @default.
• W2114035610 hasConcept C3019223276 @default.
• W2114035610 hasConcept C41008148 @default.
• W2114035610 hasConcept C41183919 @default.
• W2114035610 hasConcept C43617362 @default.
• W2114035610 hasConcept C55493867 @default.
• W2114035610 hasConcept C56856141 @default.
• W2114035610 hasConcept C62520636 @default.
• W2114035610 hasConcept C86803240 @default.
• W2114035610 hasConcept C95986675 @default.
• W2114035610 hasConceptScore W2114035610C111919701 @default.
• W2114035610 hasConceptScore W2114035610C121332964 @default.
• W2114035610 hasConceptScore W2114035610C131779359 @default.
• W2114035610 hasConceptScore W2114035610C148898269 @default.
• W2114035610 hasConceptScore W2114035610C178790620 @default.
• W2114035610 hasConceptScore W2114035610C181199279 @default.
• W2114035610 hasConceptScore W2114035610C182220744 @default.
• W2114035610 hasConceptScore W2114035610C185592680 @default.
• W2114035610 hasConceptScore W2114035610C18903297 @default.
• W2114035610 hasConceptScore W2114035610C21951064 @default.
• W2114035610 hasConceptScore W2114035610C2776830054 @default.
• W2114035610 hasConceptScore W2114035610C2777289219 @default.
• W2114035610 hasConceptScore W2114035610C2779201268 @default.
• W2114035610 hasConceptScore W2114035610C2779281246 @default.

• next